Candida albicans repetitive elements display epigenetic diversity and plasticity

Transcriptionally silent heterochromatin is associated with repetitive DNA. It is poorly understood whether and how heterochromatin differs between different organisms and whether its structure can be remodelled in response to environmental signals. Here, we address this question by analysing the chromatin state associated with DNA repeats in the human fungal pathogen Candida albicans. Our analyses indicate that, contrary to model systems, each type of repetitive element is assembled into a distinct chromatin state. Classical Sir2-dependent hypoacetylated and hypomethylated chromatin is associated with the rDNA locus while telomeric regions are assembled into a weak heterochromatin that is only mildly hypoacetylated and hypomethylated. Major Repeat Sequences, a class of tandem repeats, are assembled into an intermediate chromatin state bearing features of both euchromatin and heterochromatin. Marker gene silencing assays and genome-wide RNA sequencing reveals that C. albicans heterochromatin represses expression of repeat-associated coding and non-coding RNAs. We find that telomeric heterochromatin is dynamic and remodelled upon an environmental change. Weak heterochromatin is associated with telomeres at 30?°C, while robust heterochromatin is assembled over these regions at 39?°C, a temperature mimicking moderate fever in the host. Thus in C. albicans, differential chromatin states controls gene expression and epigenetic plasticity is linked to adaptation

Sporormiella as a tool for detecting the presence of large herbivores in the Neotropics

The reliability of using the abundance of Sporormiella spores as a proxy for the presence and abundance of megaherbivores was tested in southern Brazil. Mud-water interface samples from nine lakes, in which cattle-use was categorized as high, medium, or low, were assayed for Sporormiella representation. The sampling design allowed an analysis of both the influence of the number of animals using the shoreline and the distance of the sampling site from the nearest shoreline. Sporormiella was found to be a reliable proxy for the presence of large livestock. The concentration and abundance of spores declined from the edge of the lake toward the center, with the strongest response being in sites with high livestock use. Consistent with prior studies in temperate regions, we find that Sporormiella spores are a useful proxy to study the extinction of Pleistocene megafauna or the arrival of European livestock in Neotropical landscapes

Negative responses of highland pines to anthropogenic activities in inland Spain: a palaeoecological perspective

Palaeoecological evidence indicates that highland pines were dominant in extensive areas of the mountains of Central and Northern Iberia during the first half of the Holocene. However, following several millennia of anthropogenic pressure, their natural ranges are now severely reduced. Although pines have been frequently viewed as first-stage successional species responding positively to human disturbance, some recent palaeobotanical work has proposed fire disturbance and human deforestation as the main drivers of this vegetation turnover. To assess the strength of the evidence for this hypothesis and to identify other possible explanations for this scenario, we review the available information on past vegetation change in the mountains of northern inland Iberia. We have chosen data from several sites that offer good chronological control, including palynological records with microscopic charcoal data and sites with plant macro- and megafossil occurrence. We conclude that although the available long-term data are still fragmentary and that new methods are needed for a better understanding of the ecological history of Iberia, fire events and human activities (probably modulated by climate) have triggered the pine demise at different locations and different temporal scales. In addition, all palaeoxylological, palynological and charcoal results obtained so far are fully compatible with a rapid human-induced ecological change that could have caused a range contraction of highland pines in western Iberia

Emerging issues in biodiversity & conservation management: The need for a palaeoecological perspective

The study of Quaternary environmental change is directly applicable to on-going issues of global conservation. Palaeoecological research techniques provide the tools to address some of the key questions presently being asked by conservation ecologists and land management organizations. But is this type of analysis currently being utilized to its full potential? Are the results of palaeoenvironmental analyses routinely applied to practical issues of natural resource management, and if not what can be done to expand the application of this research within the conservation community? This paper reviews recent developments in the application of the analysis of late Quaternary environmental change to key environmental issues of biodiversity and conservation management and examines areas which could be strengthened in the future including: (i) determination of baselines and natural ecosystem variability; (ii) understanding ecological thresholds and resilience; (iii) climate change conservation strategies; (iv) biological invasions; and (v) conservation and culture. © 2008 Elsevier Ltd. All rights reserved

Lake or bog? Reconstructing baseline ecological conditions for the protected Galápagos <i>Sphagnum</i> peatbogs

This paper documents the first 10,000 year old plant macrofossil record of vegetation changes on the central island of Santa Cruz, providing information on Sphagnum bog vegetation patterns, local extinction of key taxa, and temporal successions in the Galápagos humid highlands. Vegetation change is reconstructed through examination of Holocene sedimentary sequences obtained from three Sphagnum bogs located within volcanic caldera forming the high elevation central ridge system of Santa Cruz Island. Results indicate that these specialized Sphagnum bog ecosystems are dynamic and have undergone considerable changes in vegetation composition, transitioning from diverse hygrophilous herbs and submerged aquatic ecosystems to drier Sphagnum/. Pteridium bog systems, during the last 10,000 cal yr BP. Additionally a new aquatic genus previously undocumented on the islands, Elatine, was discovered at two of the study sites, but it is now extinct on the archipelago. Some of the observed vegetation successions may have been driven by climatic shifts occurring within the eastern equatorial Pacific (EEP). Other drivers including anthropogenic change are also considered significant over the last hundred years, placing strain on this naturally dynamic system. This study helps reveal patterns of change in the humid highlands over the last 10,000 cal yr BP regarding vegetation variability, climatic shifts, the historical influence of fire, tortoise disturbance, and recent anthropogenic impacts on the island. © 2012 Elsevier Ltd

When is an invasive not an invasive? Macrofossil evidence of doubtful native plant species in the Galápagos Islands

The Galápagos Islands are globally renowned for their ecological value and as a world symbol of scientific discovery; however the native biodiversity of this unique region is currently under threat. One of the primary concerns is the detrimental impact of approximately 750 nonnative plants introduced over the last 500 years of human presence in the archipelago. In addition to these known introduced species, there are an additional 62 vascular plants classified as "doubtful natives," where native status remains unclear. To help address the questions of provenance regarding these doubtfully native species and their impact on highland ecosystems over the past 500-1000 years, we analyzed plant macrofossils in sedimentary records. Appropriate species classification (native or introduced) was determined using baseline data of species presence on the islands. We confirmed that six plants (Ageratum conyzoides, Solanum americanum, Ranunculus flagelliformis, Brickellia diffusa, Galium canescens, and Anthephora hermaphrodita) once considered doubtful natives or introduced are actually native to the Galápagos flora. These results have relevance not just for the Galápagos but also many other oceanic islands in demonstrating the application of palaeobotanical data to conserving and restoring native biodiversity

Ensuring the long-term conservation of island ecosystems: why conservation biology needs palaeoecology

The discovery and colonization of islands by humans has invariably resulted in their widespread ecological transformation. The small and isolated populations of many island taxa, and their evolution in the absence of humans and their introduced taxa, mean that they are particularly vulnerable to human activities. Consequently, even the most degraded islands are a focus for restoration, eradication, and monitoring programmes to protect the remaining endemic and/or relict populations. Here, we build a framework that incorporates an assessment of the degree of change from multiple baseline reference periods using long-term ecological data. The use of multiple reference points may provide information on both the variability of natural systems and responses to successive waves of cultural transformation of island ecosystems, involving, for example, the alteration of fire and grazing regimes and the introduction of non-native species. We provide exemplification of how such approaches can provide valuable information for biodiversity conservation managers of island ecosystems