Functional Interaction of Nuclear Domain 10 and Its Components with Cytomegalovirus after Infections: Cross-Species Host Cells versus Native Cells

Species-specificity is one of the major characteristics of cytomegaloviruses (CMVs) and is the primary reason for the lack of a mouse model for the direct infection of human CMV (HCMV). It has been determined that CMV cross-species infections are blocked at the post-entry level by intrinsic cellular defense mechanisms, but few details are known. It is important to explore how CMVs interact with the subnuclear structure of the cross-species host cell. In our present study, we discovered that nuclear domain 10 (ND10) of human cells was not disrupted by murine CMV (MCMV) and that the ND10 of mouse cells was not disrupted by HCMV, although the ND10-disrupting protein, immediate-early protein 1 (IE1), also colocalized with ND10 in cross-species infections. In addition, we found that the UL131-repaired HCMV strain AD169 (vDW215-BADrUL131) can infect mouse cells to produce immediate-early (IE) and early (E) proteins but that neither DNA replication nor viral particles were detectable in mouse cells. Unrepaired AD169 can express IE1 only in mouse cells. In both HCMV-infected mouse cells and MCMV-infected human cells, the knocking-down of ND10 components (PML, Daxx, and SP100) resulted in significantly increased viral-protein production. Our observations provide evidence to support our hypothesis that ND10 and ND10 components might be important defensive factors against the CMV cross-species infection

b-tagging in DELPHI at LEP

Abstract: The standard method used for tagging b-hadrons in the DELPHI experiment at the CERN LEP Collider is discussed in detail. The main ingredient of b-tagging is the impact parameters of tracks, which relies mostly on the vertex detector. Additional information, such as the mass of particles associated to a secondary vertex, significantly improves the selection efficiency and the background suppression. The paper describes various discriminating variables used for the tagging and the procedure of their combination. In addition, applications of b-tagging to some physics analyses, which depend crucially on the performance and reliability of b-tagging, are described briefly

Measurement of the Tau Lepton Polarisation at LEP2

A first measurement of the average polarisation P_tau of tau leptons produced in e+e- annihilation at energies significantly above the Z resonance is presented. The polarisation is determined from the kinematic spectra of tau hadronic decays. The measured value P_tau = -0.164 +/- 0.125 is consistent with the Standard Model prediction for the mean LEP energy of 197 GeV.A first measurement of the average polarisation Pτ of tau leptons produced in e + e − annihilation at energies significantly above the Z resonance is presented. The polarisation is determined from the kinematic spectra of tau hadronic decays. The measured value Pτ=−0.164±0.125 is consistent with the Standard Model prediction for the mean LEP energy of 197 GeV.A first measurement of the average polarisation P_tau of tau leptons produced in e+e- annihilation at energies significantly above the Z resonance is presented. The polarisation is determined from the kinematic spectra of tau hadronic decays. The measured value P_tau = -0.164 +/- 0.125 is consistent with the Standard Model prediction for the mean LEP energy of 197 GeV

7th Drug hypersensitivity meeting: part two

No abstract availabl

Intramolecular 1,5- versus 1,6-hydrogen abstraction reaction promoted by alkoxy radicals in carbohydrate models

3 pages, 1 table, 2 schemes.-- PMID: 12027657 [PubMed].-- Printed version published May 30, 2002.-- Supporting information available at: http://pubs.acs.org/doi/suppl/10.1021/ol025981uErratum to this paper published in: Org. Lett. 4(19): 3337 (2002), http://dx.doi.org/10.1021/ol020134cThe alkoxy radical generated by reaction of 3,7-anhydro-2-deoxyoctitols with (diacetoxyiodo)benzene (DIB) and iodine abstracts regioselectively either the proton at C7 or that at C4 depending on the electronegativity of the substituent at C4. The correct election of this substituent can switch the reaction to give 2,9-dioxabicyclo[3.3.1]nonane or hexahydro-2H-furo[3,2-b]pyran ring systems.This work was supported by the Investigation Programs BQU2000-0650 and BQU2001-1665 of the Dirección General de Investigación Científica y Técnica, Spain. A.J.H. and I.P.-M. thank the Ministerio de Educación y Ciencia, Spain, and the Program I3P-CSIC, respectively, for fellowships

Intramolecular hydrogen abstraction promoted by N-radicals: synthesis of chiral 7-oxa-2-azabicyclo[2.2.1]heptane and 8-oxa-6-azabicyclo[3.2.1]octane ring systems

4 pages, 1 table, 1 scheme.Homochiral 7-oxa-2-azabicyclo[2.2.1]heptane and 8-oxa-6-azabicyclo[3.2.1]octane ring systems can be synthesized by reaction of specifically protected phosphoramidate derivatives of carbohydrates with (diacetoxyiodo)benzene or iodosylbenzene and iodine. The reaction mechanism goes through homolytic fragmentation of a hypothetical iodoamide intermediate. The N-radicals so generated participate in an intramolecular hydrogen abstraction reaction (IHA) to give the aforementioned bicycles.This work was supported by Investigation Programme PB96-1461 of the Direction General de Investigación Científica y Técnica. A.J.H. thanks the Ministerio de Educación y Ciencia, Spain, for a fellowship

Reagents with Hypervalent Iodine: Formation of Convenient Chiral Synthetic Intermediates by Fragmentation of Carbohydrate Anomeric Alkoxy Radicals

3 pages, 1 table.Cleavage of the C1-C2 bond in carbohydrates is achieved via the related alkoxy radicals by adding (diacetoxyiodo)benzene (DIB) to the substrate in the presence of iodine [Eq.(a)]. The course of the reaction is independent of the protecting groups R, the ring size, and the configuration of the substrate at C2. The cleavage products are valuable chiral building blocks.This work was supported by the Investigation Programme no. PB87-0406 of the Dirección General de Investigación Cientifica y Técnica

Reagents with Hypervalent Iodine: Formation of Convenient Chiral Synthetic Intermediates by Fragmentation of Carbohydrate Anomeric Alkoxy Radicals

3 pages, 1 table.Cleavage of the C1-C2 bond in carbohydrates is achieved via the related alkoxy radicals by adding (diacetoxyiodo)benzene (DIB) to the substrate in the presence of iodine [Eq.(a)]. The course of the reaction is independent of the protecting groups R, the ring size, and the configuration of the substrate at C2. The cleavage products are valuable chiral building blocks.This work was supported by the Investigation Programme no. PB87-0406 of the Dirección General de Investigación Cientifica y Técnica

Organoselenium reagents in the tandem β-fragmentation-cyclization of carbohydrate anomeric alkoxy radicals

4 pages, 1 table, 1 scheme.Carbohydrates possessing a suitably positioned hydroxyl group in the presence of organoselenium reagents and iodine under an argon atmosphere undergo intramolecular β-fragmentation-cyclization reaction to give aldofuranose and aldopyranose forms of carbohydrates, specifically.This work was supported by Investigation Programme No. PB90-0083 of the Dirección General de Investigación Científica y Técnica. We thank the Dirección General de Universidades e Investigación de Canarias for a fellowship granted to R.L. Dorta