Choisey – À l’Ormoy, Aux Champins, En Paroy

Liée à la réalisation du second tronçon de la liaison autoroutière Dijon-Bourg (A39), l’intervention archéologique conduite sur la commune de Choisey concernait trois secteurs distincts de la plaine alluviale du Doubs. Les décapages réalisés aux lieux-dits « En Paroy », « Aux Champins » et « À l’Ormoy », représentant une surface totale de 23 000 m2, permettaient la mise en évidence de trois grandes périodes d’occupation. Occupation mésolithique Détecté à l’occasion de la fouille des niveaux p..

Immersive Teleoperation of the Eye Gaze of Social Robots Assessing Gaze-Contingent Control of Vergence, Yaw and Pitch of Robotic Eyes

International audienceThis paper presents a new teleoperation system – called stereo gaze-contingent steering (SGCS) – able to seamlessly control the vergence, yaw and pitch of the eyes of a humanoid robot – here an iCub robot – from the actual gaze direction of a remote pilot. The video stream captured by the cameras embedded in the mobile eyes of the iCub are fed into an HTC Vive R Head-Mounted Display equipped with an SMI R binocular eye-tracker. The SGCS achieves the effective coupling between the eye-tracked gaze of the pilot and the robot's eye movements. SGCS both ensures a faithful reproduction of the pilot's eye movements – that is perquisite for the readability of the robot's gaze patterns by its interlocutor – and maintains the pilot's oculomotor visual clues – that avoids fatigue and sickness due to sensorimotor conflicts. We here assess the precision of this servo-control by asking several pilots to gaze towards known objects positioned in the remote environment. We demonstrate that we succeed in controlling vergence with similar precision as eyes' azimuth and elevation. This system opens the way for robot-mediated human interactions in the personal space, notably when objects in the shared working space are involved

Immersive Teleoperation of the Eye Gaze of Social Robots Assessing Gaze-Contingent Control of Vergence, Yaw and Pitch of Robotic Eyes

International audienceThis paper presents a new teleoperation system – called stereo gaze-contingent steering (SGCS) – able to seamlessly control the vergence, yaw and pitch of the eyes of a humanoid robot – here an iCub robot – from the actual gaze direction of a remote pilot. The video stream captured by the cameras embedded in the mobile eyes of the iCub are fed into an HTC Vive R Head-Mounted Display equipped with an SMI R binocular eye-tracker. The SGCS achieves the effective coupling between the eye-tracked gaze of the pilot and the robot's eye movements. SGCS both ensures a faithful reproduction of the pilot's eye movements – that is perquisite for the readability of the robot's gaze patterns by its interlocutor – and maintains the pilot's oculomotor visual clues – that avoids fatigue and sickness due to sensorimotor conflicts. We here assess the precision of this servo-control by asking several pilots to gaze towards known objects positioned in the remote environment. We demonstrate that we succeed in controlling vergence with similar precision as eyes' azimuth and elevation. This system opens the way for robot-mediated human interactions in the personal space, notably when objects in the shared working space are involved

Rapid and scalable synthesis of innovative unnatural α,β or γ-amino acids functionalized with tertiary amines on their side-chains.

We report a selective ruthenium catalyzed reduction of tertiary amides on the side chain of Fmoc-Gln-OtBu derivatives, leading to innovative unnatural α,β or γ-amino acids functionalized with tertiary amines. Rapid and scalable, this process allowed us to build a library of basic unnatural amino acids at the gram-scale and directly usable for liquid- or solid-phase peptide synthesis. The diversity of available tertiary amines allows us to modulate the physicochemical properties of the resulting amino acids, such as basicity or hydrophobicity.journal article2015 Jul 072015 06 01importe

Brief Communication External globus pallidus stimulation modulates brain connectivity in Huntington's disease

Positron emission tomography with O-15-labeled water was used to study at rest the neurophysiological effects of bilateral external globus pallidus (GPe) deep brain stimulation in patients with Huntington's disease (HD). Five patients were compared with a control group in the on and off states of the stimulator. External globus pallidus stimulation decreased neuronal activity and modulated cerebral connectivity within the basal ganglia-thalamocortical circuitry, the sensorimotor, and the default-mode networks. These data indicate that GPe stimulation modulates functional integration in HD patients in accordance with the basal ganglia-thalamocortical circuit model

Caractérisation moléculaire et structurale de la famille des protéines GASP

Les RCPG sont exprimés dans tous types de tissus et sont impliqués dans la régulation de nombreux processus biologiques et ont pour rôle de capter un vaste panel de stimuli extracellulaires qu ils transmettent à l intérieur de la cellule. Récemment, le laboratoire a identifié une nouvelle famille de dix protéines, les GASP, qui interagissent avec les RCPG et moduleraient leur trafic intracellulaire. Alors que GASP-1 est le membre de cette famille le mieux caractérisé et que son interaction avec de nombreux RCPG soit documentée, peu d informations sont disponibles sur les modalités d interaction de cette protéineavec les RCPG au niveau moléculaire. La première partie de ce projet de thèse a consisté à étudier les modalités d interaction entre les GASPs et les RCPG au niveau moléculaire. Nous avons ainsi pu montrer à l aide de techniques biochimiques et biophysiques, l importance d un motif répété et conservé de 15 acides aminés pour l interaction de GASP-1 avec divers RCPG. Par la suite, les résultats obtenus ont été exploités pour mettre en place un essai de criblage qui nous a permis d identifier des petites molécules capables de perturber l interaction entre GASP-1 et le récepteur beta-2 adrénergique. Enfin, l absence de données structurales sur les protéines de la famille GASP nous a ensuite poussé à la réalisation d études structurales de ces protéines à la fois par cristallographie et par RMN. Bien que les résultats obtenus ne nous aient pas encore permis d obtenir la structure de ces protéines, des expériences préliminaires de RMN ont permis deconfirmer l implication des acides aminés tryptophanes présents au sein des motifs GASP dans l interaction avec les RCPG.GPCRs represent one of the most diversified protein families in humans. They translate extracellular stimuli into intracellular signals to modulate a large panel of physiological processes making them unrivalled targets for development of new therapeutic agents. Recently, we identified the GASP family of proteins that interact with GPCRs and modulate the postendocyticfate of agonist activated receptors. GASP-1 is the well-characterized protein of this family and has been shown to be involved in the sorting of receptors that are quickly degraded following agonistpromoted internalization. Although GASP-1 was found to interact with numerous GPCR both in vitro and in vivo and that helix 8 of GPCRs is critically involved in this interaction, little is known about which region within GASP-1 is required for its interaction with GPCRs. In this work, we first present a detailed analysis of the molecular interaction between GASPs and GPCRs. By using biochemical and biophysical experiments we shown that the central domain of GASP-1 is critical for the interaction with GPCRs and that a conserved and repeated sequence of 15 amino acids plays a critical role in this interaction. In a second step, we developed an HTC assay allowing us to identify small molecules able to disrupt the interaction between GASP-1 and the beta-2 adrenergic receptor. Finally, preliminary NMR experiments have confirmed the importance of amino acid tryptophan for the interaction with GPCRs and a first crystallization trials were performed with a fragment of GASP -1.STRASBOURG-Bib.electronique 063 (674829902) / SudocSudocFranceF