Immunoreactivity of the 14F7 Mab (Raised against N-Glycolyl GM3 Ganglioside) as a Positive Prognostic Factor in Non-Small-Cell Lung Cancer

Lung carcinoma is the leading cause of cancer-related mortality worldwide. Therefore, numerous studies are focusing on the assessment of other biological and molecular prognostic factors in these tumors. We evaluated the relationship between 14F7 Mab reactivity, pathological features, DNA-content and S-phase fraction (SPF), and their impact in the survival of NSCLC patients. Hematoxylin and eosin staining and immunohistochemistry optical microscopy assays as well as DNA content and SPF measuring using flow cytometry were performed. The 14F7 reactivity was widely observed in NSCLC sections, no depending of the clinicopathological characteristics. We also obtained differences in the intensity of reaction with 14F7 as well as in the SPF between diploid and aneuploid carcinomas. Patients with diploid tumors showing higher SPF and 14F7 reaction joint to a low mitotic index displayed higher survival rates. Our results are in agreement with the assumption of the possible positive prognostic value of 14F7 staining in NSCLC

Immunohistochemical Reactivity of the 14F7 Monoclonal Antibody Raised against N-Glycolyl GM3 Ganglioside in Some Benign and Malignant Skin Neoplasms

The evaluation of 14F7 Mab (anti-N-glycolyl GM3 ganglioside) immunorecognition in normal skin, cutaneous malignant melanoma (CMM), and in lymph node metastases (LNM) has been previously reported. In this work we extended the study to benign (BMN) and dysplastic (DMN) melanocytic nevi, basal (BCC), and squamous cell carcinoma (SCC). Immunohistochemical assays with 14F7 followed by a biotinylated link universal and streptavidin-AP in normal and pathological tissues were made. No reaction of 14F7 in normal skin (0/10) as well as a low reactivity in BMN (2/11) and DMN (1/7) was detected. A limited staining in BCC (2/13) and in SCC (4/8) was also evidenced, while 14F7 Mab were mostly reactive in CMM (28/28) and in LNM (6/7). These results suggest that 14F7 reactivity could be closely related with the more aggressive biological behavior of CMM and also support the use of NeuGcGM3 as target for both passive and active melanoma immunotherapy

Uma alternativa para o fortalecimento da educação nutricional do aluno do curso de Licenciatura em Ensino de Química Industrial: Uma alternativa para o fortalecimento da educação nutricional

The object of the profession of the Graduate in Industrial Chemical Education, is conceived as a consciously organized process of educational influences aimed at the integral preparation of the competent worker, which takes place in the integration educational institution - labor entity - community. Within this humanist training, nutritional education is included, as a way of better health and quality of life. The present work aims to socialize the results of the incorporation into the curriculum of the subject Food Chemistry and its corresponding reflective workshops, aimed at strengthening nutrition education. Theoretical, empirical and statistical-mathematical research methods were applied. The results achieved in the implementation of the proposal show its feasibility and relevance, also revealing a great acceptance in the students, given its contribution to the training of the professional.El objeto de la profesión del Licenciado en Educación Química Industrial, se concibe como un proceso conscientemente organizado de influencias educativas dirigido a la preparación integral del obrero competente, que tiene lugar en la integración institución educativa - entidad laboral - comunidad. Dentro de esa formación humanista se incluye la educación nutricional, como vía de una mejor salud y calidad de vida. El presente trabajo tiene como objetivo socializar los resultados de la incorporación en el currículo de la asignatura Química de los Alimentos y sus correspondientes talleres reflexivos, dirigidos a fortalecer la educación nutricional. Se aplicaron métodos de investigación teóricos, empíricos y estadístico-matemáticos. Los resultados alcanzados en la implementación de la propuesta muestran su factibilidad y pertinencia, revelándose también una gran aceptación en los estudiantes, dada su contribución a la formación del profesional.O objeto da profissão do Bacharelado em Ensino de Química Industrial é concebido como um processo conscientemente organizado de influências educacionais visando à formação integral do trabalhador competente, que se dá na instituição de ensino - entidade trabalhista - integração comunitária. A educação nutricional está inserida nessa educação humanística, como forma de melhorar a saúde e a qualidade de vida. O objetivo deste trabalho é socializar os resultados da incorporação no currículo da disciplina de Química de Alimentos e suas correspondentes oficinas reflexivas, visando o fortalecimento da educação nutricional. Foram aplicados métodos de pesquisa teóricos, empíricos e estatístico-matemáticos. Os resultados alcançados na implementação da proposta evidenciam a sua viabilidade e relevância, revelando também uma grande aceitação por parte dos alunos, dada a sua contribuição para a formação profissional

Una alternativa para el fortalecimiento de la educación nutricional del estudiante de la carrera licenciatura en educación química industrial

The object of the profession of the Graduate in Industrial Chemical Education, is conceived as a consciously organized process of educational influences aimed at the integral preparation of the competent worker, which takes place in the integration educational institution -labor entity -community. Within this humanist training, nutritional education is included, as a way of better health and quality of life. The present work aims to socialize the results of the incorporation into the curriculum of the subject Food Chemistry and its corresponding reflective workshops, aimed at strengthening nutrition education. Theoretical, empirical and statistical-mathematical research methods were applied. The results achieved in the implementation of the proposal show its feasibility and relevance, also revealing a great acceptance in the students, given its contribution to the training of the professional.El objeto de la profesión del Licenciado en Educación Química Industrial, se concibe como un proceso conscientemente organizado de influencias educativas dirigido a la preparación integral del obrero competente, que tiene lugar en la integración institución educativa -entidad laboral -comunidad. Dentro de esa formación humanista se incluye la educación nutricional, como vía de una mejor salud y calidad de vida. El presente trabajo tiene como objetivo socializar los resultados de la incorporación en el currículo de la asignatura Química de los Alimentos y sus correspondientes talleres reflexivos, dirigidos a fortalecer la educación nutricional. Se aplicaron métodos de investigación teóricos, empíricos y estadístico-matemáticos. Los resultados alcanzados en la implementación de la propuesta muestran su factibilidad y pertinencia, revelándose también una gran aceptación en los estudiantes, dada su contribución a la formación del profesional

Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis

The reactivity of the 14F7 Mab, a highly specific IgG1 against N-glycolyl GM3 ganglioside (NeuGcGM3) in normal tissues, lymphomas, lymph node metastasis, and other metastatic sites was assessed by immunohistochemistry. In addition, the effect of chemical fixation on the 14F7 Mab staining using monolayers of P3X63Ag.653 cells was also evaluated. Moreover, the ability of 14F7 to bind NeuGcGM3 ganglioside inducing complement-independent cytotoxicity by a flow cytometry-based assay was measured. The 14F7 Mab was reactive in unfixed, 4% paraformaldehyde, 4% formaldehyde, and acetone fixed cells. Postfixation with acetone did not alter the localization of NeuGcGM3, while the staining with 14F7 Mab was significantly eliminated in both cells fixed and postfixed with methanol but only partially reduced with ethanol. The staining with 14F7 Mab was evidenced in the 89.2%, 89.4%, and 88.9% of lymphomas, lymph node metastasis, and other metastatic sites, respectively, but not in normal tissues. The treatment with 14F7 Mab affected both morphology and membrane integrity of P3X63Ag.653 cells. This cytotoxic activity was dose-dependent and ranged from 24.0 to 84.7% (10–1000 μg/mL) as compared to the negative control. Our data could support the possible use of NeuGcGM3 as target for both active and passive immunotherapy against malignancies expressing this molecule