Language And Design Practices: Some Usability Issues In The Context Of The Social Web [práticas De Linguagem E Design: Algumas Questões De Usabilidade No Contexto Da Web Social]

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(versão cd-rom)Escrita, L.M., (2010) Interlocução e Moderação em Um Fórum Online Do Orkut, p. 123. , Dissertação de Mestrado. Campinas: Instituto de Estudos da Linguagem, UNICAMPLuria, A.R., (1973) Fundamentos de Neuropsicologia, , Cultrix, São Paulo SPLuria, A.R., (1977) Neuropsychological Studies in Aphasia, , Swets & Zeitlinger B.V. AmsterdanMarcuschi, L.A., Gêneros textuais emergentes no contexto da tecnologia digital (2005) Hipertextos e Gêneros Digitais: Novas Formas de Construção Do Sentido, pp. 13-67. , Editora Lucerna, Rio de JaneiroNielsen, J., Heuristic evaluation (1994) Usability Inspection Methods, pp. 25-62. , John Wiley & Sons, Nova Iorque NYhttp://www.orkut.com.brRocha, H.V., O ambiente TelEduc para a educação a distância baseada na Web: princípios funcionalidades e perspectivas de desenvolvimento (2002) Educação A Distância: Fundamentos e Práticas, pp. 197-212. , UNICAMP/NIED, Campinas, SPRocha, H.V., Baranauskas, M.C.C., (2000) Design e Avaliação de Interfaces Humano-computador, , IME-USP São Paulo SPRojo, R.H.R., Letramentos digitais-a leitura como réplica ativa (2007) Trabalhos em Lingüística Aplicada, 46 (2), pp. 63-78Scolari, C.A., Hipertextos interfaces, interacciones (2004) DeSignis, 5, pp. 73-84. , Editorial GedisaSorj, B., Guedes, L.E., Exclusão digital-problemas conceituais, evidências empíricas e políticas públicas (2005) Novos Estudos CEBRAP, 72, pp. 101-117Teleduc-Ensino À Distância, , http://teleduc.nied.unicamp.br/Vygotsky, L.S., (1926) Psicologia Pedagógica, , Martins Fontes, São Paulo SPVygotsky, L.S., (1934) Pensamento e Linguagem, , Martins Fontes São Paulo SPVygotsky, L.S.A., (1962) Formação Social da Mente, , Martins Fontes, São Paulo SPDos Xavier, A.C.S., (2002) O Hipertexto Na Sociedade da Informação: A Constituição Do Modo de Enunciação Digital, , Tese de Doutorado. Campinas, SP: Campinas: Instituto de Estudos da Linguagem, UNICAM

Interaction Problems Accessing E-learning Environments In Multi-touch Mobile Devices: A Case Study In Teleduc

e-Learning environments offer content, such text, audio, video, animations, using the Web infrastructure and they are designed to users interacting with keyboard, mouse and a medium-sized screen. Mobile devices, such as smartphones and tablets, have enough computation power to render Web pages, allowing browsing the Internet and access e-Learning environments. But, their main interaction style is touching, bringing some problems, cosmetics or severe ones. To identify these problems and correct them to allow mobile users access the TelEduc, an e-Learning environment, we planned a user test case with four volunteers using smartphones for access it. The collected data was analyzed and some problems were identified, giving some insights about the problems and barriers multi-touch mobile users dealt and can be easily corrected to give them a better interaction experience. To have an e-Learning environment with good usability in mobile devices is a first step to have a virtual learning environment for sharing data between desktop and mobile devices and understand the behavior of mobile users in this context.199206(2013) IPhone 3GS-Technical Specifications, , http://support.apple.com/kb/SP565, Accessed in January 2013(2013) IOS 5, , http://developer.apple.com/library/ios/#releasenotes/General/ WhatsNewIniPhoneOS/Articles/iOS5.html, Accessed in January 2013Casany, J.M., Extending moodle services to mobile devices: The moodbile project (2012) Proceedings of the 6th International Conference on Mobile Ubiquitous Computing, Systems, Services and Technologies, pp. 24-28. , Barcelona, Spain(2013) CKEditor-WYSIWYG Text and HTML Editor for the Web, , http://www.ckeditor.com, CKSource-Frederico Knabben Accessed in January 2013Da Silva, A.C., Da Rocha, H.V., Improving e-learning environment with gpl software-the visualization content case for computers and mobile devices (2012) Proceedings of the 6th International Conference on Mobile Ubiquitous Computing, Systems, Services and Technologies, pp. 222-227. , Barcelona, SpainFranco, A.M., O ambiente virtual de aprendizagem e sua incorporao na Unicamp (2003) Educao e Pesquisa, 29 (2), pp. 341-353(2013) Google, , http://www.android.com/, Accessed in January 2013Kaikkonen, A., Full or tailored mobile web-where and how do people browse on their mobile? (2008) Proceedings of the International Conference on Mobile Technology, Applications and Systems, pp. 111-111. , I-Lan, TaiwanLave, J., Wenger, E., (1991) Situated Learning-Legitimate Peripheral Participation, , University Press, Cambrigde, USAMaurer, E.M., Mobile or desktop website? Website usage on multitouch devices (2007) Proceedings of the 6th Nordic Conference on Human-Computer Interaction, pp. 739-742. , Reykjavik, Iceland(2013) Moodle. Org: Open-source Community-based Tools for Learning, , http://moodle.org, Moodle Trust, Accessed in January 2013(2013) Moodle Trust Moodle App, , http://docs.moodle.org/dev/Mobile_app, AccessedinJanuary2013](2013) Milestone Android-Phone Without Compromise-Tech Specs-Motorola Mobility India, , http://www.motorola.com/Consumers/IN-EN/Consumer-Products-and-Services/ MobilePhones/ci.Motorola-MILESTONE-IN-EN.alt, Motorola Mobility Inc. Accessed in January 2013Normal, A.D., Nielsen, J., Gestural interfaces: A step backward in usability (2010) Interactions, 17 (5), pp. 46-49Papert, S., Constructionism: A new opportunity for elementary science education (1986) Proposal to the National Science Foundation, , Massachusetts, USA(2013) Sakai Project Collaboration and Learning-for Educators by Educators, , http://sakaiproject.org, SAKAI Environment, Accessed in January 2013Schmiedl, G., Mobile phone web browsing-a study on usage and usability of the mobile web (2009) Proceedings of the 11th International Conference on Human-Computer Interaction with Mobile Devices and Services, , Bonn, GermanyShrestha, S., Mobile web browsing: Usability study (2007) Proceedings of the 4th International Conference on Mobility Technology, Applications and Systems, pp. 187-194. , Singapore(2013) TelEduc Ensino Distia, , http://www.teleduc.org.br, AccessedinOctober2012] TelEduc EnvironmentValente, J.A., Computadores e conhecimento: Repensando a educao (1993) Gráfica da UNICAMP, , Campinas, BrazilValente, J.A., Formao de professores: Diferentes abordagens pedagógicas (1999) O Computador Na Sociedade Do Conhecimento, pp. 131-156. , Editor Valente, J. A. UNICAMP/NIED, Campinas, Brazi

Polyclonal Antibody-based ELISA in combination with specific PCR amplification of ITS 1 regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of 2 gummosis in cashew nut plants

Members of Botryosphaeriaceae family are associated with serious diseases in different plants 18 across the world. In cashew nut plants (Anacardium occidentale L.), the fungus Lasiodiplodia 19 theobromae causes a severe group of symptoms related to gummosis that results in decreased nut 20 production. The aim of this work was to develop an indirect enzyme-linked immunosorbent 21 assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in 22 planta (artificially and naturally infected) and to increase the detection specificity within the 23 fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A 24 collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in 25 rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis 26 anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal 27 material was employed in the ELISAs. The fungi ITS sequences were determined, and single 28 nucleotide polymorphisms were identified and used for primer design. For the naturally infected 29 2 plants, there was an approximately 4-fold variation in the absorbance values. Some positive 1 readings for asymptomatic samples were detected. For the artificially infected samples, an 2 ELISA-based weekly time-course analysis was conducted, and the values for samples from 0 and 3 7 days were lower than the threshold value. Beginning on day 14, the infection could be 4 detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the 5 experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, 6 but for Colletotrichum gloeosporioides, Phomopsis anacardii, Pestalotiopsis guepinii and 7 Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The 8 feasibility of ELISA as an early detection technique to assist in gummosis management was 9 demonstrated. PCR amplification based on ITS regions increases and complements serological 10 specificit

Monitoring cashew seedlings during interactions with the fungus Lasiodiplodia theobromae using chlorophyll fluorescence imaging

The chlorophyll (Chl) fluorescence imaging technique was applied to cashew seedlings inoculated with the fungus Lasiodiplodia theobromae to assess any disturbances in the photosynthetic apparatus of the plants before the onset of visual symptoms. Two-month-old cashew plants were inoculated with mycelium of L. theobromae isolate Lt19 or Lt32. Dark-adapted and light-acclimated whole plants or previously labelled, single, mature leaf from each plant were evaluated weekly for Chl fluorescence parameters. From 21 to 28 days, inoculation with both isolates resulted in the significantly lower maximal photochemical quantum yield of PSII (Fv/Fm) than those for control samples, decreasing from values of 0.78 to 0.62. In contrast, the time response of the measured fluorescence transient curve from dark-acclimated plants increased in both whole plants and single mature leaves in inoculated plants compared with controls. The Fv/Fm images clearly exhibited photosynthetic perturbations 14 days after inoculation before any visual symptoms appeared. Additionally, decays in the effective quantum yield of PSII photochemistry and photochemical quenching coefficient were also observed over time. However, nonphotochemical quenching increased during the evaluation period. We conclude that Fv/Fm images are the effective way of detecting early metabolic perturbations in the photosynthetic apparatus of cashew seedlings caused by gummosis in both whole plants and single leaves and could be potentially employed in larger-scale screening systems