More Legumes for Green Manure = More Corn

Iowa State College experiments show that a lot of nitrogren can be cheaply produced by adding a legume seeding to the oats preceding corn. Research indicates every farmer could profitably make a legume seeding for green manure in every acre of oats in addition to meadow and rotation plantings

Reduce Tall Fescue Toxicosis in \u3cem\u3eFestuca arundinacea\u3c/em\u3e Forage Systems with Legumes

Tall fescue (Lolium arundinaceum = Festuca arundinacea) is an important cool-season grass because of persistence and spring and autumn productivity. Most existing tall fescue pastures in Tennessee are Kentucky-31 (KY-31) and are infested with the endophytic fungus Neotyphodium coenophialum that causes tall fescue toxicosis. Symptoms in beef cattle include reduced rates of gain, poor conception rates, decreased dry matter intake, a long rough haircoat in summer, and very low serum prolactin. Earlier studies indicated that the performance of cattle grazing endophyte-free (EF) tall fescue is superior to that of cattle grazing endophyte-infected (EI) tall fescue (Fribourg et al., 1995). However, EF tall fescue is not as persistent as EI. Addition of clovers to tall fescue pastures is a recommended practice. Our objective was to compare forage quality, in vitro dry matter disappearance, dry matter intake, and animal performance of EI and EF KY-31 pastures, and inclusion of legumes

Orchardgrass Pastures for Early-Weaned Beef Calves

Orchardgrass (Dactylis glomerata) grown alone or with ladino white (Trifolium repens) and red (T. pratense) clovers, provides a high quality March to June pasture. However, many beef producers have their cows calving in January-March, thus producing offspring that are too young to utilize spring forage well. As an alternative, some producers have their cows bred so that calves are born in autumn (September-November). By spring, these calves are old enough to consume pasture forage. The few earlier studies on early weaning of fall-born calves consist of drylot feeding of high quality hay or concentrates. This study evaluated the potential of orchardgrass and orchardgrass clover pastures to meet the nutritional needs of early-weaned fall-born beef calves (Bos taurus). Fallborn steers, 4.5-mo old, weighing about 144 kg, were used in each of 3 yr. Twenty-four were weaned and allotted at random to one of four duplicate pasture treatments: (1) TN-Syn-2 orchardgrass, grown alone or (2) with ladino white and red clovers ; (3) ‘Benchmark’ orchardgrass, grown alone or (4) with the clovers; another 24 remained with their dams. Early-weaned calves on high quality pastures performed well, with daily gains between 640 and 925 g d-1. Dams which were not suckled were in better body condition going into the summer than those with calves

MIXED MODELS COMBINED ANALYSIS OF INDEPENDENT GRAZING TRIALS

The mixed models procedure (MMP) was used to analyze pooled data sets from 12 independent studies over 13 yr at 9 locations in 7 states to provide combined estimates of daily gains by beef steers grazing tall fescue pastures with different levels of infestation by Acremonium coenophialum, with and without clover. Spring, summer, and combined spring + summer data were analyzed separately. The MMP permitted estimation of the fixed effects of treatments over a broad inference space of future years and different tall fescue pastures over a wide geographic range, detected some relationships not apparent in the individual studies, and provided a more coherent body of information than do the results obtained from each discrete study. Logistical and financial constraints that force undesirable compromises in the conduct of future grazing and other expensive or time-consuming research may be avoided by using MMP within the context of cooperative projects

Grazing Endophyte Infested Tall Fescue and Changes in Bovine Blood Components and Gain

Tall fescue (Festuca arundinacea) is utilized on more than 15 million ha of pasture in the eastern USA and over 80% of the plants in this area are infested with the endophyte Neotyphodium coenophialum. Tall fescue toxicosis results from consumption of the infested (E+) grass host and is an important problem that causes considerable economic loss to producers. However, the agronomic attributes of tall fescue make it an attractive forage because of its ability to withstand cool temperatures, drought, poor soil conditions and intensive defoliation. There is need to understand mechanisms of animal physiology that are affected by the alkaloids produced by the endophyte/grass association. Blood serum samples were collected from steers (Bos taurus) grazing either E+ or endophyte free (E-) tall fescue in spring and summer during three consecutive years and were related to animal performance. Consistent and significant changes associated with E+ tall fescue were noted for daily gain, prolactin, albumin/globulin ratio, alanine aminotransferase, cholesterol, creatinine, globulin, total bilirubin, total serum protein, copper, red blood cells, mean corpuscular volume, mean corpuscular hemoglobin, and eosinophils. Thus, repeatable changes that occur in serum biochemical and blood cellular values of cattle grazing E+ tall fescue were identified and will aid in understanding the pathogenesis of the toxicosis

The hRPC62 subunit of human RNA polymerase III displays helicase activity.

In Eukaryotes, tRNAs, 5S RNA and U6 RNA are transcribed by RNA polymerase (Pol) III. Human Pol III is composed of 17 subunits. Three specific Pol III subunits form a stable ternary subcomplex (RPC62-RPC39-RPC32α/β) being involved in pre-initiation complex formation. No paralogues for subunits of this subcomplex subunits have been found in Pols I or II, but hRPC62 was shown to be structurally related to the general Pol II transcription factor hTFIIEα. Here we show that these structural homologies extend to functional similarities. hRPC62 as well as hTFIIEα possess intrinsic ATP-dependent 3'-5' DNA unwinding activity. The ATPase activities of both proteins are stimulated by single-stranded DNA. Moreover, the eWH domain of hTFIIEα can replace the first eWH (eWH1) domain of hRPC62 in ATPase and DNA unwinding assays. Our results identify intrinsic enzymatic activities in hRPC62 and hTFIIEα

Forward Analysis and Model Checking for Trace Bounded WSTS

We investigate a subclass of well-structured transition systems (WSTS), the bounded---in the sense of Ginsburg and Spanier (Trans. AMS 1964)---complete deterministic ones, which we claim provide an adequate basis for the study of forward analyses as developed by Finkel and Goubault-Larrecq (Logic. Meth. Comput. Sci. 2012). Indeed, we prove that, unlike other conditions considered previously for the termination of forward analysis, boundedness is decidable. Boundedness turns out to be a valuable restriction for WSTS verification, as we show that it further allows to decide all $\omega$-regular properties on the set of infinite traces of the system

MLN51 Stimulates the RNA-Helicase Activity of eIF4AIII

The core of the exon-junction complex consists of Y14, Magoh, MLN51 and eIF4AIII, a DEAD-box RNA helicase. MLN51 stimulates the ATPase activity of eIF4AIII, whilst the Y14-Magoh complex inhibits it. We show that the MLN51-dependent stimulation increases both the affinity of eIF4AIII for ATP and the rate of enzyme turnover; the K (M) is decreased by an order of magnitude and k (cat) increases 30 fold. Y14-Magoh do inhibit the MLN51-stimulated ATPase activity, but not back to background levels. The ATP-bound form of the eIF4AIII-MLN51 complex has a 100-fold higher affinity for RNA than the unbound form and ATP hydrolysis reduces this affinity. MLN51 stimulates the RNA-helicase activity of eIF4AIII, suggesting that this activity may be functionally important