CO2 treatment increases glucosinolate hydrolysis products in two Arabidopsis thaliana accessions

Brassicales include many vegetables of nutritional interest because the hydrolysis products of their phytochemicals, the glucosinolates, have health-promoting properties. So far, the impact of rising CO2 concentrations on glucosinolates and their hydrolysis is unclear. Applying a modified atmosphere, we exposed two Arabidopsis thaliana accessions that differ in their glucosinolate hydrolysis behavior, namely Hi-0 and Bur-0, to elevated CO2 concentrations. Glucosinolates and their hydrolysis products were analyzed using UHPLC-DAD-MS and GC-MS. CO2 treatment increased indicators of primary production, such as biomass, leaf area and electron transport rate, and increased gluco-sinolate levels in Bur-0, but not Hi-0. Significantly, released glucosinolate hydrolysis product levels increased by up to 122% in Bur-0 due to increased epithionitrile formation. Likewise, in Hi-0 glucosinolate hydrolysis product levels increased after CO2 treatment by up to 67%, caused by enhanced nitrile and to some extent isothiocyanate formation. In addition, more alkenyl rather than alkyl glucosinolates were formed in Bur-0 under elevated CO2, thus changing the glucosinolate profile compositions. As CO2 treatment enhanced primary production but also overall glucosinolate hydrolysis pro-duct formation, it is conceivable to recycle excess CO2 by using it as supplement greenhouse gas to produce high-quality food

Biologische Bodenentseuchung für eine umweltgerechte und intensive Gehölzproduktion

Der wiederholte Nachbau von Gehölzen der Familie der Rosaceae führt zu einer Nachbaukrankheit, die als Bodenmüdigkeit beschrieben wird. Eine wirksame Bekämpfung der Bodenmüdigkeit war die chemische Desinfektion mit Basamid® Granulat, basierend auf der Freisetzung eines Isothiocyanates (ITC). Die Zulassung von Basamid® Granulat ist in Deutschland abgelaufen. Eine Alternative könnte die Biofumigation darstellen. Bei der Biofumigation werden Glucosinolat(GS)-haltige Pflanzenteile von Vertretern der Brassicaceae in den Boden eingearbeitet. Die toxischen GS-Abbauprodukte, vor allem ITC, führen zu einer biologischen Bodendesinfektion. In dem berichteten Projekt wurde die Wirkung der ein- und zweijährigen Biofumigation von Brassica juncea und Raphanus sativus mit dem Anbau von Tagetes patula NEMAMIX, der Anwendung von Basamid® Granulat und des fortgesetzten Nachbaus der Indikatorpflanzen Malus sylvestris ‘Bittenfelder, Malus M4 bzw. M106 und Rosa corymbifera ‘Laxa’ verglichen. Das beste Wachstum der Indikatorpflanzen erbrachte ein vorausgegangener, zweijähriger Anbau von Tagetes patula NEMAMIX. Die praxisübliche Anwendung von Basamid® Granulat zeigte wenig Wirkung. Vermutlich war der Boden zum Anwendungstermin zu trocken. Eine geringe Wirkung erbrachte auch die ein- oder auch zweijährige Biofumigation mit Brassica juncea und Raphanus sativus. Auch die im Rahmen der Projektverlängerung zusätzlich geprüfte Einarbeitung von Brassica juncea-Samenmehl, die Verwendung der speziellen Saatgutmischung viterra®BIOFUMIGATION zur klassischen Biofumigation sowie die Dämpfung des Bodens mit Hilfe des CombiMIXERs an zwei nachbaukranken Baumschulstandorten brachten nicht die erhofften positive Effekte auf das Pflanzenwachstum der Indikatorpflanzen. Wieder war die einjährige Kultur von Tagetes die erfolgreichste Var. im Vergleich zum unbehandelten nachbaukranken Boden. Es konnten nur wenig positive Effekte auf das Wachstum der Indikatorpflanzen statistisch gesichert werden. Selbst in den Fällen, in denen signifikante Unterschiede auftraten, reichten diese Effekte aus Sicht der Praxis bei weitem nicht aus, um die durch Minderwuchs entstehenden Ertragsverluste auszugleichen. Mögliche Gründe für die schlechte Wirkung der geprüften Varianten werden diskutiert

Advanced Research on Glucosinolates in Food Products

Glucosinolate-containing foods, such as vegetables from the plant order Brassicales and its derivative products, are valued for their health-beneficial properties [...

Isothiocyanates, Nitriles, and Epithionitriles from Glucosinolates Are Affected by Genotype and Developmental Stage in Brassica oleracea Varieties

Vegetables of the Brassica oleracea group, such as broccoli, cauliflower, and cabbage, play an important role for glucosinolate consumption in the human diet. Upon maceration of the vegetable tissue, glucosinolates are degraded enzymatically to form volatile isothiocyanates, nitriles, and epithionitriles. However, only the uptake of isothiocyanates is linked to the cancer-preventive effects. Thus, it is of great interest to evaluate especially the isothiocyanate formation. Here, we studied the formation of glucosinolates and their respective hydrolysis products in sprouts and fully developed vegetable heads of different genotypes of the five B. oleracea varieties: broccoli, cauliflower as well as white, red, and savoy cabbages. Further, the effect of ontogeny (developmental stages) during the head development on the formation of glucosinolates and their respective hydrolysis products was evaluated at three different developmental stages (mini, fully developed, and over-mature head). Broccoli and red cabbage were mainly rich in 4-(methylsulfinyl)butyl glucosinolate (glucoraphanin), whereas cauliflower, savoy cabbage and white cabbage contained mainly 2-propenyl (sinigrin) and 3-(methylsulfinyl)propyl glucosinolate (glucoiberin). Upon hydrolysis, epithionitriles or nitriles were often observed to be the main hydrolysis products, with 1-cyano-2,3-epithiopropane being most abundant with up to 5.7 μmol/g fresh weight in white cabbage sprouts. Notably, sprouts often contained more than 10 times more glucosinolates or their hydrolysis products compared to fully developed vegetables. Moreover, during head development, both glucosinolate concentrations as well as hydrolysis product concentrations changed and mini heads contained the highest isothiocyanate concentrations. Thus, from a cancer-preventive point of view, consumption of mini heads of the B. oleracea varieties is recommended

Biofumigation for Fighting Replant Disease- A Review

Replant disease is a soil (micro-) biome-based, harmfully-disturbed physiological and morphological reaction of plants to replanting similar cultures on the same sites by demonstrating growth retardation and leading to economic losses especially in Rosaceae plant production. Commonly, replant disease is overcome by soil fumigation with toxic chemicals. With chemical soil fumigation being restricted in many countries, other strategies are needed. Biofumigation, which is characterized by the incorporation of Brassicaceae plant materials into soil, is a promising method. We review the potential of biofumigation in the fight against replant disease. Biofumigation using optimized Brassicaceae seed meal compositions in combination with replant disease tolerant plant genotypes shows promising results, but the efficacy is still soil and site-dependent. Therefore, future studies should address the optimal timing as well as amount and type of incorporated plant material and environmental conditions during incubation in dependence of the soil physical and chemical characteristics

Seasonal Variation of Glucosinolate Hydrolysis Products in Commercial White and Red Cabbages (Brassica oleracea var. capitata)

Brassica vegetables contain glucosinolates, which are well-known for their potential to form health-promoting isothiocyanates. Among those crucifers, white and red cabbage are commonly consumed vegetables, exhibiting different glucosinolate and hydrolysis profiles thereof. Regarding the health beneficial effects from these vegetables, more information, especially concerning the seasonal variation of glucosinolate profiles and the formation of their bioactive hydrolysis products in commercial cabbages, is needed. In this study, glucosinolates and glucosinolate hydrolysis product profiles in red and white cabbages from three different food retailers were monitored over six different sampling dates across the selling season in autumn. For the first time, it was shown that, while glucosinolate profiles were similar in each cabbage variety, glucosinolate hydrolysis product profiles and hydrolysis behavior varied considerably over the season. The highest total isothiocyanate concentrations were observed in conventional red (1.66 μmol/g FW) and organic white (0.93 μmol/g FW) cabbages purchased at the first sampling date in September. Here, red cabbage was with up to 1.06 μmol/g FW of 4-(methylsulfinyl)butyl isothiocyanate (sulforaphane), an excellent source for this health-promoting isothiocyanate. Cabbages purchased 11 weeks later in autumn released lower levels of isothiocyanates, but mainly nitriles and epithionitriles. The results indicate that commercial cabbages purchased in early autumn could be healthier options than those purchased later in the year

Seasonal Variation of Glucosinolate Hydrolysis Products in Commercial White and Red Cabbages (Brassica oleracea var. capitata)

Brassica vegetables contain glucosinolates, which are well-known for their potential to form health-promoting isothiocyanates. Among those crucifers, white and red cabbage are commonly consumed vegetables, exhibiting different glucosinolate and hydrolysis profiles thereof. Regarding the health beneficial effects from these vegetables, more information, especially concerning the seasonal variation of glucosinolate profiles and the formation of their bioactive hydrolysis products in commercial cabbages, is needed. In this study, glucosinolates and glucosinolate hydrolysis product profiles in red and white cabbages from three different food retailers were monitored over six different sampling dates across the selling season in autumn. For the first time, it was shown that, while glucosinolate profiles were similar in each cabbage variety, glucosinolate hydrolysis product profiles and hydrolysis behavior varied considerably over the season. The highest total isothiocyanate concentrations were observed in conventional red (1.66 μmol/g FW) and organic white (0.93 μmol/g FW) cabbages purchased at the first sampling date in September. Here, red cabbage was with up to 1.06 μmol/g FW of 4-(methylsulfinyl)butyl isothiocyanate (sulforaphane), an excellent source for this health-promoting isothiocyanate. Cabbages purchased 11 weeks later in autumn released lower levels of isothiocyanates, but mainly nitriles and epithionitriles. The results indicate that commercial cabbages purchased in early autumn could be healthier options than those purchased later in the year