Good for learning, bad for motivation? A meta-analysis on the effects of computer-supported collaboration scripts

Scripting computer-supported collaborative learning has been shown to greatly enhance learning, but is often criticized for hindering learners’ agency and thus undermining learners’ motivation. Beyond that, what makes some CSCL scripts particularly effective for learning is still a conundrum. This meta-analysis synthesizes the results of 53 primary studies that experimentally compared the effect of learning with a CSCL script to unguided collaborative learning on at least one of the variables motivation, domain learning, and collaboration skills. Overall, 5616 learners enrolled in K-12, higher education, or professional development participated in the included studies. The results of a random-effects meta-analysis show that learning with CSCL scripts leads to a non-significant positive effect on motivation (Hedges’ g = 0.13), a small positive effect (Hedges’ g = 0.24) on domain learning and a medium positive effect (Hedges’ g = 0.72) on collaboration skills. Additionally, the meta-analysis shows how scaffolding single particular collaborative activities and scaffolding a combination of collaborative activities affects the effectiveness of CSCL scripts and that synergistic or differentiated scaffolding is hard to achieve. This meta-analysis offers the first counterevidence against the widespread criticism that CSCL scripts have negative motivational effects. Furthermore, the findings can be taken as evidence for the robustness of the positive effects on domain learning and collaboration skills

Dynamic subcompartmentalization of the mitochondrial inner membrane

The inner membrane of mitochondria is organized in two morphologically distinct domains, the inner boundary membrane (IBM) and the cristae membrane (CM), which are connected by narrow, tubular cristae junctions. The protein composition of these domains, their dynamics, and their biogenesis and maintenance are poorly understood at the molecular level. We have used quantitative immunoelectron microscopy to determine the distribution of a collection of representative proteins in yeast mitochondria belonging to seven major processes: oxidative phosphorylation, protein translocation, metabolite exchange, mitochondrial morphology, protein translation, iron–sulfur biogenesis, and protein degradation. We show that proteins are distributed in an uneven, yet not exclusive, manner between IBM and CM. The individual distributions reflect the physiological functions of proteins. Moreover, proteins can redistribute between the domains upon changes of the physiological state of the cell. Impairing assembly of complex III affects the distribution of partially assembled subunits. We propose a model for the generation of this dynamic subcompartmentalization of the mitochondrial inner membrane

Size-Dependent Variability in Flow and Viscoelastic Behavior of Levan Produced by Gluconobacter albidus TMW 2.1191

Levan is a fructan-type exopolysaccharide which is produced by many microbes from sucrose via extracellular levansucrases. The hydrocolloid properties of levan depend on its molecular weight, while it is unknown why and to what extent levan is functionally diverse depending on ist size. The aim of our study was to gain deeper insight into the size-dependent functional variability of levan. For this purpose, levans of different sizes were produced using the water kefir isolate Gluconobacter albidus TMW 2.1191 and subsequently rheologically characterized. Three levan types could be identified, which are similarly branched, but differ significantly in their molecular size and rheological properties. The smallest levan (10$^{8}$ Da) produced at pH ≥ 4.5 were shear-thinning, and the levan produced at pH 5.0 showed a gel-like behavior at 5% (w/v). A third (intermediate) levan variant was obtained through production in buffers at pH 4.0 and exhibited the properties of a viscoelastic fluid up to concentrations of 15% (w/v). Our study reveals that the rheological properties of levan are determined by its size and polydispersity, rather than by the amount of levan used or the structural composition

Comparative study of probabilistic modeling approaches for chloride ingress in concrete structures with macro‐cracks

Probabilistic service life analyses for assessing the risk of chloride-induced corrosion in uncracked concrete are often realized using the well-known chloride ingress model of the fib Model Code for Service Life Design. In practice, however, concrete includes cracks, which alter the resistance of the concrete against the chloride ingress. In the past, different approaches to account for preexisting cracks, were developed. In this study, these modeling approaches are summarized and compared in context of the probabilistic service life prognosis. Furthermore, a new approach to account for cracks by theoretically adapting the convection zone is presented. This study demonstrates that the choice of model extension to account for cracks vastly influences the prediction results and shows the limits of application of the current extensions

Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast

The MDM31 and MDM32 genes are required for normal distribution and morphology of mitochondria in the yeast Saccharomyces cerevisiae. They encode two related proteins located in distinct protein complexes in the mitochondrial inner membrane. Cells lacking Mdm31 and Mdm32 harbor giant spherical mitochondria with highly aberrant internal structure. Mitochondrial DNA (mtDNA) is instable in the mutants, mtDNA nucleoids are disorganized, and their association with Mmm1-containing complexes in the outer membrane is abolished. Mutant mitochondria are largely immotile, resulting in a mitochondrial inheritance defect. Deletion of either one of the MDM31 and MDM32 genes is synthetically lethal with deletion of either one of the MMM1, MMM2, MDM10, and MDM12 genes, which encode outer membrane proteins involved in mitochondrial morphogenesis and mtDNA inheritance. We propose that Mdm31 and Mdm32 cooperate with Mmm1, Mmm2, Mdm10, and Mdm12 in maintenance of mitochondrial morphology and mtDNA

A Novel Multi Slit X-Ray Backscatter Camera Based on Synthetic Aperture Focusing

A special slit collimator was developed earlier for fast acquisition of X-ray back scatter images. The design was based on a twisted slit design (ruled surfaces) in a Tungsten block to acquire backscatter images. The comparison with alternative techniques as the flying spot and the coded aperture pin hole technique could not prove the expected higher contrast sensitivity. In analogy to the coded aperture technique, a novel multi slit camera was designed and tested. Several twisted slits were parallelly arranged in a metal block. The CAD design of different multi-slit cameras was evaluated and optimized by the computer simulation packages aRTist and McRay. The camera projects a set of equal images per slit to the digital detector array, which are overlaying each other. Afterwards, the aperture is corrected based on a deconvolution algorithm to focus the overlaying projections into a single representation of the object. Furthermore, a correction of the geometrical distortions due to the slit geometry is performed. The expected increase of the contrast-to-noise ratio is proportional to the square root of the number of parallel slits in the camera. However, additional noise has to be considered originating from the deconvolution operation. The slit design, functional principle, and the expected limits of this technique will be discussed