Production Of Monoglycerides And Diglycerides Through Lipase-catalyzed Glycerolysis And Molecular Distillation [produção De Monoacilgliceróis E Diacilgliceróis Via Glicerólise Enzimática E Destilação Molecular]

Monoacilglycerides and diacilglycerides are produced through lipase-catalyzed glycerolysis of soybean oil using Candida antárctica B in a solvent-free system. The reaction was carried out at a glycerol to triacylglycerol molar ratio of 8:1 with 2% of lipase. Acylglycerides, free fatty acids (FFA) and glycerol produced were separated employing the molecular distillation process. Starting from a product of enzymatic reaction 25.06% of triacylglycerols, 46.63% of diacylglycerides, 21.72% of monoacylglycerides, 5.38% of FFA and 1.21% of glycerol and after consecutively distillations, monoacylglycerides with 80% of purity was obtained and also oil with 54% of diacylglycerides to be used in human dietary.32615391543Meng, X., Zou, D., Shi, Z., Duan, Z., Mao, Z., (2006) Lipids, 39, p. 37Hickman, K.C.D., (1943) Chem. Rev., 34, p. 51Watanabe, H., Onizawa, K., Naito, S., Taguchi, H., Goto, N., Nagao, T., Matsuo, N., Itakura, H., (2001) Ann. Nutr. Metab., 45, p. 264Maki, C.K., Davidson, M., Tsushima, R., Matsuo, N., Tokimitsu, I., Umporowicz, D.N., Dicklin, M.R., Bell, M., (2002) Am. J. Clin. Nutr., 76, p. 1230Yasunaga, K., Glinsmann, W.H., Seo, Y., Katsuragi, Y., Kobayashi, S., Flickinger, B., Kennepohl, E., Borzelleca, J., (2004) Food Chem. Toxicol., 42, p. 1419Shimizu, M., Moriwaki, J., Nishide, T., Nakajima, Y., (2004) J. Am. Oil Chem. Soc., 81, p. 571Yamamoto, K., Takeshita, M., Tokimitsu, I., Watanabe, H., Mizuno, T., Asakawa, H., Tokunaga, K., Yagi, N., (2006) Nutrition, 22, p. 23Li, D., Xu, T., Takase, H., Tokimitsu, I., Zhang, P., Wang, Q., Yu, X., Zhang, A., (2008) Clinical Nutrition, 27, p. 203Sonntag, N.O.V., (1982) J. Am. Oil Chem. Soc., 59, p. 795Ferreira-Dias, S., Correia, A.C., Baptista, F.O., Fonseca, M.M.R., (2001) J. Mol. Catal. B: Enzym., 11, p. 699Guo, Z., Xu, X., (2006) Green Chem., 8, p. 54Micov, M., Lutisan, J., Cvengros, J., (1997) Sep. Sci. Technol., 32, p. 3051Batistella, C.B., Maciel, M.R.W., (1996) Comput. Chem. Eng., 20, p. 19Fregolente, L.V., Batistella, C.B., Maciel, R., Wolf-Maciel, M.R., (2005) J. Am. Oil Chem. Soc., 82, p. 673Batistella, C.B., Moraes, E.B., Maciel Filho, R., Maciel, M.R.W., (2002) Appl. Biochem. Biotechnol., 98, p. 1149Moraes, E.B., Batistella, C.B., Alvarez, M.E.T., Maciel Filho, R., Maciel, M.R.W., (2004) Appl. Biochem. Biotechnol., 113, p. 689Breivik, H., Haraldsson, G.G., Kristinsson, B., (1997) J. Am. Oil Chem. Soc., 74, p. 1425Kwon, S.J., Han, J.J., Rhee, J.S., (1995) Enzyme Microb. Technol., 17, p. 700Fregolente, P.B.L., Fregolente, L.V., Pinto, G.M.F., Batistella, C.B., Wolf-Maciel, M.R., Maciel Filho, R., (2008) Appl. Biochem. Biotechnol., 146, p. 165Schoenfelder, W., (2003) Eur. J. Lip. Sei. Technol., 105, p. 4