Tuberous Sclerosis Complex 1 Regulates dE2F1 Expression during Development and Cooperates with RBF1 to Control Proliferation and Survival

Previous studies in Drosophila melanogaster have demonstrated that many tumor suppressor pathways impinge on Rb/E2F to regulate proliferation and survival. Here, we report that Tuberous Sclerosis Complex 1 (TSC1), a well-established tumor suppressor that regulates cell size, is an important regulator of dE2F1 during development. In eye imaginal discs, the loss of tsc1 cooperates with rbf1 mutations to promote ectopic S-phase and cell death. This cooperative effect between tsc1 and rbf1 mutations can be explained, at least in part, by the observation that TSC1 post-transcriptionally regulates dE2F1 expression. Clonal analysis revealed that the protein level of dE2F1 is increased in tsc1 or tsc2 mutant cells and conversely decreased in rheb or dTor mutant cells. Interestingly, while s6k mutations have no effect on dE2F1 expression in the wild-type background, S6k is absolutely required for the increase of dE2F1 expression in tsc2 mutant cells. The canonical TSC/Rheb/Tor/S6k pathway is also an important determinant of dE2F1-dependent cell death, since rheb or s6k mutations suppress the developmentally regulated cell death observed in rbf1 mutant eye discs. Our results provide evidence to suggest that dE2F1 is an important cell cycle regulator that translates the growth-promoting signal downstream of the TSC/Rheb/Tor/S6k pathway

Laser Interferometers as Dark Matter Detectors

While the global cosmological and local galactic abundance of dark matter is well established, its identity, physical size, and composition remain a mystery. In this paper, we analyze an important question of dark matter detectability through its gravitational interaction, using current and next generation gravitational-wave observatories to look for macroscopic (kilogram-scale or larger) objects. Keeping the size of the dark matter objects to be smaller than the physical dimensions of the detectors, and keeping their mass as a free parameter, we derive the expected event rates. For favorable choice of mass, we find that dark matter interactions could be detected in space-based detectors such as LISA at a rate of one per ten years. We then assume the existence of an additional Yukawa force between dark matter and regular matter. By choosing the range of the force to be comparable to the size of the detectors, we derive the levels of sensitivity to such a new force, which exceeds the sensitivity of other probes in a wide range of parameters. For sufficiently large Yukawa coupling strength, the rate of dark matter events can then exceed 10 per year for both ground- and space-based detectors. Thus, gravitational-wave observatories can make an important contribution to a global effort of searching for nongravitational interactions of dark matter

Laser action in conducting polymers

We discuss both cooperative radiation and stimulated emission and consider their role in spectral narrowing of luminescent conducting polymers. We argue that cooperative radiation is favored in films with poor optical confinement. On the other hand, directional stimulated emission can be observed in dilute solution and thin films with superior optical confinement. Spectral narrowing in this case can be achieved by increasing either the excitation length or excitation intensity. The optical gain and loss coefficients are measured. Narrow line (approximately 1.5 cm-1) laser emission is observed in cylindrical microcavities formed by thin polymer films coated around glass fibers in the red and green spectral ranges. The cavity quality factors of these plastic lasers are mainly determined by selfabsorption and estimated to be about 5000.Optical Scnce, Engineering and Instrumentation '97, 1997, San Diego, CA, United StatesSergey V. Frolov, Maxim N. Shkunov, Z. Valy Vardeny, Masanori Ozaki, and Katsumi Yoshino "Laser action in conducting polymers", Proc. SPIE 3145, Optical Probes of Conjugated Polymers, (1 December 1997). DOI: https://doi.org/10.1117/12.29552

Vinculin gene is non-essential in Drosophila melanogaster

AbstractVinculin is thought to be an important cytoskeletal protein in the linkage between actin cytoskeleton and integrin transmembrane receptors. We identified Vinculin (Vinc) gene in the X chromosome of D. melanogaster. Drosophila vinculin is highly homologous in its N- and C-terminal domains both to mammalian and nematode vinculins, and contains internal repeats and proline-rich region typical for vinculins. The X chromosome rearrangement In(1LR)pn2a was found to disrupt Vinc so that the coding sequence is interrupted by the (AAGAG)n satellite DNA. Northern analysis revealed that the Vinc transcript is completely absent in the In(1LR)pn2a homozygous flies. Surprisingly, these Vinc flies are viable and fertile. This finding highlights plasticity and adaptive capacity of cellular cytoskeletal and anchorage system

A theory of thin shells with orbiting constituents

The self-gravitating, spherically symmetric thin shells built of orbiting particles are sstudied. Two new features are found. One is the minimal possible value for an angular momentum of particles, above which elleptic orbits become possible. The second is the coexistence of both the wormhole solutions and the elleptic or hyperbolic orbits for the same values of the parameters (but different initial conditions). Possible applications of these results to astrophysics and quantum black holes are briefly discussed.Comment: 22 pages, Latex, 10 eps figures. CERN preprint no. CERN-TH 2000-16

Stimulated emission and lasing in π-conjugated polymer films, microstructures and opal photonic crystals

SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, 1999, Denver, CO, United StatesZ. Valy Vardeny, Sergey V. Frolov, Douglas Chinn, Maxim N. Shkunov, Werner Gellermann, Katsumi Yoshino, Akihiko Fujii, Richard V. Gregory, Ray H. Baughman, and Anvar A. Zakhidov "Stimulated emission and lasing in π-conjugated polymer films, microstructures, and opal photonic crystals", Proc. SPIE 3797, Organic Light-Emitting Materials and Devices III, (17 December 1999). DOI: https://doi.org/10.1117/12.37269

Native and graphene-coated flat and stepped surfaces of TiC

Titanium carbide attracts growing interest as a substrate for graphene growth and as a component of the composite carbon materials for supercapacitors, an electrode material for metal-air batteries. For all these applications, the surface chemistry of titanium carbide is highly relevant and being, however, insufficiently explored especially at atomic level is a subject of our studies. Applying X-ray photoelectron spectroscopy (XPS) to clean (111) and (755) surfaces of TiC, we were able to obtain the detailed spectroscopic pattern containing information on the plasmon structure, shake up satellite, the peak asymmetry and, finally, surface core level shift (SCLS) in C 1s spectra. The latter is essential for further precise studies of chemical reactions. Later on, we studied interface between TiC (111) and (755) and graphene and found the SCLS variation due to strong chemical interaction between graphene and substrate. This interaction is also reflected in the peculiar band structure of graphene probed by angle-resolved photoelectron spectroscopy (ARPES). Based on LEED data the structure is close to (7√3 × 7√3)R30°, with graphene being slightly corrugated. We found that similarly to the graphene on metals, the chemical interaction between graphene and TiC can be weakened by means of intercalation of oxygen atoms underneath graphene.We thank Helmholtz-Zentrum Berlin (HZB) for the allocation of synchrotron radiation beamtimes at the Russian-German and UE112-PGM2 beamlines. The work was financially supported by the Russian Science Foundation (project 16-42-01093). DFT calculations were performed at “Lomonosov” MSU supercomputer.Peer reviewe

Context-Dependent Requirement for dE2F during Oncogenic Proliferation

The Hippo pathway negatively regulates the cell number in epithelial tissue. Upon its inactivation, an excess of cells is produced. These additional cells are generated from an increased rate of cell division, followed by inappropriate proliferation of cells that have failed to exit the cell cycle. We analyzed the consequence of inactivation of the entire E2F family of transcription factors in these two settings. In Drosophila, there is a single activator, dE2F1, and a single repressor, dE2F2, which act antagonistically to each other during development. While the loss of the activator dE2F1 results in a severe impairment in cell proliferation, this defect is rescued by the simultaneous loss of the repressor dE2F2, as cell proliferation occurs relatively normally in the absence of both dE2F proteins. We found that the combined inactivation of dE2F1 and dE2F2 had no significant effect on the increased rate of cell division of Hippo pathway mutant cells. In striking contrast, inappropriate proliferation of cells that failed to exit the cell cycle was efficiently blocked. Furthermore, our data suggest that such inappropriate proliferation was primarily dependent on the activator, de2f1, as loss of de2f2 was inconsequential. Consistently, Hippo pathway mutant cells had elevated E2F activity and induced dE2F1 expression at a point when wild-type cells normally exit the cell cycle. Thus, we uncovered a critical requirement for the dE2F family during inappropriate proliferation of Hippo pathway mutant cells

Fast calculation of thermodynamic and structural parameters of solutions using the 3DRISM model and the multi-grid method

In the paper a new method to solve the tree-dimensional reference interaction site model (3DRISM) integral equations is proposed. The algorithm uses the multi-grid technique which allows to decrease the computational expanses. 3DRISM calculations for aqueous solutions of four compounds (argon, water, methane, methanol) on the different grids are performed in order to determine a dependence of the computational error on the parameters of the grid. It is shown that calculations on the grid with the step 0.05\Angstr and buffer 8\Angstr give the error of solvation free energy calculations less than 0.3 kcal/mol which is comparable to the accuracy of the experimental measurements. The performance of the algorithm is tested. It is shown that the proposed algorithm is in average more than 12 times faster than the standard Picard direct iteration method.Comment: the information in this preprint is not up to date. Since the first publication of the preprint (9 Nov 2011) the algorithm was modified which allowed to achieve better results. For the new algorithm see the JCTC paper: DOI: 10.1021/ct200815v, http://pubs.acs.org/doi/abs/10.1021/ct200815