Physically and Sexually Violent Juvenile Offenders: A Comparative Study of Victimization History Variables

The primary purpose of this study was to examine and compare physically and sexually violent juvenile offenders (PVJOs and SVJOs) to determine whether specific factors in their abuse histories, if present, tend to be associated with-the type of violent offense pattern they exhibit. The Youth Experiences and Behaviors Structured Interview (YEBSI)--an instrument which assesses for primary (victimization), secondary (witnessing), and perpetrated abuse of an emotional, physical, and sexual nature, by and/or toward family members, acquaintances, strangers, and animals--was developed by the primary researcher for use in this study. Thirty-six PVJOs and 30 SVJOs were interviewed. Results indicated that the YEBSI demonstrated high levels of internal consistency reliability and a very high level of interrater reliability. Various descriptive statistical, scale, and subscale correlations for the YEBSI were provided. Very high percentages of both groups reported experiencing and witnessing all types of abuse. In all cases, a similar or larger percentage of SVJOs reported histories of primary and secondary abuse. SVJOs reported more severe levels of emotional abuse, similar severity levels of physical abuse, and less extremely severe levels of sexual abuse than did PVJOs. Family members and acquaintances (as compared to strangers) tended to be far more frequently reported as perpetrators by respondents. Composite primary and secondary abuse scores were moderately correlated with abuse perpetration scores for SVJOs and strongly correlated with abuse perpetration scores for PVJOs. For emotional, family, acquaintance, and stranger abuse, reported primary-secondary abuse scores were found to be most highly correlated with abuse perpetration scores of the same nature (e.g., emotional abuse history-witness scores best correlated with physical abuse perpetration scores and family abuse history-witness scores best correlated with perpetration scores against family members) Finally, the classification variables correctly predicted 75% of those in the physically violent group and 67% of those in the sexually violent group, with an overall hit rate of 71%. Examination of the discriminant function-variable correlations in this study indicates that it was primarily the emotional, family-perpetrated, and sexual abuse subscales that defined the function. Theoretical interpretations and implications for these results are provided

Is There a Connection? An Exploratory Study of Abuse Experiences and Perpetration Patterns Among College Males

The purpose of this study was to examine self-reported experiences of primary, secondary, and perpetrated abuse of an emotional, physical, and sexual nature among a male, nonclinical, noncriminal sample. One hundred forty-two subjects completed the Youth Experiences and Behaviors Questionnaire (YEBQ) (developed by the author) and the Conflict Tactics Scale (CTS). Results indicated that overall, the YEBQ demonstrated moderate to high levels of internal consistency reliability and moderate levels of concurrent and divergent validity. Various descriptive statistics, scale, and subscale correlations for the YEBQ were provided. Results indicated that secondary abuse information was most predictive of later abuse perpetration with the exception of sexual abuse. Primary and secondary abuse information was found to be most predictive of abuse perpetration types of the same nature (e.g., emotional abuse history/witness scores best predicted emotional abuse perpetration scores and family abuse history/witness scores best predicted abuse perpetration toward family members scores) except in the cases of sexual abuse and stranger abuse. Theoretical interpretations and implications for these results are provided

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Identification of Post-cardiac Arrest Blood Pressure Thresholds Associated With Outcomes in Children: An ICU-Resuscitation Study

INTRODUCTION: Though early hypotension after pediatric in-hospital cardiac arrest (IHCA) is associated with inferior outcomes, ideal post-arrest blood pressure (BP) targets have not been established. We aimed to leverage prospectively collected BP data to explore the association of post-arrest BP thresholds with outcomes. We hypothesized that post-arrest systolic and diastolic BP thresholds would be higher than the currently recommended post-cardiopulmonary resuscitation BP targets and would be associated with higher rates of survival to hospital discharge. METHODS: We performed a secondary analysis of prospectively collected BP data from the first 24 h following return of circulation from index IHCA events enrolled in the ICU-RESUScitation trial (NCT02837497). The lowest documented systolic BP (SBP) and diastolic BP (DBP) were percentile-adjusted for age, height and sex. Receiver operator characteristic curves and cubic spline analyses controlling for illness category and presence of pre-arrest hypotension were generated exploring the association of lowest post-arrest SBP and DBP with survival to hospital discharge and survival to hospital discharge with favorable neurologic outcome (Pediatric Cerebral Performance Category of 1-3 or no change from baseline). Optimal cutoffs for post-arrest BP thresholds were based on analysis of receiver operator characteristic curves and spline curves. Logistic regression models accounting for illness category and pre-arrest hypotension examined the associations of these thresholds with outcomes. RESULTS: Among 693 index events with 0-6 h post-arrest BP data, identified thresholds were: SBP \u3e 10th percentile and DBP \u3e 50th percentile for age, sex and height. Fifty-one percent (n = 352) of subjects had lowest SBP above threshold and 50% (n = 346) had lowest DBP above threshold. SBP and DBP above thresholds were each associated with survival to hospital discharge (SBP: aRR 1.21 [95% CI 1.10, 1.33]; DBP: aRR 1.23 [1.12, 1.34]) and survival to hospital discharge with favorable neurologic outcome (SBP: aRR 1.22 [1.10, 1.35]; DBP: aRR 1.27 [1.15, 1.40]) (all p \u3c 0.001). CONCLUSIONS: Following pediatric IHCA, subjects had higher rates of survival to hospital discharge and survival to hospital discharge with favorable neurologic outcome when BP targets above a threshold of SBP \u3e 10th percentile for age and DBP \u3e 50th percentile for age during the first 6 h post-arrest

Rothmund-Thomson syndrome-like RECQL4 truncating mutations cause a haploinsufficient low-bone-mass phenotype in mice

Rothmund-Thomson syndrome (RTS) is an autosomal recessive disorder characterized by defects in the skeletal system, such as bone hypoplasia, short stature, low bone mass, and an increased incidence of osteosarcoma. RTS type 2 patients have germ line compound biallelic protein-truncating mutations of RECQL4. As existing murine models employ Recql4 null alleles, we have attempted to more accurately model RTS by generating mice with patient-mimicking truncating Recql4 mutations. Truncating mutations impaired the stability and subcellular localization of RECQL4 and resulted in homozygous embryonic lethality and a haploinsufficient low-bone mass phenotype. Combination of a truncating mutation with a conditional Recql4 null allele demonstrated that the skeletal defects were intrinsic to the osteoblast lineage. However, the truncating mutations did not promote tumorigenesis. We utilized murine Recql4 null cells to assess the impact of human RECQL4 mutations using an in vitro complementation assay. While some mutations created unstable protein products, others altered subcellular localization of the protein. Interestingly, the severity of the phenotypes correlated with the extent of protein truncation. Collectively, our results reveal that truncating RECQL4 mutations in mice lead to an osteoporosis-like phenotype through defects in early osteoblast progenitors and identify RECQL4 gene dosage as a novel regulator of bone mass

Host factors are associated with vaginal microbiome structure in pregnancy in the ECHO Cohort Consortium

Using pooled vaginal microbiota data from pregnancy cohorts (N = 683 participants) in the Environmental influences on Child Health Outcomes (ECHO) Program, we analyzed 16S rRNA gene amplicon sequences to identify clinical and demographic host factors that associate with vaginal microbiota structure in pregnancy both within and across diverse cohorts. Using PERMANOVA models, we assessed factors associated with vaginal community structure in pregnancy, examined whether host factors were conserved across populations, and tested the independent and combined effects of host factors on vaginal community state types (CSTs) using multinomial logistic regression models. Demographic and social factors explained a larger amount of variation in the vaginal microbiome in pregnancy than clinical factors. After adjustment, lower education, rather than self-identified race, remained a robust predictor of L. iners dominant (CST III) and diverse (CST IV) (OR = 8.44, 95% CI = 4.06–17.6 and OR = 4.18, 95% CI = 1.88–9.26, respectively). In random forest models, we identified specific taxonomic features of host factors, particularly urogenital pathogens associated with pregnancy complications (Aerococcus christensenii and Gardnerella spp.) among other facultative anaerobes and key markers of community instability (L. iners). Sociodemographic factors were robustly associated with vaginal microbiota structure in pregnancy and should be considered as sources of variation in human microbiome studies