Physiological responses of asthmatics to endurance running

The aim of the study was to examine the physiological effect of endurance running on the asthmatic adult, an area in which there has been little research. The specific aims were to: firstly, examine the cardio-respiratory and metabolic responses to short term maximal and submaximal exercise of untrained asthmatics and non-asthmatics, and endurance-running-trained asthmatics; secondly, compare the physiological responses to prolonged endurance running of asthmatic and non-asthmatic athletes; thirdly, evaluate the effect of endurance running training on the previously untrained asthmatic. [Continues.

Designing open access, educational resources / Développer des ressources éducatives en libre accès

The recent bourgeoning of open educational resources has meant greater access to materials with open licenses in the public domain than ever before. Open educational resources are learning tools, such as textbooks, that are freely available and typically accessed online. Despite the expansion of open educational resources, many educators are still unacquainted with the nature and process of producing such resources. The purpose of this discussion paper is to share our experience in developing an open educational e-textbook for students in post-secondary programs for nursing and other health professions while highlighting practical tips for educators. The exemplar referenced in this paper focuses on vital signs’ measurement, a familiar concept for nurse educators, and a topic ubiquitous in healthcare. This paper is suited for any user curious about designing open educational resources with consideration of key elements to produce quality and educational resources that support excellence in nursing pedagogy. We begin by providing a background to our specific project followed by a discussion of the planning phase, the design phase, and other considerations. The e-textbook falls under a Creative Commons license and can be accessed for free by educators and learners. Résumé Le développement récent de ressources éducatives en libre accès a permis une accessibilité beaucoup plus grande, comme jamais auparavant, au matériel avec licences ouvertes dans le domaine public. Les ressources éducatives en libre accès sont des outils d’apprentissage, comme les manuels scolaires, qui sont offerts gratuitement et qui sont généralement accessibles en ligne. En dépit de la prolifération des ressources éducatives libres, bon nombre de professeurs sont toujours peu familiers avec la nature et le processus de production de telles ressources. L’objectif de ce texte de discussion est de partager notre expérience du développement d’un e-manuel de formation en libre accès, pour les étudiants inscrits dans les programmes postsecondaires pour la profession infirmière et les autres professions de la santé, tout en soulignant des conseils pratiques pour les professeurs. L’exemple, cité en référence dans ce texte, est axé sur la prise des signes vitaux, un concept familier aux infirmières enseignantes et un sujet omniprésent dans le domaine de la santé. Cet article convient à toute personne curieuse d’en apprendre davantage sur la conception des ressources éducatives en libre accès en tenant compte d’éléments clés afin de produire des ressources éducatives de qualité qui appuient l’excellence de la pédagogie en soins infirmiers. Nous établissons d’abord le contexte du projet particulier suivi d’une discussion sur la phase de planification, la phase de conception et sur d’autres éléments à considérer. Le e-manuel relève d’une licence Creative Commons et est accessible gratuitement pour les professeurs et les étudiants

Differential expression of microRNA-206 and its target genes in pre-eclampsia

Objectives: Pre-eclampsia is a multi-system disease that significantly contributes to maternal and fetal morbidity and mortality. In this study, we used a non-biased microarray approach to identify novel circulating miRNAs in maternal plasma that may be associated with pre-eclampsia. Methods: Plasma samples were obtained at 16 and 28 weeks of gestation from 18 women who later developed pre-eclampsia (cases) and 18 matched women with normotensive pregnancies (controls). We studied miRNA expression profiles in plasma and subsequently confirmed miRNA and target gene expression in placenta samples. Placental samples were obtained from an independent cohort of 19 women with pre-eclampsia matched with 19 women with normotensive pregnancies. Results: From the microarray, we identified 1 miRNA that was significantly differentially expressed between cases and controls at 16 weeks of gestation and 6 miRNAs that were significantly differentially expressed at 28 weeks. Following qPCR validation only one, miR-206, was found to be significantly increased in 28 week samples in women who later developed pre-eclampsia (1.4 fold change ± 0.2). The trend for increase in miR-206 expression was mirrored within placental tissue from women with pre-eclampsia. In parallel, IGF-1, a target gene of miR-206, was also found to be down-regulated (0.41 ± 0.04) in placental tissue from women with pre-eclampsia. miR-206 expression was also detectable in myometrium tissue and trophoblast cell lines. Conclusions: Our pilot study has identified miRNA-206 as a novel factor up-regulated in pre-eclampsia within the maternal circulation and in placental tissue

Integrating donor conception into identity development: adolescents in fatherless families.

OBJECTIVE: To study the processes by which donor-conceived children incorporate donor conception into their subjective sense of identity. DESIGN: Cross-sectional. SETTING: Family homes. PATIENT(S): Nineteen donor-conceived adolescents. INTERVENTION(S): Administration of an interview and questionnaire. MAIN OUTCOME MEASURE(S): The mother-child relationship was assessed through the Friends and Family Interview, a semistructured interview designed to assess adolescents' security of attachment in terms of secure-autonomous, insecure-dismissive, insecure-preoccupied, and insecure-disorganized attachment patterns. The Donor Conception Identity Questionnaire assessed adolescents' thoughts and feelings about donor conception, yielding two factors: [1] curiosity about donor conception and [2] avoidance of donor conception. RESULT(S): Statistically significant associations were found between the Curiosity scale and the secure-autonomous and insecure-dismissing attachment ratings. Adolescents with secure-autonomous attachment patterns were more interested in exploring donor conception whereas those with insecure-dismissing patterns were less likely to express curiosity. Insecure-disorganized attachment ratings were statistically significantly correlated with the Avoidance scale, indicating higher levels of negative feelings about donor conception. CONCLUSION(S): The results of this study of the influence of parent-child relationships on thoughts and feelings about donor conception in adolescence suggest that the valence of the parent-child relationship influences adolescents' appraisal of their donor conception within the context of their growing sense of identity.This research was supported by a Wellcome Trust Senior Investigator Award [097857/Z/11/Z].This is the final version of the article. It first appeared from Elsevier via https://doi.org/ 10.1016/j.fertnstert.2016.02.03

Col11a2 Deletion Reveals the Molecular Basis for Tectorial Membrane Mechanical Anisotropy

The tectorial membrane (TM) has a significantly larger stiffness in the radial direction than other directions, a prominent mechanical anisotropy that is believed to be critical for the proper functioning of the cochlea. To determine the molecular basis of this anisotropy, we measured material properties of TMs from mice with a targeted deletion of Col11a2, which encodes for collagen XI. In light micrographs, the density of TM radial collagen fibers was lower in Col11a2 –/– mice than wild-types. Tone-evoked distortion product otoacoustic emission and auditory brainstem response measurements in Col11a2 –/– mice were reduced by 30–50 dB independent of frequency as compared with wild-types, showing that the sensitivity loss is cochlear in origin. Stress-strain measurements made using osmotic pressure revealed no significant dependence of TM bulk compressibility on the presence of collagen XI. Charge measurements made by placing the TM as an electrical conduit between two baths revealed no change in the density of charge affixed to the TM matrix in Col11a2 –/– mice. Measurements of mechanical shear impedance revealed a 5.5 ± 0.8 dB decrease in radial shear impedance and a 3.3 ± 0.3 dB decrease in longitudinal shear impedance resulting from the Col11a2 deletion. The ratio of radial to longitudinal shear impedance fell from 1.8 ± 0.7 for TMs from wild-type mice to 1.0 ± 0.1 for those from Col11a2 –/– mice. These results show that the organization of collagen into radial fibrils is responsible for the mechanical anisotropy of the TM. This anisotropy can be attributed to increased mechanical coupling provided by the collagen fibrils. Mechanisms by which changes in TM material properties may contribute to the threshold elevation in Col11a2 –/– mice are discussed.National Institutes of Health (U.S.) (Grant R01-DC00238

C-reactive protein and the risk of developing type 2 diabetes in Aboriginal Australians

OBJECTIVE: To investigate the association between C-reactive protein (CRP) and the risk of developing diabetes in Aboriginal Australians. RESEARCH DESIGN AND METHODS: High sensitivity CRP levels were measured in 620 Aboriginal participants aged 20-74 years free from diabetes at baseline in a remote community in the Northern Territory of Australia. Participants were followed for a median of 11 years to identify newly diagnosed cases of diabetes. Cox proportional hazards models were used to assess the relationship of CRP levels with the risk of developing diabetes over the follow-up period. RESULTS: A total of 109 participants were newly diagnosed with diabetes. Incident rates were 10.8, 16.6 and 28.8 per 1000 person-years for people in the lower, middle and upper tertile groups of baseline CRP levels, respectively. After adjusting for age, sex, BMI, baseline glucose regulation status, total cholesterol, urine albumin to creatinine ratio, systolic blood pressure, smoking and alcohol drinking, the association between diabetes and CRP remained significant, with a hazard ratio of 1.23 (95% confidence interval (CI) 1.05, 1.45) corresponding to a doubling in CRP values. Similarly, the adjusted hazard ratio for development of diabetes in people in the upper tertile versus the bottom two tertiles of CRP was 1.75 (95% CI 1.19, 2.56). CONCLUSIONS: CRP is independently associated with the development of diabetes in Aboriginal people. Our findings support a role of inflammation in the etiology of diabetes in the high risk population of Aboriginal Australians

Integrins Form an Expanding Diffusional Barrier that Coordinates Phagocytosis

Phagocytosis is initiated by lateral clustering of receptors, which in turn activates Src-family kinases (SFKs). Activation of SFKs requires depletion of tyrosine phosphatases from the area of particle engagement. We investigated how the major phosphatase CD45 is excluded from contact sites, using single-molecule tracking. The mobility of CD45 increased markedly upon engagement of Fcγ receptors. While individual CD45 molecules moved randomly, they were displaced from the advancing phagocytic cup by an expanding diffusional barrier. By micropatterning IgG, the ligand of Fcγ receptors, we found that the barrier extended well beyond the perimeter of the receptor-ligand engagement zone. Second messengers generated by Fcγ receptors activated integrins, which formed an actin-tethered diffusion barrier that excluded CD45. The expanding integrin wave facilitates the “zippering” of Fcγ receptors onto the target and integrates the information from sparse receptor-ligand complexes, coordinating the progression and ultimate closure of the phagocytic cup