THE IMPACT OF PROMOTERS AND CELL-LINE TYPES ON ALTERNATIVE SPLICING OF RECOMBINANT CONSTRUCTS

Alternative intron splicing is a process where introns are excised from pre-mRNA following different patterns. The consequence of it is a presence of several different forms of mature mRNAs differing in length and in content of the whole exons or their parts. Studies in the past decade lead to the view that a complex and extensively coupled network has evolved to coordinate the activities of gene expression pathway, which includes transcription, several pre-mRNA processing steps and the export of mature mRNA to the cytoplasm. Coupling of transcription to splicing was revealed in studies showing that transcription of pre-mRNA by different promoters can generate different alternatively spliced mRNAs. The main goal of this study was to determine the impact of different milk gene promoters on alternative splicing under in vitro conditions. Besides, we also investigated how different cell types influence the splice site selection. With this experiment, we confirmed our predictions that promoter structure and cell-type have an impact on alternative splicing. Differences were observed in quantity of transcripts, as well as in splice-site selection

(1737) Proposal to conserve the name Heliosperma against Ixoca (Caryophyllaceae, Sileneae)

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146904/1/tax25065663.pd

KANDIDATNI GENI ZA KLAONIČKA SVOJSTVA KOD SVINJA

Rapid development of DNA research techniques in the last few decades has enabled identification of genes that underlie genetic variation of production traits observed in livestock species. Identification of these genes is expected to allow more efficient selection with employment of genetic markers and to yield more accurate insight into the physiology of the corresponding traits. Majority of production traits are polygenic and the first step in the determination of their genetic background is in searching for so called candidate genes with an impact on a defined trait. Production traits in pigs, like growth and carcass characteristics, play an important role in pig breeding and selection. Like many other economically important traits in farm animals they are determined by an unknown number of genes together with environmental factors. Meat quality assessment is based on measuring some phenotypic traits like intramuscular fat content, pH-value, electric conductivity, drip loss and color. The study of candidate genes, in connection with phenotypic effects, is an important tool to identify genes to be used in marker-assisted selection programs. This work is a review of some candidate genes for which it has been established that they have an important impact on carcass traits in pigs, like for example growth hormone gene complex, PPARGC1, pituitaryspecific transcription factor, melanocortin receptor and myogenin.Brzi razvoj tehnika za istraživanje DNA zadnjih desetljeća omogućio je identifikaciju gena koji su temelj genetske raznolikosti produktivnih svojstava uočenih kod doma_ih životinja. Identifikacija ovih gena može povećati uspješnosti selekcije temeljene na genskim markerima i dovesti do točnijeg razumijevanja fiziologije odgovarajućih svojstava. Većina proizvodnih svojstava svinja je određena većim brojem gena i prvi korak u određivanju njihove genske osnove je istraživanje tzv. kandidatnih gena i njihov učinak na određena svojstva. Proizvodna i klaonička svojstva kod svinja imaju veliku važnost u uzgoju i selekciji. Kao i mnoga druga ekonomski važna svojstva domaćih životinja, i ova svojstva su određena neutvrđenim brojem gena u interakciji s okolišnim čimbenicima. Intenzivna selekcija svinja na visoku mesnatost za posljedicu je imala narušavanje proizvodnih, kao i svojstava kakvoće mišičnog tkiva kod nekih pasmina. Sadržaj intramuskularne masti, profil masnih kiselina, pH-vrijednost, boja mesa, električna provodljivost i sposobnost vezanja vode svojstva su koja u znatnoj mjeri određuju kakvoću mišičnog tkiva svinja. Većina ovih svojstava genetski je uvjetovana, tako da je selekcijom moguće utjecati na njihovo poboljšanje. Proučavanje kandidatnih gena, povezano s fenotipskim učinkom, je značajno oruđe za određivanje gena koji će se koristiti u selekciji. U ovom radu dan je pregled nekih kandidatnih gena za koje je utvrđeno da imaju značajan utjecaj na klaonička svojstva svinja, kao što su kompleks gena za hormon rasta PPARGC1, hipofizno-specifični transkripcijski faktor, melanokortinski receptor i miogenin

Civil Society, Popular Protest, and Democracy in Latin America

This dissertation addresses the relationship between mobilized coalitions of movements and organizations emerging from civil society and the promotion of democracy. It offers a critique of major works in political theory that see in civil society the potential to transform democratic politics, primarily through the protection of civil society from the state in order to allow for the development of new identities and forms of sociability. The three main theoretical objections to these works involve their focus on state-civil society relations at the expense of economic factors, the presupposition that consensus is present in civil society, and the assumption that mobilized civil societies are fueled from the grassroots. Four recent cases of civil society mobilizations from Latin America, in Argentina, Costa Rica, Ecuador, and Bolivia, are presented to illustrate the deficiencies of current theoretical approaches to civil society. The case studies show the importance of material conditions and the framing of specific grievances in the formation of popular movements grounded in civil society

Notes on some rare Orobanche and Phelipanche species (Orobanchaceae) in Croatia

We report new floristic records of some rare Orobanche and Phelipanche species (Orobanchaceae) in Croatia. Orobanche salviae and O. alsatica are reported for the first time in 100 years, and O. laserpitii-sileris, and P. lavandulacea are rare species with only few localities known