DGGE profiles for the 16S rRNA amplicons of females originating from the <i>Wol-</i> and <i>Wol+</i> matriline and females of the Damvallei (D) and Walenbos (W) population.

<p>Clones indicate the use of DNA amplicons resulting from the cloning study. Bands B and D: <i>Rickettsia</i> endosymbiont; C and L: <i>Wolbachia</i> endosymbiont; J: <i>Acinetobacter</i> sp.; sequences from other bands were ambiguous.</p

Taxonomic affiliation of the sequenced bands obtained by DGGE of Walenbos and Damvallei females.

<p>Taxonomic affiliation of the sequenced bands obtained by DGGE of Walenbos and Damvallei females.</p

Proportion of paired 100bp Illumina reads from the 16S rRNA-V4 and 16S rRNA-V3 region assigned to the different bacterial taxa in the dwarf spider <i>Oedothorax gibbosus</i>.

<p><i>Wol+</i> and <i>Wol</i>- are individual females from maternal lines infected with and without <i>Wolbachia</i> respectively. DAM and WAL represent samples consisting of ten pooled wild caught females from population Damvallei and Walenbos respectively. Numbers above bars represent the number of reads in millions.</p

Phylogenetic position of the 16S rRNA gene sequence of <i>Rhabdochlamydia</i> of <i>Oedothorax gibbosus</i>.

<p>A p-distance based Neighbour Joining tree was constructed as implemented in MEGA 6 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117297#pone.0117297.ref047" target="_blank">47</a>] on a subset of <i>Chlamydiae</i> sequences available at Genbank. Percentage bootstrap support was generated for the nodes. Accession numbers are given between brackets, <i>Rhabdochlamydia</i> found in <i>Oedothorax gibbosus</i> is marked with an asterisk.</p

Phenological data

Raw data presented in Fig. 4. ('nfem' = total number of females (mated and unmated); 'n unmated fem' = number of unmated females; n tub = number of tuberosus males; n gib = number of gibbosus males; n juv = number of immature individuals

Data mating experiment

Raw data of the mating experiment. First sheet present the data of a single male exposure (corresponding to left panel of Fig. 3), second sheet multiple male exposure (corresponding to right panel Fig. 3). Column 1 and 2 describe the morph type of the respectively first and second male to which a female was mated (t = tuberosus, g = gibbosus). "ncop" = number of copulations in a session; "cop" = binary variable describing if a copulation took place (1 = yes; 0 = no)

Sequence_alignments

Sequence alignments of Pogonus chalcesu mitochondrial NADP+-dependent isocitrate dehydrogenase (mtIdh), cytoplasmic NADP+-dependent isocitrate dehydrogenase (cytIdh), enolase, cytochrome oxidase subunit one (cox1), a mitochondrial region spanning the NADH subunit I (180 bp), the tRNA-Leu gene (64 bp) and the 16S rRNA gene (111 bp) and cytochrome b (cob; 468 bp

Location and codes

Table containing code, locations, species names and GenBank accession number

Calo_CAD_haplos.info

List of individuals in which each cad haplotype was found

Data_and_SAS_code

1. Raw data for escape behavior of field captured adults to inundation. 2. Raw data for adult escape behavior to inundation following tidal/seasonal treatment of larvae and pupa