Struggling readers? Using theory to complicate understandings of what it means to be literate in school

Theories guide many aspects of literacy research. In this article we describe four theoretical approaches that we have used in qualitative research with students who are perceived to struggle with reading in school, including: New Literacy Studies, Disability Studies in Education, Bioecological Systems Theory, and Cultural Historical Activity Theory. We provide a brief overview of each of the theories and then explain how we have used them to gain insights about students with whom we have worked in the context of our research. Although grounded in distinct perspectives, we argue that each of the theories are lenses through which we were better able to understand the complexities of students’ struggles with reading. We further argue that the theories are united in their ability to broaden the perspectives of researchers and teachers to better account for the social, cultural, and institutional factors that shape literacy teaching and learning in schools. We conclude by questioning the use of the term “struggling reader” and highlighting the implications of our individual theoretical frames and analyses for both research and practice.Accepted manuscrip

"Marginal pinching" in soap films

We discuss the behaviour of a thin soap film facing a frame element: the pressure in the Plateau border around the frame is lower than the film pressure, and the film thins out over a certain distance lambda(t), due to the formation of a well-localized pinched region of thickness h(t) and extension w(t). We construct a hydrodynamic theory for this thinning process, assuming a constant surface tension: Marangoni effects are probably important only at late stages, where instabilitites set in. We find lambda(t) ~ t^{1/4}, and for the pinch dimensions h(t) ~ t^{-1/2}$ and w(t) ~ t^{-1/4}. These results may play a useful role for the discussion of later instabilitites leading to a global film thinning and drainage, as first discussed by K. Mysels under the name ``marginal regeneration''.Comment: 7 pages, 2 figure

The PsbP domain protein 1 functions in the assembly of lumenal domains in photosystem I

Photosystem I (PS I) is a multisubunit membrane protein complex that functions as a light-driven plastocyanin-ferredoxin oxidoreductase. The PsbP domain protein 1 (PPD1; At4g15510) is located in the thylakoid lumen of plant chloroplasts and is essential for photoautotrophy, functioning as a PS I assembly factor. In this work, RNAi was used to suppress PPD1 expression, yielding mutants displaying a range of phenotypes with respect to PS I accumulation and function. These PPD1 RNAi mutants showed a loss of assembled PS I that was correlated with loss of the PPD1 protein. In the most severely affected PPD1 RNAi lines, the accumulated PS I complexes exhibited defects in electron transfer from plastocyanin to the oxidized reaction center P 700+. The defects in PS I assembly in the PPD1 RNAi mutants also had secondary effects with respect to the association of light-harvesting antenna complexes to PS I. Because of the imbalance in photosystem function in the PPD1 RNAi mutants, light-harvesting complex II associated with and acted as an antenna for the PS I complexes. These results provide new evidence for the role of PPD1 in PS I biogenesis, particularly as a factor essential for proper assembly of the lumenal portion of the complex. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc

N-terminal lipid modification is required for the stable accumulation of CyanoQ in Synechocystis sp. PCC 6803

© 2016 Juneau et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The CyanoQ protein has been demonstrated to be a component of cyanobacterial Photosystem II (PS II), but there exist a number of outstanding questions concerning its physical association with the complex. CyanoQ is a lipoprotein; upon cleavage of its transit peptide by Signal Peptidase II, which targets delivery of the mature protein to the thylakoid lumenal space, the N-terminal cysteinyl residue is lipid-modified. This modification appears to tether this otherwise soluble component to the thylakoid membrane. To probe the functional significance of the lipid anchor, mutants of the CyanoQ protein have been generated in Synechocystis sp. PCC 6803 to eliminate the N-terminal cysteinyl residue, preventing lipid modification. Substitution of the N-terminal cysteinyl residue with serine (Q-C22S) resulted in a decrease in the amount of detectable CyanoQ protein to 17% that of the wild-type protein. Moreover, the physical properties of the accumulated Q-C22S protein were consistent with altered processing of the CyanoQ precursor. The Q-C22S protein was shifted to a higher apparent molecular mass and partitioned in the hydrophobic phase in TX-114 phase-partitioning experiments. These results suggest that the hydrophobic N-terminal 22 amino acids were not properly cleaved by a signal peptidase. Substitution of the entire CyanoQ transit peptide with the transit peptide of the soluble lumenal protein PsbO yielded the Q-SS mutant and resulted in no detectable accumulation of the modified CyanoQ protein. Finally, the CyanoQ protein was present at normal amounts in the PS II mutant strains ΔpsbB and ΔpsbO, indicating that an association with PS II was not a prerequisite for stable CyanoQ accumulation. Together these results indicate that CyanoQ accumulation in Synechocystis sp. PCC 6803 depends on the presence of the N-terminal lipid anchor, but not on the association of CyanoQ with the PS II complex

The extrinsic proteins of photosystem II: update

© 2016, Springer-Verlag Berlin Heidelberg. Main conclusion: Recent investigations have provided important new insights into the structures and functions of the extrinsic proteins of Photosystem II. This review is an update of the last major review on the extrinsic proteins of Photosystem II (Bricker et al., Biochemistry 31:4623–4628 2012). In this report, we will examine advances in our understanding of the structure and function of these components. These proteins include PsbO, which is uniformly present in all oxygenic organisms, the PsbU, PsbV, CyanoQ, and CyanoP proteins, found in the cyanobacteria, and the PsbP, PsbQ and PsbR proteins, found in the green plant lineage. These proteins serve to stabilize the Mn4CaO5 cluster and optimize oxygen evolution at physiological calcium and chloride concentrations. The mechanisms used to perform these functions, however, remain poorly understood. Recently, important new findings have significantly advanced our understanding of the structures, locations and functions of these important subunits. We will discuss the biochemical, structural and genetic studies that have been used to elucidate the roles played by these proteins within the photosystem and their locations within the photosynthetic complex. Additionally, we will examine open questions needing to be addressed to provide a coherent picture of the role of these components within the photosystem

Usefulness of Ultrahigh Resolution Microstructural Studies for Understanding Localized Corrosion Behavior of Al Alloys

The corrosion behavior of different tempers of two aluminum alloys, AA7050 and an experimental Al–Mg–Cu–Si alloy, was studied in NaCl solution by anodic polarization and scanning electron microscopy and was correlated with differences in the microstructure. Potentiodynamic polarization experiments were performed on samples from the exact sheets used by others to study the microstructure evolution during the early stages of the precipitation sequence by high-resolution characterization tools [i.e., high-resolution transmission electron microscopy and atom probe tomography (APT)]. The usefulness of information from these state-of-the-art tools to lead to a better understanding about the effects of nanoscale segregation on localized corrosion of aluminum alloys is discussed. APT was able to provide information about the composition of the solid solution matrix region between the fine-scale hardening particles, which is not possible by any other technique. Some of the changes in corrosion behavior, e.g., the breakdown potentials, with temper could be rationalized based on changes in the matrix composition. The formation of corrosion-susceptible surface layers on as-polished AA7050 depended on the predominant type of hardening particle. The lack of detailed knowledge of the grain boundary region limited the applicability of the microstructural information generated by previous studies for understanding intergranular corrosion

Storage and Release of Soluble Hexavalent Chromium from Chromate Conversion Coatings on Al Alloys Kinetics of Release

The release of chromate ions from chromate conversion coatings (CCCs) on Al alloys was studied, and the effect of aging of CCCs on the chromate release kinetics was investigated. Chromate release from CCCs into aqueous solutions was monitored by measuring the change in the chromate concentration in solution using UV-visible spectroscopy. Heat-treatment of the CCC greatly reduced the chromate release rate. The chromate release rate also decreased with increasing aging time at room temperature. A diffusion-control model was proposed based on the notion that the CCC in an aqueous solution is a porous, two-phase structure consisting of a solid phase with adsorbed Cr(VI) species that is in local Langmuir-type equilibrium with an interpenetrating solution phase. This model results in a concentration gradient of soluble Cr(VI) in the solution phase of the CCC as chromate is released. The concentration and diffusion coefficients of soluble Cr(VI) in CCC were estimated. The estimated diffusion coefficient tended to decrease with aging time, suggesting that the CCC is modified with aging time.This work was supported under Air Force Office of Scientific Research Multidisciplinary University Research Initiative contract no. F49620-96-1-0479

Critical evaluation of the American Joint Commission on Cancer (AJCC) 8th edition staging system for patients with Hepatocellular Carcinoma (HCC): A Surveillance, Epidemiology, End Results (SEER) analysis

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142939/1/jso24908.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/142939/2/jso24908_am.pd

Nonlinear equation for curved stationary flames

A nonlinear equation describing curved stationary flames with arbitrary gas expansion $\theta = \rho_{{\rm fuel}}/\rho_{{\rm burnt}}$, subject to the Landau-Darrieus instability, is obtained in a closed form without an assumption of weak nonlinearity. It is proved that in the scope of the asymptotic expansion for $\theta \to 1,$ the new equation gives the true solution to the problem of stationary flame propagation with the accuracy of the sixth order in $\theta - 1.$ In particular, it reproduces the stationary version of the well-known Sivashinsky equation at the second order corresponding to the approximation of zero vorticity production. At higher orders, the new equation describes influence of the vorticity drift behind the flame front on the front structure. Its asymptotic expansion is carried out explicitly, and the resulting equation is solved analytically at the third order. For arbitrary values of $\theta,$ the highly nonlinear regime of fast flow burning is investigated, for which case a large flame velocity expansion of the nonlinear equation is proposed.Comment: 29 pages 4 figures LaTe

Diffusive Domain Coarsening : Early Time Dynamics and Finite-Size Effects

We study diffusive dynamics of phase separation in a binary mixture, following critical quench, both in spatial dimensions $d=2$ and $d=3$. Particular focus in this work is to obtain information about effects of system size and correction to the growth law via appropriate application of finite-size scaling method to the results obtained from Kawasaki exchange Monte Carlo simulation of Ising model. Observations of only weak size effects and very small correction to scaling in the growth law are significant. The methods used in this work and information thus gathered will be of paramount importance in the study of kinetics of phase separation in fluids and other problems of growing length scale. We also provide detailed discussion on standard methods of understanding simulation results which may lead to inappropriate conclusions.Comment: 11 pages, 12 figure