8 research outputs found
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Determining enantiomeric excess using indicator-displacement assays
The invention includes an enantioselective indicator-displacement assay useful to determine enantiomeric excess (ee) enantiomeric samples calorimetrically. Determination may be by inspection of color with the naked eye, spectrographic measurement, or mathematical calculation. Concentration may also be determined. The assay may involve two independent absorption measurements. On suitable group of enantiomeric molecules to be assayed include α-hydroxyacids. The inherent relationship between the absorbance of the indicator-displacement ensemble and the overall concentration and ee of the analyte is established through solution equilibria. The invention also includes use of the assay in drug screening and manufacturing, high throughput screening of catalysts and kits for use in conducting assays of the invention.Board of Regents, University of Texas Syste
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Metallosupramolecular chemistry in aqueous solutions: applications in ribonuclease mimicry and molecular sensing
textThe use of kinetically labile metal ions to template designed interactions between
organic ligands in aqueous solutions is described. This strategy is employed to target
small biological molecules using coordinatively unsaturated metalloreceptors, which
perform specific functions as a result of their association modes. Within this framework,
two distinct examples are presented. The first case involves a tridentate Zn(II) complex
with guanidinium-containing auxiliary groups that are constrained within the proximity
of the metal center. This defines a bifunctional tetracationic cleft, which promotes the
hydrolytic cleavage of a dinucleotide with cooperativity between the guanidinium groups
and the Zn(II) center. The synergistic use of guanidinium residues and a metal center is
also central to the activity of staphylococcal nuclease, which is one of the most active
known nuclease enzymes. The second example concerns the incorporation asymmetric
metal complexes into multi-component, colorimetric enantiosensors for α-amino acids.
After several unsuccessful attempts, chiral vicinal diamine-Cu(II) complexes were
identified as suitable receptors, and accurate spectrophotometric enantiomeric excess
determinations were made for several hydrophobic α-amino acids. This system was
extended for the construction of an enantioselective differential array sensor, which
segregates chemo- and enantiomeric variance when sensors of opposite enantiomeric
preference are included. The modality of this analysis likely parallels that of the
mammalian gustatory response to α-amino acids, as actual amino acid taste receptor
proteins respond with opposing enantioselectivities.Chemistry and BiochemistryChemistr
Recommended from our members
Determining enantiomeric excess using indicator-displacement assays
The invention includes an enantioselective indicator-displacement assay useful to determine enantiomeric excess (ee) enantiomeric samples colorimetrically. Determination may be by inspection of color with the naked eye, spectrographic measurement, or mathematical calculation. Concentration may also be determined. The assay may involve two independent absorption measurements. On suitable group of enantiomeric molecules to be assayed include α-hydroxy acids. The inherent relationship between the absorbance of the indicator-displacement ensemble and the overall concentration and ee of the analyte is established through solution equilibria. The invention also includes use of the assay in drug screening and manufacturing, high throughput screening of catalysts and kits for use in conducting assays of the invention.Board of Regents, University of Texas Syste