Hydrothermal thresholds for seed germination in winter annual forbs from old-field Mediterranean landscapes

Under Mediterranean climates with dry‐hot summers and cool‐wet winters, many forbs with potential for habitat restoration are winter annuals, but there is little information about their germination. We performed laboratory germination experiments on 13 ruderal dicots native to Andalusia (southern Spain). We measured the germination of recently harvested seeds from natural populations across nine temperature treatments (from 5 to 35 °C, constant and alternate); two storage periods; and eight water stress treatments (from 0 to −1.0 MPa). We then calculated the hydrothermal thresholds for seed germination. Final germination ranged from 0–100% and results were mixed in response to temperature. Base temperature was below 6 °C, optimal temperature was around 14 °C and the ceiling temperature around 23 °C. For five species, 10 months of storage improved total germination, indicating a dormancy‐breaking effect, but the other species did not respond or had their germination reduced. All species were relatively tolerant to water stress, with base water potential ranging from −0.8 to −1.8 MPa. Our results suggest that hydrothermal germination thresholds, rather than physiological dormancy, are the main drivers of germination phenology in annual forbs from Mediterranean semi‐dry environments. The variation in germination responses of these forb species differs from winter annual grasses, but their seeds are all suitable for being stored before restoration.European Union's Seventh Framework Programme FP7/2007‐2013/. Grant Number: n°607785 Government of Asturias European Commission. Grant Number: ACA14‐1

Attraction of green lacewings (Neuroptera: Chrysopidae) to native plants used as ground cover in woody Mediterranean agroecosystems

Using native seeds to establish semi-natural habitats is a novel strategy to restore biodiversity and ecosystem services such as biological control. As green lacewings (Neuroptera: Chrysopidae) are regarded as major biological control agents in different crops, the objective of this study was to test the attractiveness of selected native plant species to lacewings. During a two-year (2016–2017) field experiment near Villarrubia (Andalusia, Spain), 42 native plant species belonging to 13 families were planted. Plant development was monitored, and arthropods were vacuumed from each plot twice during the peak May flowering period. Green lacewings were observed to use 28 of the 36 well developed species to reproduce, feed and/or rest. Chrysoperla lucasina was the predominant species among the captured adults. No clear pattern of attraction common to all species was observed during the two-year sampling period. In 2017, eight plant species showed above-average chrysopid abundance. In addition, Acari abundance correlated positively with chrysopid abundance. Given the attraction of chrysopids, the effect of pests and disease on olive orchards and satisfactory plant development, we consider Biscutella auriculata, Borago officinalis, Silene colorata, Crepis capillaris, Nigella damascena and Papaver rhoeas to be the native plant species best suited to host chrysopids and to restore ground cover in perennial Mediterranean crops.We wish to thank Joaquin Chocano, Luis Plaza and María Luisa Fernández for their field and laboratory assistance, Belen Cotes for assisting with statistical data, Víctor Monserrat for his advice on taxonomy and Michael O’Shea for proofreading the manuscript. The study was funded by the Junta de Andalucía (project P12-AGR-1419), CSIC (project 201540E007), Marie Curie ITN (FP7/2007-2013/n°607785) and Semillas Silvestres S.L

Hydrothermal thresholds for seed germination in winter annual forbs from old‐field Mediterranean landscapes

Under Mediterranean climates with dry‐hot summers and cool‐wet winters, many forbs with potential for habitat restoration are winter annuals, but there is little information about their germination. We performed laboratory germination experiments on 13 ruderal dicots native to Andalusia (southern Spain). We measured the germination of recently harvested seeds from natural populations across nine temperature treatments (from 5 to 35 °C, constant and alternate); two storage periods; and eight water stress treatments (from 0 to −1.0 MPa). We then calculated the hydrothermal thresholds for seed germination. Final germination ranged from 0–100% and results were mixed in response to temperature. Base temperature was below 6 °C, optimal temperature was around 14 °C and the ceiling temperature around 23 °C. For five species, 10 months of storage improved total germination, indicating a dormancy‐breaking effect, but the other species did not respond or had their germination reduced. All species were relatively tolerant to water stress, with base water potential ranging from −0.8 to −1.8 MPa. Our results suggest that hydrothermal germination thresholds, rather than physiological dormancy, are the main drivers of germination phenology in annual forbs from Mediterranean semi‐dry environments. The variation in germination responses of these forb species differs from winter annual grasses, but their seeds are all suitable for being stored before restoration.European Union's Seventh Framework Programme FP7/2007‐2013/. Grant Number: n°607785 Government of Asturias European Commission. Grant Number: ACA14‐1

International principles and standards for native seeds in ecological restoration

© 2020 The Authors. Restoration Ecology published by Wiley Periodicals LLC on behalf of Society for Ecological Restoration. The growing demand for native seeds in ecological restoration and rehabilitation, whether for mining, forest, or ecosystem restoration, has resulted in a major global industry in the sourcing, supply, and sale of native seeds. However, there are no international guidance documents for ensuring that native seeds have the same standards of quality assurance that are regular practice in the crop and horticultural industries. Using the International Principles and Standards for the Practice of Ecological Restoration as a foundation document, we provide for the first time a synthesis of general practices in the native seed supply chain to derive the Principles and Standards for Native Seeds in Ecological Restoration (“Standards”). These practices and the underpinning science provide the basis for developing quality measures and guidance statements that are adaptable at the local, biome, or national scale. Importantly, these Standards define what is considered native seed in ecological restoration and highlight the differences between native seeds versus seeds of improved genetics. Seed testing approaches are provided within a logical framework that outline the many different dormancy states in native seed that can confound restoration outcomes. A “pro-forma” template for a production label is included as a practical tool that can be customized for local needs and to standardize reporting to end-users on the level of seed quality and germinability to be expected in a native seed batch. These Standards are not intended to be mandatory; however, the guidance statements provide the foundation upon which regulatory approaches can be developed by constituencies and jurisdictions

DataSheet_1_Seed sourcing strategies for ecological restoration under climate change: A review of the current literature.docx

Climate change continues to alter the seasonal timing and extremes of global temperature and precipitation patterns. These departures from historic conditions along with the predicted variability of future climates present a challenge to seed sourcing, or provenance strategy decisions, within the practice of ecological restoration. The “local is best” for seed sourcing paradigm is predicated upon the assumption that ecotypes are genetically adapted to their local environment. However, local adaptations are potentially being outpaced by climate change, and the ability of plant populations to naturally migrate or shift their distribution accordingly may be limited by habitat fragmentation. Restoration practitioners and natural area managers have a general understanding of the importance of matching the inherent adaptations of source populations with the current and/or future site conditions where those seeds or propagules are planted. However, for many species used in seed-based restoration, there is a lack of empirical evidence to guide seed sourcing decisions, which are critical for the longevity and ecological function of restored natural communities. With the goal of characterizing, synthesizing, and applying experimental research to guide restoration practice, we conducted a systematic review of the literature on provenance testing of taxa undertaken to inform seed sourcing strategies for climate resiliency. We found a strong bias in the choice of study organism: most studies have been conducted on tree species. We also found a strong bias regarding where this research has been conducted, with North America (52%) and Europe (31%) overrepresented. Experiments were designed to assess how propagule origin influences performance across both climatic (26%) and geographic (15%) distance, with some studies focused on determining how climate normal conditions (39%) impacted performance related to survivorship, growth and other parameters. We describe the patterns and gaps our review identified, highlight specific topics which require further research, and provide practical suggestions of immediate and longer-term tools that restoration practitioners can use to guide and build resilient natural communities under future climate scenarios.</p

Table_2_Seed sourcing strategies for ecological restoration under climate change: A review of the current literature.xlsx

Climate change continues to alter the seasonal timing and extremes of global temperature and precipitation patterns. These departures from historic conditions along with the predicted variability of future climates present a challenge to seed sourcing, or provenance strategy decisions, within the practice of ecological restoration. The “local is best” for seed sourcing paradigm is predicated upon the assumption that ecotypes are genetically adapted to their local environment. However, local adaptations are potentially being outpaced by climate change, and the ability of plant populations to naturally migrate or shift their distribution accordingly may be limited by habitat fragmentation. Restoration practitioners and natural area managers have a general understanding of the importance of matching the inherent adaptations of source populations with the current and/or future site conditions where those seeds or propagules are planted. However, for many species used in seed-based restoration, there is a lack of empirical evidence to guide seed sourcing decisions, which are critical for the longevity and ecological function of restored natural communities. With the goal of characterizing, synthesizing, and applying experimental research to guide restoration practice, we conducted a systematic review of the literature on provenance testing of taxa undertaken to inform seed sourcing strategies for climate resiliency. We found a strong bias in the choice of study organism: most studies have been conducted on tree species. We also found a strong bias regarding where this research has been conducted, with North America (52%) and Europe (31%) overrepresented. Experiments were designed to assess how propagule origin influences performance across both climatic (26%) and geographic (15%) distance, with some studies focused on determining how climate normal conditions (39%) impacted performance related to survivorship, growth and other parameters. We describe the patterns and gaps our review identified, highlight specific topics which require further research, and provide practical suggestions of immediate and longer-term tools that restoration practitioners can use to guide and build resilient natural communities under future climate scenarios.</p

Table_1_Seed sourcing strategies for ecological restoration under climate change: A review of the current literature.xlsx

Climate change continues to alter the seasonal timing and extremes of global temperature and precipitation patterns. These departures from historic conditions along with the predicted variability of future climates present a challenge to seed sourcing, or provenance strategy decisions, within the practice of ecological restoration. The “local is best” for seed sourcing paradigm is predicated upon the assumption that ecotypes are genetically adapted to their local environment. However, local adaptations are potentially being outpaced by climate change, and the ability of plant populations to naturally migrate or shift their distribution accordingly may be limited by habitat fragmentation. Restoration practitioners and natural area managers have a general understanding of the importance of matching the inherent adaptations of source populations with the current and/or future site conditions where those seeds or propagules are planted. However, for many species used in seed-based restoration, there is a lack of empirical evidence to guide seed sourcing decisions, which are critical for the longevity and ecological function of restored natural communities. With the goal of characterizing, synthesizing, and applying experimental research to guide restoration practice, we conducted a systematic review of the literature on provenance testing of taxa undertaken to inform seed sourcing strategies for climate resiliency. We found a strong bias in the choice of study organism: most studies have been conducted on tree species. We also found a strong bias regarding where this research has been conducted, with North America (52%) and Europe (31%) overrepresented. Experiments were designed to assess how propagule origin influences performance across both climatic (26%) and geographic (15%) distance, with some studies focused on determining how climate normal conditions (39%) impacted performance related to survivorship, growth and other parameters. We describe the patterns and gaps our review identified, highlight specific topics which require further research, and provide practical suggestions of immediate and longer-term tools that restoration practitioners can use to guide and build resilient natural communities under future climate scenarios.</p

Collection and production of native seeds for ecological restoration

© 2020 The Authors. Restoration Ecology published by Wiley Periodicals LLC on behalf of Society for Ecological Restoration The global push to achieve ecosystem restoration targets has resulted in an increased demand for native seeds that current production systems are not able to fulfill. In many countries, seeds used in ecological restoration are often sourced from natural populations. Though providing seed that is reflective of the genetic diversity of a species, wild harvesting often cannot meet the demands for large-scale restoration and may also result in depletion of native seed resources through over harvesting. To improve seed production and decrease seed costs, seed production systems have been established in several countries to generate native seeds based on agricultural or horticultural production methods or by managing natural populations. However, there is a need to expand these production systems which have a primary focus on herbaceous species to also include slower maturing shrub and tree seed. Here we propose that to reduce the threat of overharvest on the viability of natural populations, seed collection from natural populations should be replaced or supplemented by seed production systems. This overview of seed production systems demonstrates how to maximize production and minimize unintended selection bias so that native seed batches maintain genetic diversity and adaptability to underpin the success of ecological restoration programs

Dormancy and germination: making every seed count in restoration

From 50 to 90% of wild plant species worldwide produce seeds that are dormant upon maturity, with specific dormancy traits driven by species' occurrence geography, growth form, and genetic factors. While dormancy is a beneficial adaptation for intact natural systems, it can limit plant recruitment in restoration scenarios because seeds may take several seasons to lose dormancy and consequently show low or erratic germination. During this time, seed predation, weed competition, soil erosion, and seed viability loss can lead to plant re-establishment failure. Understanding and considering seed dormancy and germination traits in restoration planning are thus critical to ensuring effective seed management and seed use efficiency. There are five known dormancy classes (physiological, physical, combinational, morphological, and morphophysiological), each requiring specific cues to alleviate dormancy and enable germination. The dormancy status of a seed can be determined through a series of simple steps that account for initial seed quality and assess germination across a range of environmental conditions. In this article, we outline the steps of the dormancy classification process and the various corresponding methodologies for ex situ dormancy alleviation. We also highlight the importance of record-keeping and reporting of seed accession information (e.g. geographic coordinates of the seed collection location, cleaning and quality information, storage conditions, and dormancy testing data) to ensure that these factors are adequately considered in restoration planning