Design and implementation of a modulating test plant to assess the performance of innovative cross-flow heat recovery units for air conditioning system: Preliminary results

Nowadays global warming has increased consciousness of the dangers of energy wastefulness: in the last 50 years the temperature of the Earth's surface rose by approximately 1 °C. The building sector is responsible for a very high percentage of world carbon emissions and with the increasing of the request for comfort, heating, ventilation and air conditioning, buildings energy consumption is rapidly growing. Consequently, it appears fundamental the role played by the improvement of buildings energy performance within global policies of emissions reduction. In this context an increasing attention is given to the energy waste reduction in tertiary sector: bars, offices, restaurants, meetings, shops, school buildings, gyms and in general in the buildings in which the minimization of the energy dissipation is requested. The present study is part of the NANOFANCOIL project (POR-FESR 2014-2020): one of the objectives was the design and implementation of a modulating test plant to assess the performance of innovative cross-flow heat recovery units for air conditioning system. This experimental setup was mainly composed by two climatic chambers that enabled to simulate the environmental conditions of interest. The temperature could vary from -20°C to 0°C and from 10°C to 30°C for the cold and the hot chamber, respectively (i.e. the outdoor and the indoor environment). Moreover, the climatic chamber that simulates the indoor environment could be controlled also in terms of humidity thanks to a steam humidifier that guaranteed 5 kg/h of vapour. Preliminary results about the heat transfer behaviour of a cross-flow heat recovery unit (air-to-air) for controlled mechanical ventilation systems that employs mini-channels in order to increase performance and reduce size and costs are presented here

Eficiencia biológica de biotipos lecheros de primera lactancia en sistemas a pastoreo

Marini, P.R.; Castro, R.; Frana, E.; Di Masso, R.J.: Eficiencia biológica de biotipos lecheros de primera lactancia en sistemas a pastoreo. Rev. vet. 26: 2, 136-142, 2015

(n,xn) cross section measurements for Y-89 foils used as detectors for high energy neutron measurements in the deeply subcritical assembly “QUINTA”

Study of the deep subcritical systems (QUINTA) using relativistic beams is performed within the project “Energy and Transmutation of Radioactive Wastes” (E&T – RAW). The experiment assembly was irradiated by deuteron/proton beam (Dubna NUCLOTRON). We calculated the neutron energy spectrum inside the whole assembly by using threshold energy (n,xn) reactions in yttrium (Y-89) foils. There are almost no experimental cross section data for those reactions. New Y-89(n,xn) cross section measurements were carried out at The Svedberg laboratory (TSL) in Uppsala, Sweden in 2015. In this paper we present preliminary results of those experiments

Cleavage of the SARS Coronavirus Spike Glycoprotein by Airway Proteases Enhances Virus Entry into Human Bronchial Epithelial Cells In Vitro

Background: Entry of enveloped viruses into host cells requires the activation of viral envelope glycoproteins through cleavage by either intracellular or extracellular proteases. In order to gain insight into the molecular basis of protease cleavage and its impact on the efficiency of viral entry, we investigated the susceptibility of a recombinant native full-length S-protein trimer (triSpike) of the severe acute respiratory syndrome coronavirus (SARS-CoV) to cleavage by various airway proteases. Methodology/Principal Findings: Purified triSpike proteins were readily cleaved in vitro by three different airway proteases: trypsin, plasmin and TMPRSS11a. High Performance Liquid Chromatography (HPLC) and amino acid sequencing analyses identified two arginine residues (R667 and R797) as potential protease cleavage site(s). The effect of protease-dependent enhancement of SARS-CoV infection was demonstrated with ACE2 expressing human bronchial epithelial cells 16HBE. Airway proteases regulate the infectivity of SARS-CoV in a fashion dependent on previous receptor binding. The role of arginine residues was further shown with mutant constructs (R667A, R797A or R797AR667A). Mutation of R667 or R797 did not affect the expression of S-protein but resulted in a differential efficacy of pseudotyping into SARS-CoVpp. The R667A SARS-CoVpp mutant exhibited a lack of virus entry enhancement following protease treatment. Conclusions/Significance: These results suggest that SARS S-protein is susceptible to airway protease cleavage and, furthermore, that protease mediated enhancement of virus entry depends on specific conformation of SARS S-protein upon ACE2 binding. These data have direct implications for the cell entry mechanism of SARS-CoV along the respiratory system and, furthermore expand the possibility of identifying potential therapeutic agents against SARS-CoV. © 2009 Kam et al.published_or_final_versio

Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner

Enveloped viruses need to fuse with a host cell membrane in order to deliver their genome into the host cell. While some viruses fuse with the plasma membrane, many viruses are endocytosed prior to fusion. Specific cues in the endosomal microenvironment induce conformational changes in the viral fusion proteins leading to viral and host membrane fusion. In the present study we investigated the entry of coronaviruses (CoVs). Using siRNA gene silencing, we found that proteins known to be important for late endosomal maturation and endosome-lysosome fusion profoundly promote infection of cells with mouse hepatitis coronavirus (MHV). Using recombinant MHVs expressing reporter genes as well as a novel, replication-independent fusion assay we confirmed the importance of clathrin-mediated endocytosis and demonstrated that trafficking of MHV to lysosomes is required for fusion and productive entry to occur. Nevertheless, MHV was shown to be less sensitive to perturbation of endosomal pH than vesicular stomatitis virus and influenza A virus, which fuse in early and late endosomes, respectively. Our results indicate that entry of MHV depends on proteolytic processing of its fusion protein S by lysosomal proteases. Fusion of MHV was severely inhibited by a pan-lysosomal protease inhibitor, while trafficking of MHV to lysosomes and processing by lysosomal proteases was no longer required when a furin cleavage site was introduced in the S protein immediately upstream of the fusion peptide. Also entry of feline CoV was shown to depend on trafficking to lysosomes and processing by lysosomal proteases. In contrast, MERS-CoV, which contains a minimal furin cleavage site just upstream of the fusion peptide, was negatively affected by inhibition of furin, but not of lysosomal proteases. We conclude that a proteolytic cleavage site in the CoV S protein directly upstream of the fusion peptide is an essential determinant of the intracellular site of fusion

The influence of the wall temperature on the flow from the floor convector (experimental results)

This article describes the measurement of the influnce of the wall temperature on flow from the floor convector. The measurement is realized in an open space laboratory inlet and outlet is visulized using PIV technique. Two temperatures 19.8 °C and 12.5 °C are set at the wall to evaluate the influnce of the wall cooling

The influence of the wall temperature on the flow from the floor convector (experimental results)

This article describes the measurement of the influnce of the wall temperature on flow from the floor convector. The measurement is realized in an open space laboratory inlet and outlet is visulized using PIV technique. Two temperatures 19.8 °C and 12.5 °C are set at the wall to evaluate the influnce of the wall cooling

The influence of the wall temperature on the flow from the floor convector (experimental results)

This article describes the measurement of the influnce of the wall temperature on flow from the floor convector. The measurement is realized in an open space laboratory inlet and outlet is visulized using PIV technique. Two temperatures 19.8 °C and 12.5 °C are set at the wall to evaluate the influnce of the wall cooling