Weak Interactions between Salmonella enterica FlhB and Other Flagellar Export Apparatus Proteins Govern Type III Secretion Dynamics

The bacterial flagellum contains its own type III secretion apparatus that coordinates protein export with assembly at the distal end. While many interactions among export apparatus proteins have been reported, few have been examined with respect to the differential affinities and dynamic relationships that must govern the mechanism of export. FlhB, an integral membrane protein, plays critical roles in both export and the substrate specificity switching that occurs upon hook completion. Reported herein is the quantitative characterization of interactions between the cytoplasmic domain of FlhB (FlhBC) and other export apparatus proteins including FliK, FlhAC and FliI. FliK and FlhAC bound with micromolar affinity. KD for FliI binding in the absence of ATP was 84 nM. ATP-induced oligomerization of FliI induced kinetic changes, stimulating fast-on, fast-off binding and lowering affinity. Full length FlhB purified under solubilizing, nondenaturing conditions formed a stable dimer via its transmembrane domain and stably bound FliH. Together, the present results support the previously hypothesized central role of FlhB and elucidate the dynamics of protein-protein interactions in type III secretion

The Transit Light Curve Project. VI. Three Transits of the Exoplanet TrES-2

Of the nearby transiting exoplanets that are amenable to detailed study, TrES-2 is both the most massive and has the largest impact parameter. We present z-band photometry of three transits of TrES-2. We improve upon the estimates of the planetary, stellar, and orbital parameters, in conjunction with the spectroscopic analysis of the host star by Sozzetti and co-workers. We find the planetary radius to be 1.222 +/- 0.038 R_Jup and the stellar radius to be 1.003 +/- 0.027 R_Sun. The quoted uncertainties include the systematic error due to the uncertainty in the stellar mass (0.980 +/- 0.062 M_Sun). The timings of the transits have an accuracy of 25s and are consistent with a uniform period, thus providing a baseline for future observations with the NASA Kepler satellite, whose field of view will include TrES-2.Comment: 15 pages, including 2 figures, accepted Ap

Surficial Redistribution of Fallout 131iodine in a Small Temperate Catchment

Isotopes of iodine play significant environmental roles, including a limiting micronutrient (127I), an acute radiotoxin (131I), and a geochemical tracer (129I). But the cycling of iodine through terrestrial ecosystems is poorly understood, due to its complex environmental chemistry and low natural abundance. To better understand iodine transport and fate in a terrestrial ecosystem, we traced fallout 131iodine throughout a small temperate catchment following contamination by the 11 March 2011 failure of the Fukushima Daiichi nuclear power facility. We find that radioiodine fallout is actively and efficiently scavenged by the soil system, where it is continuously focused to surface soils over a period of weeks following deposition. Mobilization of historic (pre-Fukushima) 137cesium observed concurrently in these soils suggests that the focusing of iodine to surface soils may be biologically mediated. Atmospherically deposited iodine is subsequently redistributed from the soil system via fluvial processes in a manner analogous to that of the particle-reactive tracer 7beryllium, a consequence of the radionuclides’ shared sorption affinity for fine, particulate organic matter. These processes of surficial redistribution create iodine hotspots in the terrestrial environment where fine, particulate organic matter accumulates, and in this manner regulate the delivery of iodine nutrients and toxins alike from small catchments to larger river systems, lakes and estuaries

The Prograde Orbit of Exoplanet TrES-2b

We monitored the Doppler shift of the G0V star TrES-2 throughout a transit of its giant planet. The anomalous Doppler shift due to stellar rotation (the Rossiter-McLaughlin effect) is discernible in the data, with a signal-to-noise ratio of 2.9, even though the star is a slow rotator. By modeling this effect we find that the planet's trajectory across the face of the star is tilted by -9 +/- 12 degrees relative to the projected stellar equator. With 98% confidence, the orbit is prograde.Comment: ApJ, in press [15 pages

Improving Stellar and Planetary Parameters of Transiting Planet Systems: The Case of TrES-2

We report on a spectroscopic determination of the atmospheric parameters and chemical abundance of the parent star of the recently discovered transiting planet TrES-2. A detailed LTE analysis of a set of Fe I and Fe II lines from our Keck spectra yields T_(eff) = 5850 ± 50 K, log g = 4.4 ± 0.1, and [Fe/H] = -0.15 ± 0.10. Several independent checks (e.g., additional spectroscopy, line-depth ratios) confirm the reliability of our spectroscopic T_(eff) estimate. The mass and radius of the star, needed to determine the properties of the planet, are traditionally inferred by comparison with stellar evolution models using T_(eff) and some measure of the stellar luminosity, such as the spectroscopic surface gravity. We apply here a new method in which we use instead of log g the normalized separation a/R_* (related to the stellar density), directly measurabele from the light curves of transiting planets with much greater precision. With the a/R_* value from the light-curve analysis of Holman and coworkers and our T_(eff) estimate, we obtain M_* = 0.980 ± 0.062 M_☉ and R_* = 1.000^(+0.036)_(-0.033) R_☉, and an evolutionary age of 5.1^(+2.7)_(-2.3) Gyr, in good agreement with other constraints (Ca II H and K line cores, lithium abundance, and rotation). The new stellar parameters yield improved values for the planetary mass and radius of M_p = 1.198 ± 0.053 M_J and R_p = 1.220^(+0.045)_(-0.042) R_J, confirming that TrES-2 is the most massive among the currently known nearby (d ≲ 300 pc) transiting hot Jupiters. The surface gravity of the planet, log g_p = 3.299 ± 0.016, can be derived independently of the knowledge of the stellar parameters (i.e., directly from observations), and with a very high precision rivaling that of the best known double-lined eclipsing binaries

Precise Radius Estimates for the Exoplanets WASP-1b and WASP-2b

We present precise z-band photometric time series spanning times of transit of the two exoplanets recently discovered by the SuperWASP collaboration. We find planetary radii of 1.44 +/- 0.08 R_J and 1.04 +/- 0.06 R_J for WASP-1b and WASP-2b, respectively. These error estimates include both random errors in the photometry and also the uncertainty in the stellar masses. Our results are 5 times more precise than the values derived from the discovery data alone. Our measurement of the radius of WASP-2b agrees with previously published models of hot Jupiters that include both a 20-M_Earth core of solid material and the effects of stellar insolation. In contrast, we find that the models cannot account for the large size of WASP-1b, even if the planet has no core. Thus, we add WASP-1b to the growing list of hot Jupiters that are larger than expected. This suggests that ``inflated'' hot Jupiters are more common than previously thought, and that any purported explanations involving highly unusual circumstances are disfavored.Comment: 15 pages, 2 figures, submitted to Ap

TrES-2: The First Transiting Planet in the Kepler Field

We announce the discovery of the second transiting hot Jupiter discovered by the Trans-atlantic Exoplanet Survey. The planet, which we dub TrES-2, orbits the nearby star GSC 03549-02811 every 2.47063 days. From high-resolution spectra, we determine that the star has T_eff = 5960 +/- 100 K and log(g) = 4.4 +/- 0.2, implying a spectral type of G0V and a mass of 1.08 +0.11/-0.05 M_sun. High-precision radial-velocity measurements confirm a sinusoidal variation with the period and phase predicted by the photometry, and rule out the presence of line-bisector variations that would indicate that the spectroscopic orbit is spurious. We estimate a planetary mass of 1.28 +0.09/-0.04 M_Jup. We model B, r, R, and I photometric timeseries of the 1.4%-deep transits and find a planetary radius of 1.24 +0.09/-0.06 R_Jup. This planet lies within the field of view of the NASA Kepler mission, ensuring that hundreds of upcoming transits will be monitored with exquisite precision and permitting a host of unprecedented investigations.Comment: Accepted for publication in ApJL. 15 pages, 2 figure

Extracorporeal membrane oxygenation support after the Fontan operation

ObjectiveExtracorporeal membrane oxygenation has been used to support children with cardiac failure after the Fontan operation. Mortality is high, and causes of mortality remain unclear. We evaluated the in-hospital mortality and factors associated with mortality in these patients.MethodsExtracorporeal Life Support Organization registry data on patients requiring extracorporeal membrane oxygenation after the Fontan operation from 1987 to 2009 were retrospectively analyzed. Demographics and extracorporeal membrane oxygenation data were compared for survivors and nonsurvivors. A multivariable logistic regression model was used to identify factors associated with mortality.ResultsOf 230 patients, 81 (35%) survived to hospital discharge. Cardiopulmonary resuscitation was more frequent (34% vs 17%, P = .04), and median fraction of inspired oxygen concentration was higher (1 [confidence interval, 0.9–1.0] vs 0.9 [confidence interval, 0.8–1.0], P = .03) before extracorporeal membrane oxygenation in nonsurvivors compared with survivors. Extracorporeal membrane oxygenation duration and incidence of complications, including surgical bleeding, neurologic injury, renal failure, inotrope use on extracorporeal membrane oxygenation, and bloodstream infection, were higher in nonsurvivors compared with survivors (P < .05 for all). In a multivariable model, neurologic injury (odds ratio, 5.18; 95% confidence interval, 1.97–13.61), surgical bleeding (odds ratio, 2.36; 95% confidence interval, 1.22–4.56), and renal failure (odds ratio, 2.81; 95% confidence interval, 1.41–5.59) increased mortality. Extracorporeal membrane oxygenation duration of more than 65 hours to 119 hours (odds ratio, 0.33; 95% confidence interval, 0.14–0.76) was associated with decreased mortality.ConclusionsCardiac failure requiring extracorporeal membrane oxygenation after the Fontan operation is associated with high mortality. Complications during extracorporeal membrane oxygenation support increase mortality odds. Prompt correction of surgical bleeding when possible may improve survival

A New Spectroscopic and Photometric Analysis of the Transiting Planet Systems TrES-3 and TrES-4

We report new spectroscopic and photometric observations of the parent stars of the recently discovered transiting planets TrES-3 and TrES-4. A detailed abundance analysis based on high-resolution spectra yields [Fe/H] = –0.19 ± 0.08, T_(eff) = 5650 ± 75 K, and log g = 4.4 ± 0.1 for TrES-3, and [Fe/H] = +0.14 ± 0.09, T_(eff) = 6200 ± 75 K, and log g = 4.0 ± 0.1 for TrES-4. The accuracy of the effective temperatures is supported by a number of independent consistency checks. The spectroscopic orbital solution for TrES-3 is improved with our new radial velocity measurements of that system, as are the light-curve parameters for both systems based on newly acquired photometry for TrES-3 and a reanalysis of existing photometry for TrES-4. We have redetermined the stellar parameters taking advantage of the strong constraint provided by the light curves in the form of the normalized separation a/R_* (related to the stellar density) in conjunction with our new temperatures and metallicities. The masses and radii we derive are M_* = 0.928^(+0.028)_(–0.048) M_⊙, R_* = 0.829^(+0.015)_(–0.022) R_⊙, and M_* = 1.404^(+0.066)_(–0.134) M_⊙, R_* = 1.846^(+0.096)_(–0.087) R_⊙ for TrES-3 and TrES-4, respectively. With these revised stellar parameters, we obtain improved values for the planetary masses and radii. We find M_p = 1.910^(+0.075)_(–0.080) M_(Jup), R_p = 1.336^(+0.031)_(–0.036) R_(Jup) for TrES-3, and M_p = 0.925 ± 0.082 M_(Jup), R_p = 1.783^(+0.093)_(–0.086) R_(Jup) for TrES-4. We confirm TrES-4 as the planet with the largest radius among the currently known transiting hot Jupiters

2006 SQ372: A Likely Long-Period Comet from the Inner Oort Cloud

We report the discovery of a minor planet (2006 SQ372) on an orbit with a perihelion of 24 AU and a semimajor axis of 796 AU. Dynamical simulations show that this is a transient orbit and is unstable on a timescale of 200 Myrs. Falling near the upper semimajor axis range of the scattered disk and the lower semimajor axis range of the Oort Cloud, previous membership in either class is possible. By modeling the production of similar orbits from the Oort Cloud as well as from the scattered disk, we find that the Oort Cloud produces 16 times as many objects on SQ372-like orbits as the scattered disk. Given this result, we believe this to be the most distant long-period comet ever discovered. Furthermore, our simulation results also indicate that 2000 OO67 has had a similar dynamical history. Unaffected by the "Jupiter-Saturn Barrier," these two objects are most likely long-period comets from the inner Oort Cloud