MOLECULAR IDENTIFICATION OF Liriomyza sp. IN THE NORTHEAST AND SOUTHEAST REGIONS OF BRAZIL

In Brazil, species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and Fabaceae. Studies suggest existence of a species complex within this genus, based on the presence of morphological similarities among the species Liriomyza trifolii (Burgess), L. sativae Blanchard and L. huidobrensis (Blanchard). The present study aimed to use DNA barcoding to establish new distribution records of L. sativae in distinct regions in Brazil, determine intra- and inter-population genetic diversity, and reconstruct the phylogeny of Liriomyza species using the DNA barcode sequences. Identity values were between 97% and 99%, confirming that all the examined Brazilian populations belonged to the species L. sativae. Phylogenetic analyses indicated the presence of a single clade of L. sativae, composed of seven populations. Intra-population analysis on individuals of these populations indicated low levels of nucleotide and haplotype diversity. The haplotype network indicated presence of only 14 haplotypes distributed among the Brazilian populations. The genetic similarities shared by the Brazilian populations of L. sativae suggest that these populations are closely related. Genetic patterns observed among populations of L. sativae might be associated with bottleneck events or founder effect during establishment of this leafminer in Brazil

Analysis of the genetic structure of allopatric populations of Lutzomyia umbratilis using the period clock gene

Submitted by Adagilson Silva ([email protected]) on 2017-09-13T13:47:34Z No. of bitstreams: 1 26655040 2015 fre-ana.oa.pdf: 1632788 bytes, checksum: 112764016653875cf14c2bbb5487d1aa (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-09-13T17:23:45Z (GMT) No. of bitstreams: 1 26655040 2015 fre-ana.oa.pdf: 1632788 bytes, checksum: 112764016653875cf14c2bbb5487d1aa (MD5)Made available in DSpace on 2017-09-13T17:23:45Z (GMT). No. of bitstreams: 1 26655040 2015 fre-ana.oa.pdf: 1632788 bytes, checksum: 112764016653875cf14c2bbb5487d1aa (MD5) Previous issue date: 2016-02Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Oswaldo Cruz Foundation. Instituto Leônidas e Maria Deane. Laboratory of Infectious Disease Ecology in the Amazon. Manaus, AM, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Oswaldo Cruz Foundation. Research Center Aggeu Magalhaes. Departament of Microbiology. Recife, PE, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Oswaldo Cruz Foundation. Instituto Leônidas e Maria Deane. Laboratory of Infectious Disease Ecology in the Amazon. Manaus, AM, Brazil.In South America, Lutzomyia umbratilis is the main vector of Leishmania guyanensis, one of the species involved in the transmission of American tegumentary leishmaniasis. In Brazil, L. umbratilis has been recorded in the Amazon region, and an isolated population has been identified in the state of Pernambuco, Northeastern region. This study assessed the phylogeographic structure of three allopatric Brazilian populations of L. umbratilis. Samples of L. umbratilis were collected from Rio Preto da Eva (north of the Amazon River, Amazonas), from Manacapuru (south of the Amazon River), and from the isolated population in Recife, Pernambuco state. These samples were processed to obtain sequences of the period gene. Phylogenetic analysis revealed the presence of two distinct monophyletic clades: one clade comprised of the Recife and Rio Preto da Eva samples, and one clade comprised of the Manacapuru samples. Comparing the Manacapuru population with the Recife and Rio Preto da Eva populations revealed high indices of interpopulational divergence. Phylogenetic analysis indicated that geographical distance and environmental differences have not modified the ancestral relationship shared by the Recife and Rio Preto da Eva populations. Genetic similarities suggest that, in evolutionary terms, these populations are more closely related to each other than to the Manacapuru population. These results confirm the existence of an L. umbratilis species complex composed of at least two incipient species

Phenotypic and genotypic variations among three allopatric populations of Lutzomyia umbratilis, main vector of Leishmania guyanensis

Submitted by Adagilson Silva ([email protected]) on 2017-09-20T14:04:18Z No. of bitstreams: 1 26338469 2015 fre-phe.oa.pdf: 2163638 bytes, checksum: d8623ba27d611eec48f95113299aba1e (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-09-20T14:12:14Z (GMT) No. of bitstreams: 1 26338469 2015 fre-phe.oa.pdf: 2163638 bytes, checksum: d8623ba27d611eec48f95113299aba1e (MD5)Made available in DSpace on 2017-09-20T14:12:14Z (GMT). No. of bitstreams: 1 26338469 2015 fre-phe.oa.pdf: 2163638 bytes, checksum: d8623ba27d611eec48f95113299aba1e (MD5) Previous issue date: 2015-09-04Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilIn South America, Lutzomyia umbratilis is the main vector of Leishmania guyanensis, one of the species involved in the transmission of American tegumentary leishmaniasis. In Brazil, L. umbratilis has been recorded in the Amazon region, and in the state of Pernambuco, Northeastern region, where an isolated population has been identified. This study assessed the phylogeographic structure and size and shape differences of the wing of three Brazilian populations