New building family house

Bakalářská práce řeší návrh rodinného domu ve Vranově. Objekt je navržen jako dvoupodlažní budova, umístěna na absolutně srovnaném terénu, který je otevřen do zahrady směrem na jih. Vstup do domu je řešen v prostřední části, orientované na severní stranu. V 1NP se nachází komunikační prostory, ze kterých je přístup jak do garáže a technického zázemí, tak do kuchyně, jídelny i obývacího pokoje. Rovněž na tento prostor navazuje schodiště do 2NP. Zde se nachází klidová zóna – ložnice, dětské pokoje, pokoj pro hosty a dvě koupelny. Svislé konstrukce jsou navrženy z dutinového keramického zdiva bez dalšího zateplení. Stropní konstrukce jsou skládané. Objekt je zastřešen plochou střechou se sklonem 3%.This bachelor s thesis deals with the creation of a plan for a detached house in Vranov. The building is designed as a two storey building, situated in absolutely leveled terrain, which opens to the south. The entrance is located in the central part of the house, oriented to the nord. In the first floor, there are communication facilities which also provide access to the garrage and technical basement, as well as the kitchen, dining room and the living room. This space is also the way to enter the second floor, which contains the private zone, in adition to the bedroom, children s room, guest room and two bathrooms. Vertical constructions are designed of hollow bricks without the need of any thermal insulation. Floor structures are rib- and-filler refabricated. The building is covered with a 3% slope flat roof.

Conservation of Silk Genes in Trichoptera and Lepidoptera

Larvae of the sister orders Trichoptera and Lepidoptera are characterized by silk secretion from a pair of labial glands. In both orders the silk filament consists of heavy (H)- and light (L)-chain fibroins and in Lepidoptera it also includes a P25 glycoprotein. The L-fibroin and H-fibroin genes of Rhyacophila obliterata and Hydropsyche angustipennis caddisflies have exon/intron structuring (seven exons in L-fibroin and two in H-fibroin) similar to that in their counterparts in Lepidoptera. Fibroin cDNAs are also known in Limnephilus decipiens, representing the third caddisfly suborder. Amino acid sequences of deduced L-fibroin proteins and of the terminal H-fibroin regions are about 50% identical among the three caddisfly species but their similarity to lepidopteran fibroins is <25%. Positions of some residues are conserved, including cysteines that were shown to link the L-fibroin and H-fibroin by a disulfide bridge in Lepidoptera. The long internal part of H-fibroins is composed of short motifs arranged in species-specific repeats. They are extremely uniform in R. obliterata. Motifs (SX)n, GGX, and GPGXX occur in both Trichoptera and Lepidoptera. The trichopteran H-fibroins further contain charged amphiphilic motifs but lack the strings of alanines or alanine-glycine dipeptides that are typical lepidopteran motifs. On the other hand, sequences composed of a motif similar to ERIVAPTVITR surrounded by the (SX)4-6 strings and modifications of the GRRGWGRRG motif occur in Trichoptera and not in Lepidoptera

Reconstruction of the multifunctional room music school with an emphasis on acoustics

The aim of the diploma thesis was to design the reconstruction of the music hall in the existing building of the private music school D-Music. The building is located in Kromeriz, cadastral area Kromeriz. I emphasize the design from the perspective of acoustics. The thesis also evaluated the noise load from the adjacent road II. class, for design hole fillings. It is a five-storey building with one underground and four above-ground floors. The music hall is located on the first floor and is designed multifunctionaly - for music with mobile acoustic absorbers for speech

Reconstruction of the multifunctional room music school with an emphasis on acoustics

Předmětem diplomové práce bylo vypracovat návrh projektové dokumentace pro rekonstrukci hudebního sálu ve stávajícím objektu soukromé hudební školy D-Music. Objekt se nachází ve městě Kroměříž, katastrální území Kroměříž. Ve své práci kladu v návrhu důraz na prostorovou a stavební akustiku. V rámci diplomové práce byla vyhodnocena i hluková zátěž z přilehlé komunikace II. třídy, pro návrh vhodných výplní otvorů. Jedná se o pětipodlažní objekt s jedním podzemním a čtyřmi nadzemními podlažími. Hudební sál je umístěn v 1 NP. Sál je navržen multifunkčně - pro přednes hudby, s mobilními akustickými úpravami pro mluvené slovo.The aim of the diploma thesis was to design the reconstruction of the music hall in the existing building of the private music school D-Music. The building is located in Kromeriz, cadastral area Kromeriz. I emphasize the design from the perspective of acoustics. The thesis also evaluated the noise load from the adjacent road II. class, for design hole fillings. It is a five-storey building with one underground and four above-ground floors. The music hall is located on the first floor and is designed multifunctionaly - for music with mobile acoustic absorbers for speech.

Structural diversity of prothoracicotropins and bombyxins in Lepidoptera

Structural diversity of prothoracicotropins and bombyxins in Lepidoptera

Use of Carabids for the Post-Market Environmental Monitoring of Genetically Modified Crops

Post-market environmental monitoring (PMEM) of genetically modified (GM) crops is required by EU legislation and has been a subject of debate for many years; however, no consensus on the methodology to be used has been reached. We explored the suitability of carabid beetles as surrogates for the detection of unintended effects of GM crops in general PMEM surveillance. Our study combines data on carabid communities from five maize field trials in Central Europe. Altogether, 86 species and 58,304 individuals were collected. Modeling based on the gradual elimination of the least abundant species, or of the fewest categories of functional traits, showed that a trait-based analysis of the most common species may be suitable for PMEM. Species represented by fewer than 230 individuals (all localities combined) should be excluded and species with an abundance higher than 600 should be preserved for statistical analyses. Sixteen species, representing 15 categories of functional traits fulfill these criteria, are typical dominant inhabitants of agroecocoenoses in Central Europe, are easy to determine, and their functional classification is well known. The effect of sampling year is negligible when at least four samples are collected during maize development beginning from 1 April. The recommended methodology fulfills PMEM requirements, including applicability to large-scale use. However, suggested thresholds of carabid comparability should be verified before definitive conclusions are drawn

Cry3Aa Toxin Is Not Suitable to Control Lepidopteran Pest Spodoptera littoralis (Boisd.)

The toxicity of the Bacillus thuringiensis (Bt) toxin Cry3Aa—originally used against the main potato pest, the Colorado potato beetle, Leptinotarsa decemlineata—was verified on this species and then evaluated against the Egyptian armyworm, Spodoptera littoralis, which is a pest of several economically important plants. Larvae of S. littoralis were fed a semi-artificial diet supplemented either with a recombinant or with a natural Bt toxin Cry3Aa and with the genetically engineered (GE) potato of variety Superior NewLeaf (SNL) expressing Cry3Aa. Cry3Aa concentration in the diet and the content in the leaves were verified via ELISA (enzyme-linked immunosorbent assay) and HPLC (high-performance liquid chromatography) during and at the end of the experiments. The biological effectiveness of the coleopteran-specific Cry3Aa with previous reports of activity against S. littoralis was tested on five different populations of S. littoralis larvae by monitoring 13 parameters involving development from penultimate instar, weight, the efficiency of food conversion to biomass, ability to reproduce, and mortality. Although some occasional differences occurred between the Cry3Aa treatments and control, any key deleterious effects on S. littoralis in this study were not confirmed. We concluded that the Cry3Aa toxin appears to be non-toxic to S. littoralis, and its practical application against this pest is unsuitable