Alcohol-induced severe acute pancreatitis followed by hemolytic uremic syndrome managed with continuous renal replacement therapy

BACKGROUND: Acute kidney injury in patients with acute pancreatitis carries a poor prognosis. Hemolytic uremic syndrome (HUS) is characterized by non-immune hemolytic anemia, thrombocytopenia, and renal failure caused by platelet thrombi in the microcirculation of the kidney, and though rare in adults it is associated with high mortality and a high rate of chronic renal failure. CASE PRESENTATION: Herein, we report a case of alcohol-induced acute pancreatitis in a 38-year-old Chinese female complicated by HUS. Her renal function progressively deteriorated in 2 days, and daily continuous renal replacement therapy (CRRT) was thus performed for a total of 13 treatments. She also received intermittent transfusions of fresh frozen plasma. Her renal failure was successfully managed, with subsequent return of normal renal function. She was discharged 1 month after admission and follow-up at 3 months revealed normal urea and creatinine. CONCLUSION: CRRT was shown to be useful for the treatment of HUS following acute pancreatitis. Prior case reports and our case should remind clinicians that HUS is a possible complication of acute pancreatitis. This study highlights the importance of early diagnosis and prompt initiation of CRRT to prevent mortality and improve outcomes

Genetic diversity analysis of cocksfoot (Dactylis glomerata L.) accessions with sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) markers

Sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR), two advanced molecular markers for genetic research in grass and forage, were used to analyze the genetic diversity among 44 accessions of cocksfoot collected from seven countries and regions. 21 SRAP primer combinations generated 476 bands, of which 401 were polymorphic (84.24%). Using 12 ISSR primers, 100 polymorphic bands out of 115 bands in total were generated (86.96%). The coefficient of genetic similarity from SRAP and ISSR data ranged from 0.6838 to 0.9686 and from 0.6935 to 0.9231, respectively. Based on unweighted pair group method with arithmetic mean (UPGMA) cluster and principal component analysis (PCA) on a series of genetic characteristics, all accessions were divided into three clusters and four clusters using two markers, respectively. Those, accessions collected from the identical continent were classified into the same cluster, suggesting the geographical distribution of genetic diversity of cocksfoot. The genetic diversity of Chinese cocksfoot except for three Chinese cultivars was rather rich and greater than that of other regions. We proposed that both SRAP and ISSR markers were considered as useful tools for evaluating the genetic diversity of cocksfoot. Especially, SRAP detected more variance and gave clearer cluster groups.Keywords: Cocksfoot, genetic diversity, sequence-related amplified polymorphism (SRAP), inter-simple sequence repeat (ISSR), germplas

Clearing Persistent Extracellular Antigen of Hepatitis B Virus: An Immunomodulatory Strategy To Reverse Tolerance for an Effective Therapeutic Vaccination

Development of therapeutic vaccines/strategies to control chronic hepatitis B virus (HBV) infection (CHB) has been challenging due to HBV-induced tolerance. In this study, we explored strategies for breaking tolerance and restoring the immune response to the HBV surface antigen in tolerant mice. We demonstrated that immune tolerance status is attributed to the level and duration of circulating HBsAg in HBV carrier models. Removal of circulating HBsAg by a monoclonal anti-HBsAg antibody in tolerant mice could gradually reduce tolerance and reestablish B cell and CD4+ T cell responses to subsequent Engerix-B vaccination, producing protective IgG. Furthermore, HBsAg-specific CD8+ T cells induced by the addition of a TLR agonist, resulted in clearance of HBV in both serum and liver. Thus, generation of protective immunity can be achieved by clearing extracellular viral antigen with neutralizing antibodies followed by vaccination

Effect of polygonimitin C on bone formation and resorption in human osteoblast-like MG63 cells

Purpose: To investigate the effect of polygonimitin C (PC) on bone formation and resorption in human osteoblast-like MG63 cells.Methods: MG63 cells were treated with PC at doses of 0, 20, 40 or 80 μg/mL for 48 h, with an untreated group as control. The effect of PC on alkaline phosphatase (ALP) activity in MG63 cells was investigated by p-nitrophenyl phosphate disodium hexahydrate assay. Western blot assay was used to evaluate the effect of PC on the expressions of osterix (OSX), bone morphogenetic protein-2 (BMP-2), runt-related transcription factor 2 (RUNX-2), osteocalcin (OC), fibronectin (FN), type I collagen (COL I), osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL) proteins in MG63 cells.Results: ALP relative activity in MG63 cells treated with PC at 20, 40 or 80 μg/mL (123.58, 137.74 or 159.62 %, respectively) was significantly (p < 0.05 or 0.01) higher than that in control group (99.37 %). Expressions of OSX, BMP-2, RUNX-2, OC, FN, COL I and OPG proteins in MG63 cells treated with PC at 20, 40 or 80 μg/mL were significantly (p < 0.01) higher than those in control group. However, there were no statistically significant differences in RANKL protein expression between PC-treated MG63 cells and control group.Conclusion: These results show that PC exerts protective effects against osteoporosis by promoting bone formation and inhibiting bone resorption. Thus, PC may be useful in the development of new antiosteoporosis drugs.Keywords: Polygonimitin C, MG63 cells, Bone formation, Bone resorption, Osteoporosi

Intra-Annual Radial Growth of Pinus kesiya var. langbianensis Is Mainly Controlled by Moisture Availability in the Ailao Mountains, Southwestern China

Intra-annual monitoring of tree growth dynamics is increasingly applied to disentangle growth-change relationships with local climate conditions. However, such studies are still very limited in subtropical regions which show a wide variety of climate regimes. We monitored stem radius variations (SRV) of Pinus kesiya var. langbianensis (Szemao pine) over five years (2012–2015 and 2017) in the subtropical monsoon mountain climate of the Ailao Mountains, Yunnan Province, southwest China. On average, the stem radial growth of Szemao pine started in early March and ended in early October, and the highest growth rates occurred during May to June. Stem radius increments were synchronous with precipitation events, while tree water deficit corresponded to the drought periods. Correlation analysis and linear mixed-effects models revealed that precipitation and relative humidity are the most important limiting factors of stem radial increments, whereas air temperature and vapor pressure deficit significantly affected tree water balance and may play an important role in determining the growing season length and seasonality (i.e., duration, start, and cessation). This study reveals that moisture availability plays a major role for tree growth of P. kesiya var langbianensis in the Ailao Mountains, southwest China