Remote sensing and climate data as a key for understanding fasciolosis transmission in the Andes: review and update of an ongoing interdisciplinary project

Fasciolosis caused by Fasciola hepatica in various South American countries located on the slopes of the Andes has been recognized as an important public health problem. However, the importance of this zoonotic hepatic parasite was neglected until the last decade. Countries such as Peru and Bolivia are considered to be hyperendemic areas for human and animal fasciolosis, and other countries such as Chile, Ecuador, Colombia and Venezuela are also affected. At the beginning of the 1990s a multidisciplinary project was launched with the aim to shed light on the problems related to this parasitic disease in the Northern Bolivian Altiplano. A few years later, a geographic information system (GIS) was incorporated into this multidisciplinary project analysing the epidemiology of human and animal fasciolosis in this South American Andean region. Various GIS projects were developed in some Andean regions using climatic data, climatic forecast indices and remote sensing data. Step by step, all these GIS projects concerning the forecast of the fasciolosis transmission risk in the Andean mountain range were revised and in some cases updated taking into account new data. The first of these projects was developed on a regional scale for the central Chilean regions and the proposed model was validated on a local scale in the Northern Bolivian Altiplano. This validated mixed model, based on both fasciolosis climatic forecast indices and normalized difference vegetation index values from Advanced Very High Resolution Radiometer satellite sensor, was extrapolated to other human and/or animal endemic areas of Peru and Ecuador. The resulting fasciolosis risk maps make it possible to show the known human endemic areas of, mainly, the Peruvian Altiplano, Cajamarca and Mantaro Peruvian valleys, and some valleys of the Ecuadorian Cotopaxi province. Nevertheless, more climate and remote sensing data, as well as more accurate epidemiological reports, have to be incorporated into these GIS projects, which should be considered the key in understanding fasciolosis transmission in the Andes

Nematode Parasites of the European Pilchard, Sardina pilchardus (Walbaum, 1792): A Genuine Human Hazard?

The European pilchard is one of the most frequently consumed fish species in Mediterranean countries, especially in Italy and Spain, and has been reported as the cause of at least eight human anisakidosis cases in Spain since 1991, the parasitic disease caused by the ingestion of fish or cephalopods infested by the larval stage of anisakid nematodes. With the aim to shed light on the potential human parasitosis risk posed by these nematode larvae, we helminthologically analyzed a total of 350 sardines (European pilchard) captured in the Atlantic Ocean (175 sardines) and the Mediterranean Sea (175 specimens), acquired in various Spanish nationwide supermarket chains. The statistical analysis of some helminth parameters revealed a higher presence of nematodes belonging to the genus Hysterothylacium (frequency of parasitation of 24.29%; total mean parasite burden of 2.36), usually considered non-parasitic for humans (only three cases reported worldwide), when compared to nematodes of the genus Anisakis (5.71%; 0.16). The human anisakidosis risk after the consumption of raw or undercooked sardines and the role of Hysterothylacium, the most frequent nematode, is discussed, providing information to consumers. To avoid human infection by anisakid larval nematodes, the established preventive measures are confirmed and new ones are proposed

Molecular detection of Leishmania infantum in rats and sand flies in the urban sewers of Barcelona, Spain

Background: Classically, dogs have been considered to be the only reservoir of leishmaniasis in urban areas. However, in a previous study, we found a 33.3% prevalence of Leishmania infantum in the spleens of Norway rats (Rattus norvegicus) sampled in the underground sewer system of the city of Barcelona (Spain). The aim of the present study was to verify, using molecular methods, the potential reservoir role of these rats in the same sewer system. Methods: A sensitive real-time PCR (qPCR) assay, DNA sequencing and phylogenetic analysis were carried out to identify and quantify the presence of L. infantum DNA in sand fly individuals captured in the same underground sewer system of Barcelona as in our previous study and in the spleens and ears of rats captured in the same sewer system. Results: Leishmania infantum DNA was found in 14 of the 27 (51.9%) sand flies identified as Phlebotomus perniciosus, and 10 of the 24 (41.7%) rats studied were infected. Leishmania infantum was found in the spleens (70%) and in the ears (40%) of the infected rats. Quantitative results revealed the presence of high loads of L. infantum in the rats studied (> 3 × 10 parasites/g ear tissue) and among the sand flies (> 34 × 10 parasites in 1 individual). Conclusions: The molecular methods used in this study demonstrated a high prevalence of L. infantum in the underground sewer populations of both R. norvegicus and P. perniciosus. These results suggest that sewer rats, in addition to dogs, are likely to act as reservoirs of leishmaniasis in cities, where sewer systems seem to offer the ideal scenario for the transmission of leishmaniasis. Therefore, to achieve the WHO 2030 target on the elimination of leishmaniasis as a public health problem successfully, an efficient control strategy against leishmaniasis in rats and sand flies should be implemented, particularly in the sewer systems of urban areas of endemic countries. Graphical Abstract: [Figure not available: see fulltext.

Remote sensing and climate data as a key for understanding fasciolosis transmission in the Andes: review and update of an ongoing interdisciplinary project

Fasciolosis caused by Fasciola hepatica in various South American countries located on the slopes of the Andes has been recognized as an important public health problem. However, the importance of this zoonotic hepatic parasite was neglected until the last decade. Countries such as Peru and Bolivia are considered to be hyperendemic areas for human and animal fasciolosis, and other countries such as Chile, Ecuador, Colombia and Venezuela are also affected. At the beginning of the 1990s a multidisciplinary project was launched with the aim to shed light on the problems related to this parasitic disease in the Northern Bolivian Altiplano. A few years later, a geographic information system (GIS) was incorporated into this multidisciplinary project analysing the epidemiology of human and animal fasciolosis in this South American Andean region. Various GIS projects were developed in some Andean regions using climatic data, climatic forecast indices and remote sensing data. Step by step, all these GIS projects concerning the forecast of the fasciolosis transmission risk in the Andean mountain range were revised and in some cases updated taking into account new data. The first of these projects was developed on a regional scale for the central Chilean regions and the proposed model was validated on a local scale in the Northern Bolivian Altiplano. This validated mixed model, based on both fasciolosis climatic forecast indices and normalized difference vegetation index values from Advanced Very High Resolution Radiometer satellite sensor, was extrapolated to other human and/or animal endemic areas of Peru and Ecuador. The resulting fasciolosis risk maps make it possible to show the known human endemic areas of, mainly, the Peruvian Altiplano, Cajamarca and Mantaro Peruvian valleys, and some valleys of the Ecuadorian Cotopaxi province. Nevertheless, more climate and remote sensing data, as well as more accurate epidemiological reports, have to be incorporated into these GIS projects, which should be considered the key in understanding fasciolosis transmission in the Andes

Socio-environmental variables and transmission risk of lymphatic filariasis in central and northern Mozambique

Lymphatic filariasis (LF) is endemic in Mozambique, where it is caused by Wuchereria bancrofti with Culex quinquefasciatus as the main vector. It affects approximately 10% of the population (2 million) with about 16 million at risk. Prevalence rates in 40 out of 65 districts that together comprise the four endemic provinces Niassa, Cabo Delgado, Nampula and Zambezia were analysed with the aim of elucidating the socio-environmental variables influencing the transmission. The levels of prevalence were divided into six ranks and certain climatic, environmental and social factors were considered independent variables. A climadiagram was created and the LF risk and the water budget-based index were calculated for each district. Factors influencing the risk of the overall transmission and that of the provincial levels were established by discriminant analysis. The results show that LF transmission increased with mean maximum temperature and decreased with altitude. The almost constant annual temperature (especially in the tropical area), altitude, general economic conditions and predominant crop production (rice) were found to be responsible for the abundance and presence of the vector. However, despite the presence of the vector in the hinterland, presence and survival of the parasite were not found to be favoured there. The transmission risk was found to be highest in Zambezia, and consequently also the prevalence, while the situation in Niassa was the opposite. The conclusion is that temperature, altitude and the development/poverty index (particularly in the urban areas) have to be considered as transmission risk factors for LF in Mozambique. The extent of rice culturing probably also plays a role with respect to this infectio

Low-altitude outbreaks of human fascioliasis related with summer rainfall in Gilan province, Iran

Abstract. Following human fascioliasis outbreaks in 1988 and 1999 in Gilan province, northern Iran, efforts are now made to shed light on the seasonal pattern of fascioliasis transmission in this endemic area, taking into account snail host populations, climatic conditions and human cases. Populations of the intermediate host snail (Lymnaea spp.) peak in May and November, while there is a fourfold increase in the rate of human fascioliasis in February compared to that of September. Transmission is likely to occur mainly in late autumn and sporadically in late spring. Rainfall, seasonally analysed in periods of 3 years, indicates that accumulated summer rainfall may be related with the 1988 and 1999 human fascioliasis outbreaks. Although a more detailed picture, based on the analysis of further abiotic and biotic factors influencing fascioliasis transmission in this area, is required to substantiate this hypothesis, our results serve as the first step of a geographical information system project concerning the epidemiological study of fascioliasis in Iran. This local-scale study concerning the effects of climate change and natural disasters on the spread of fascioliasis aims to facilitate the understanding of what goes on at the regional scale in this respect. Keywords: fascioliasis, human outbreak, summer rainfall, Iran. Human fascioliasis is geographically widespread and recognised as a serious public health problem in some endemic areas, which includes the Andean countrie

Spatio-temproal prediction of the malaria transmission risk in Minab district (Hormozgan Province, Southern Iran

Introduction: Malaria is the most important parasitic disease in tropical and subtropical regions, with more than 240 million cases reported annually. In Iran, indigenous cases occur in its south-eastern region. The aim of this study is to assess the environmental risk of malaria transmission in an endemic area of southern Iran. Methods: The study was carried out in Minab district (Hormozgan province, southern Iran), with the aim to assess the environmental risk of malaria, based on a spatio-temporal study, using Growing Degree Days (GDD)-based predictions, larval habitat ecology, MaxEnt spatial predictions and malaria transmission data. Results: The Gradient Model Risk index showed the highest malaria transmission risk period to be during January-April and October-December. The ecological conditions of water bodies of larval habitats of the four vector species (Anopheles culicifacies, A. dthali, A. fluviatilis and A. stephensi) were assessed, with A. stephensi being the most prevalent and the most widely distributed species. Conclusion: These findings, together with the MaxEnt Anopheles predictive distribution models, allowed identifying villages in danger of malaria transmission in Minab district. This spatio-temporal prediction of malaria transmission risk should be incorporated in the design of malaria control initiatives towards a local malaria early warning system. Moreover, the proposed transmission risk model can be extrapolated, at local scale, to other malaria endemic areas of tropical and subtropical regions