6 research outputs found
Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats
Evaluation of the areas of neuronal cell bodies and nuclei in the myenteric plexus of the duodenum of adult rats
Morphoquantitative aspects of nitrergic myoenteric neurons from the stomach of diabetic rats supplemented with acetyl-L-carnitine
The NADPH-diaphorase (NADPH-d) positive myoenteric neurons from the body of the stomach of rats with streptozotocin-induced diabetes with or without supplementation with acetyl-L-carnitine (ALC) were evaluated. At the age of 105 days the animals were divided into four groups: normoglycaemic (C), normoglycaemic supplemented with ALC (CC), diabetic (D) and diabetic supplemented with ALC (DC). The supplementation with ALC (200 mg/kg body weight/day) to groups CC and DC was made during 105 days. After this period the animals were killed and the stomach removed and subjected to the histochemical technique of NADPH-d for the staining of the neurons of the myoenteric plexus. The area of 500 neurons of each group was investigated, as well as the neuronal density in an area of 23.84 mm(2) in each stomach. ALC promoted reduction (P < 0.05) of fasting glycaemia, water ingestion and areas of the profiles of the cell bodies of the NADPH-d neurons in the diabetic animals. The density of these neurons was not statistically different in the groups studied. It is suggested, therefore, a moderate neuroprotective effect of ALC, because the diminishment of the areas of the neuronal profiles in the supplemented diabetic animals, although being statistically significant relative to the non-supplemented diabetics, was not sufficient to equal the values from the non-diabetic controls
Morphoquantitative aspects of NADH-diaphorase myenteric neurons in the ileum of diabetic rats treated with acetyl-L-carnitine
In this work, we investigated the effect of the acetyl-L-carnitine (ALC) supplementation (200 mg/kg/day) on the myenteric neurons of the ileum of rats made diabetic by streptozotocin (35 mg/kg, i.v.). Four groups were used: diabetic (D), diabetic supplemented with ALC (DC), control (C) and control supplemented with ALC (CC). After 15 weeks of diabetes induction the animals were killed and the ileum was collected and subjected to whole-mount preparation to evidence the myenteric neurons through the histochemical technique of the NADH-diaphorase. The density of neurons seen in 12.72 min(2) of ileum showed no difference among the groups, although in group D it was 22% smaller than in group C, while group DC was 9% smaller to group CC. The profiles of the cell bodies (PC) of 1000 neurons per group were analysed. The neurons PC in group D decreased (P < 0.0001) when compared with other groups and increased (P < 0.0001) when compared with group DC. The incidence of neurons with a PC inferior to 200 mu m(2) was larger in group D. The frequency of neurons with a PC higher than 200 mu m(2) in group DC was close to those seen in groups C and CC. We concluded that ALC eases the loss of neurons and makes the incidence of myenteric neurons with a PC higher than 200 mu m(2) similar to the control rats
Efeitos da Reeducação Postural Global em escolares com escoliose Global Posture Reeducation effects in students with scoliosis
O objetivo deste estudo foi analisar o efeito do método da Reeducação Postural Global (RPG) em escolares com diagnóstico de escoliose torácica não estrutural (ETNE). Os escolares com indicativo de ETNE ao exame postural e teste de Adams negativo foram encaminhados ao exame radiográfico para comprovação diagnóstica. Foram selecionados 20 participantes (11 meninos e 9 meninas, com 10±3 anos), divididos randomicamente em dois grupos homogêneos: o que realizou o RPG (GRPG) durante 12 semanas com duração de 25 a 30 minutos cada sessão, de acordo com o que aguentou permanecer na postura; e o grupo controle (GC), sem intervenção. Após três meses, os dois grupos repetiram a avaliação postural e o exame radiográfico. Para avaliação das estatísticas, foi utilizada análise de variância (ANOVA) univariada, com medidas repetidas, seguida do Post Hoc de Tukey para identificar as possíveis diferenças intra e intergrupos. O valor de α foi de 0,05. O GRPG apresentou redução significativa no ângulo de Cobb na comparação intragrupo (Δ%=-35,100; p=0,009), mas o GC não (Δ%=9,520; p=0,789). Pode-se concluir que escolares submetidos ao método da RPG apresentaram melhora do quadro de escoliose torácica não estrutural.<br>The aim of this study was to analyze the effect of Global Reeducation Postural (RPG) method in scholars diagnosed with thoracic nonstructural scoliosis (ETNE). Scholars with indicative of ETNE to postural exam and Adams test negative were directed to radiographic exam to diagnostic. Twenty participants were selected (11 boys and 9 girls, 10±3 years old) and randomly divided in two homogeneous groups: group treated by RPG (GRPG) and control group (CG). The GRPG was submitted a RPG treatment during 12 weeks in sessions from 25 to 30 minutes in agreement with each student tolerance at posture. The CG didn't suffer intervention. After three months, both groups repeated the postural and radiographic exam. For statistics, it was used ANOVA univariated with repeated measures, followed by Tukey Post Hoc Test to identify the possible differences intra and intergroups. The α value used was 0.05. The GRPG present significant reduction at Cobb angle between pre and posttest (Δ%=-35.100; p=0.009), but the CG didn´t show (Δ%=9.520; p=0.789). It was concluded that scholars submitted to RPG method presented improvement in thoracic nonstructural scoliosis
Neurônios NADH-diaforase positivos do jejuno de ratos adultos (Rattus norvegicus) desnutridos: aspectos quantitativos NADH-diaphorase positive neurons of the jejunum of disnurtured adult rats (Rattus norvegicus): quantitative aspects
Temos por objetivo contribuir com informações sobre os aspectos quantitativos dos neurônios mioentéricos NADH-diaforase positivos do jejuno de ratos submetidos a desnutrição protéica. Foram utilizados 10 ratos (90 dias de idade), divididos em grupos: controle (n=5, ±278g) e desnutrido (n=5, ±280g). Nos 120 dias seguintes, os ratos do grupo controle receberam ração com teor protéico de 22%, os do grupo desnutrido, 8%. Ao final deste período, os ratos do grupo controle pesaram ±394,4g e os desnutrido ±273,5g.O jejuno foi submetido à técnica histoquímica da NADH-diaforase para evidenciação de células nervosas em preparado de membranas. Foram contados os neurônios presentes em 80 campos microscópicos em ambos os grupos. Verificaram-se no controle ±674,6 neurônios e no desnutrido ±1326,8 neurônios; A dieta não alterou a organização dos neurônios entretanto, levou a um menor desenvolvimento corporal nos animais desnutridos, contribuindo para que os neurônios destes sofressem menor dispersão e apresentassem maior densidade por mm².<br>We aim at contributing with information on the quantitative aspects of the NADH-diaphorase positive myenteric neurons of the jejunum of adult rats subjected to protein desnutrition. Ten rats aging 90 days were divided into two groups: control (n=5, ±278 g) and disnurtured (n=5, ±280 g). In the following 120 days, the rats from the control group had chow with 22% protein level, and those from the disnurtured group, with 8% protein level. After this period, the control rats weighted ±394.4g and the disnurtured ±273.5g. The jejunum was subjected to the histochemical technique of the NADH-diaphorase to stain nerve cells in whole-mounts. The neurons found in 80 microscopic fields of both groups were counted. In the control ±674.6 neurons were observed, and ±1326.8 neurons were counted in the disnurtured group. The low-protein diet did not alter the organization of the neurons, but led to a smaller body growth in the disnurtured animals, preventing neuronal dispersal and leading to a greater density per mm²