Romantic Relationship Stages and Social Networking Sites: Uncertainty Reduction Strategies and Perceived Relational Norms on Facebook

Due to their pervasiveness and unique affordances, social media play a distinct role in the development of modern romantic relationships. This study examines how a social networking site is used for information seeking about a potential or current romantic partner. In a survey, Facebook users (N= 517) were presented with Facebook behaviors categorized as passive (e.g., reading a partner’s profile), active (e.g., ‘‘friending’’ a common third party), or interactive (e.g., commenting on the partner’s wall) uncertainty reduction strategies. Participants reported how normative they perceived these behaviors to be during four possible stages of relationship development (before meeting face-to-face, after meeting face-to-face, casual dating, and exclusive dating). Results indicated that as relationships progress, perceived norms for these behaviors change. Sex differences were also observed, as women perceived passive and interactive strategies as more normative than men during certain relationship stages

Media Portrayals of Romantic Relationship Maintenance: A Content Analysis of Relational Maintenance Behaviors on Prime-Time Television

Maintenance behaviors play an important role in sustaining relational states, especially within committed romantic relationships. Limited research, however, has considered media portrayals of these behaviors. From the framework of social cognitive theory, this content analysis examined relational maintenance behaviors portrayed by committed romantic couples (heterosexual, gay, and lesbian) on prime-time network television. The frequency of maintenance behaviors (positivity, understanding, self-disclosure, assurances, relationships talks, tasks, and networks) varied by type, valence, program genre, and couple type (married=civil union or cohabitating). Results indicated that comedies featured more frequent relationship maintenance behaviors than dramas. In addition, comedies exhibited more frequent negative maintenance behaviors than dramas. Differences also existed between couple regarding the types of maintenance behavior exhibited. No sex differences in maintenance behaviors were observed between male and female characters

Transposon Mutagenesis Identification of Polymicrobial Interaction Mechanisms Between Prokaryotic and Eukaryotic Microorganisms

Antibiotic resistance occurs when bacteria change in response to selective pressures induced by antibiotics, which has become a major concern worldwide and one of the biggest threats to global health. Antibiotic resistance can occur naturally, but the misuse and overuse of antibiotics is accelerating the process. One way to combat this process is to understand the different relationships between microbes, also known as polymicrobial interactions. Bacteria can interact with one another synergistically or antagonistically and understanding the mechanisms behind these interactions can lead to the discovery of new therapeutics or targets to fight and kill pathogenic microbes. The rarely pathogenic Gram-negative bacterium, Alcaligenes faecalis, has previously been shown in our lab as playing an important role in potentially fighting antibiotic and antifungal resistance due to its competitiveness during polymicrobial interaction. Our research has found that A. faecalis kills Bacillus cereus, Staphylococcus aureus, and Candida albicans. This is a unique characteristic as these targets encompass both prokaryotic (bacteria) and eukaryotic (fungi) microbes. These three species are known to cause numerous infections in humans and have increased cases of antibiotic and antifungal resistance. In the present study, we investigated the genetic elements A. faecalis utilizes to inhibit growth when interacting with B. cereus, S. aureus, and C. albicans. Transposon mutagenesis was performed to create a genetic library of A. faecalis loss-of-function mutants. These strains were then screened against all three microorganisms to determine which mutants no longer inhibited growth. The mutants that lacked zones-of-inhibition were sequenced to determine the gene that had been interrupted. BLAST analysis of these sequences identified a MFS transporter, a 2FE-2S iron sulfur binding protein, a mechanosensitive ion channel, and a glucose-6-phosphate isomerase as instrumental in this inhibitory mechanism. Results from this research study can be used to further study polymicrobial interactions and potentially discover new therapeutics to combat antimicrobial resistance

Finding freedom: Simone Weil, Hannah Arendt, and Herbert Marcuse on post-totalitarian politics

Simone Weil, Hannah Arendt and Herbert Marcuse are generally considered to be theorists quite distinct from one another. In this paper, I argue that there are notable similarities in their work, particularly with regards to their social and political critique of modern, technological society. Each of them emerged during World War II as social critics who attempted to trace the rise and success of totalitarian movements, while engaging with the most fundamental political issues of their times: collective action, the relationship of the individual to their society, and justice, among others. Using a selection of their most significant writings, I consider their views on freedom, rationality, and science and technology, along with their vision of politics, and emphasize their similar perspectives on these issues. I conclude with some deliberations about their core beliefs, and summarize the commitment of each to a post-totalitarian politics dedicated to the fulfillment of human potential

Within and without: identity and immigration in contemporary European politics

How do feelings of national identity shape the attitudes of Western Europeans toward immigrants and immigration policy? The three empirical studies presented in this dissertation explore this question. The first study develops a typology of national identities and maps them onto the commonly-used left-right dimension of political positionality. Then, by using each type in a multivariate regression analysis, I show that each the identity types have a differential impact on attitudes toward immigrant groups and immigration policy. Finally, I also show that the salience of each type of identity varies between countries in Western Europe. This analysis uses the large-N, cross-national survey data from thirteen Western European countries polled by the International Social Survey Program in 2003. The results provide insight into reason why debates about national identity and sovereignty are almost entirely absent in some Western European countries political discourse, while hotly contested in others. In the second study, the Netherlands is taken as a critical case study in the development of anti-immigrant attitudes. The data for this paper was drawn from two surveys, administered ten years apart: one administered in 1998 with the aid of the University of Utrecht, and my own survey in 2007. The shocking events that took place in the Netherlands during this decade were connected to the immigrant community either tangentially or directly. These unforeseeable events presented a natural experiment, making it possible to infer the impact of these events on attitudes toward immigrants. I show that negative attitudes toward Muslims increased significantly between the two surveys. The third paper adds an explicitly political dimension to the analysis of anti-immigrant sentiment. Using a multi-level model, I examine the relationship between strength of party cue and anti-immigrant sentiment. There is significant variation at the party level on this issue. The results show that political parties on the right have more coherent positions on immigration than parties on the left, and that the relationship between party cues and anti-immigrant sentiment is strong and significant: political cues do impact individual attitudes on this issue

Aβ Alters the DNA Methylation Status of Cell-fate Genes in an Alzheimer’s Disease Model

Alzheimer’s disease (AD) is characterized by neurofibrillary tangles and extracellular amyloid-β plaques (Aβ). Despite ongoing research, some ambiguity remains surrounding the role of Aβ in the pathogenesis of this neurodegenerative disease. While several studies have focused on the mutations associated with AD, our understanding of the epigenetic contributions to the disease remains less clear. To that end, we determined the changes in DNA methylation in differentiated human neurons with and without Aβ treatment. We isolated the DNA from neurons treated with Aβ or vehicle, and digested the two samples with either a methylation-sensitive (HpaII) or a methylation-insensitive (MspI) restriction endonuclease. The fragments were amplified and co- hybridized to a commercial promoter microarray. Data analysis revealed a subset of genomic loci that shows a significant change in DNA methylation following Aβ treatment in comparison to the control group. After mapping these loci to nearby genes, we discovered high enrichment for cell-fate genes that control apoptosis and neuronal differentiation. Finally, we incorporated three of those genes in a possible model suggesting the means by which Aβ contributes to the brain shrinkage and memory loss seen in AD

Serologic Evidence that Ascaris and Toxoplasma Infections Impact Inflammatory Responses to Helicobacter pylori in Colombians

Background: Helicobacter pylori-infected children from coastal Tumaco, Colombia, have more parasitism, and adults have lower gastric cancer risk compared with high-altitude Pasto/Tuquerres residents. Because helminth and Toxoplasma gondii infections alter helicobacter gastritis in rodent models, we determined whether seropositivity to Ascaris lumbricoides or T. gondii was associated with Th2-IgG1 or Th1-IgG2 responses to H. pylori. Methods: Sera (240) from the two populations were evaluated for A. lumbricoides and T. gondii seropositivity and results correlated with IgE and IgG isotype responses to H. pylori. Results: Most Tumaco children and adults were seropositive for A. lumbricoides (89%, 66%), T. gondii (59%, 98%), or both (45%, 66%). In contrast, seropositivity among Pasto/Tuquerres children was much lower (9%A. lumbricoides, 11%T. gondii, and 2% dual positive) but increased in adults (58%A. lumbricoides, 82%T. gondii, and 41% dual positive). A. lumbricoides seropositivity correlated with elevated IgE and anti-inflammatory Th2-IgG1 responses to H. pylori, while T. gondiigondii seropositivity was linked to elevated IgE, pro-inflammatory Th1-IgG2, IgG3, and IgG4 responses to H. pylori. Individuals with high T. gondii titers had reduced Th1-IgG2, IgG3, and IgG4 responses to H. pylori. Conclusions: Results support regional differences for childhood parasitism and indicate A. lumbricoides and T. gondii infections may impact inflammatory responses to H. pylori and partially explain differences in gastric cancer risk in Colombia.National Institutes of Health (U.S.) (Grant T32RR07036)National Institutes of Health (U.S.) (Grant PO1CA028842)National Institutes of Health (U.S.) (Grant P30ES02109

Ovarian Teratoid Carcinosarcoma Is an Aggressive Tumor of Probable Mullerian Derivation with a Carcinosarcomatous and Mixed Germ-Cell Morphology

Ovarian carcinosarcoma is also referred to as malignant mixed Mullerian tumor (MMMT). It is a rare neoplasm, and although it represents less than 5% of malignant ovarian tumors, it remains generally well-known among clinicians and pathologists. Rarer yet is ovarian teratoid carcinosarcoma, defined as carcinosarcoma with the added feature of immature neuroectodermal tissue, with or without elements of primitive germ cell tumor. To our knowledge, six ovarian teratoid carcinosarcomas have been reported in the literature [Matsuura et al. J Obstet Gynaecol Res. 2010 Aug; 36(4): 907–11]. These tumors resemble nasopharyngeal tumors of the same name. We report a 55-year-old woman seen at Orlando Health’s division of gynecological oncology whose pathology showed ovarian teratoid carcinosarcoma, and present what we believe to be a seventh report of this entity

Infant behavioral reactivity predicts change in amygdala volume 12 years later

The current study examined the link between temperamental reactivity in infancy and amygdala development in middle childhood. A sample (n = 291) of four-month-old infants was assessed for infant temperament, and two groups were identified: those exhibiting negative reactivity (n = 116) and those exhibiting positive reactivity (n = 106). At 10 and 12 years of age structural imaging was completed on a subset of these participants (n = 75). Results indicate that, between 10 and 12 years of age, left amygdala volume increased more slowly in those with negative compared to positive reactive temperament. These results provide novel evidence linking early temperament to distinct patterns of brain development over middle childhood