Ultrathin PTFE Coating for Hypodermic Needles Enabled by Mussel-Inspired PDA Adhesive Layer

Polytetrafluoroethylene (PTFE) is a synthetic fluoropolymer of tetrafluoroethylene, commonly known by its brand name Teflon®, which has many desirable properties such as a low coefficient of friction, high temperature resistance, anti-microbial properties, hydrophobicity, biocompatibility, and chemical resistance. One of the major challenges caused by the non-stick property of PTFE is its poor adhesion to substrates: PTFE coatings can be easily detached from surfaces under frictional forces and shear. The molecule polydopamine (PDA) was discovered as the key protein for adhesion between polytetrafluoroethylene and substrates of many materials, including steel, and thus has been used as a unique approach for coatings. In this undergraduate thesis research, PDA will be examined as an adhesive layer to coat ultrathin PTFE coatings onto stainless-steel hypodermic needles that are subjected to friction during penetration tests to determine effectiveness of reducing forces experienced during needle insertion. The friction and adhesion properties of the coated surface was studied by creating a fixture to hold a thin polyethylene terephthalate (PET) film, and using a Bruker UMT Microtribometer (Bruker UMT) to force needle penetration while measuring the corresponding forces felt by the needle. It was found that the tip of the needle penetrated the PET film at a force on average 44.4% lower than the uncoated needles. During testing, the maximum force felt by coated needles was 125 mN compared to 225 mN for the uncoated needles

Sex and Selfhood: What Feminist Philosophy Can Learn from Recent Ethnography in Ho Chi Minh City

This article explores the connection of class dynamics to the moral agency of sex workers and their clients. It revisits the analyses of several contemporary feminist theorists, placing these analyses in dialogue with a recent ethnographic study of the sex work industry in Ho Chi Minh City, Vietnam. In light of this comparative analysis, it is argued that accurate understanding and assessment of the moral agency of sex workers and their clients requires attunement to the complex and evolving class dynamics within which each is situated. Thus, while traditional frameworks for approaching this subject are useful, they are ultimately inadequate

Solving Optimal Control with Nonlinear Dynamics Using Sequential Convex Programming

Sequential convex programming (SCP) is a useful tool in obtaining real-time solutions to direct optimal control, but it is unable to adequately model nonlinear dynamics due to the linearization and discretization required. As nonlinear program solvers are not yet functioning in real-time, a tool is needed to bridge the gap between satisfying the nonlinear dynamics and completing execution fast enough to be useful. This paper presents a real-time control algorithm, sequential convex programming with nonlinear dynamics correction (SCPn), which ameliorates the performance of SCP under nonlinear dynamics. Simulations are presented to validate the efficacy of the method

No-vent fill pressurization tests using a cryogen simulant

The results are described of an experimental program which studied the performance of various no-vent fill techniques for tank-to-tank liquid transfer. The tests were performed using a cryogen simulant (Freon-114) and a test bed consisting of a multiple tank/plumbing network that enabled studies of a variety of different inlet flow and active mixing regimes. Several results and conclusions were drawn from the 26 transfer experiments comprising the program. Most notable was the significant improvement in fill performance (i.e., minimized fill time and maximized fill fraction) with increased agitation of the liquid surface. Another was the close correlation between measured condensation rates and those predicted by recent theories which express condensation as a function of turbulent eddy effects on the liquid surface. In most cases, test data exhibited strong agreement with an analytical model which accounts for tank heat transfer and thermodynamics in a 1 g environment

Multipurpose electric furnace system

A multipurpose electric furnace system of advanced design for space applications was developed and tested. This system is intended for use in the Apollo-Soyuz Test Program. It consists of the furnace, control package and a helium package for rapid cooldown

Food Pantries and Stigma: Users’ Concerns and Public Support

This study compares the perception of stigma measured as social distance between users (n = 40) and non-users (n = 202) of local food pantries in the Upper Midwest. Utilizing the concept of social distance to measure social disapproval and stigma with a new Food Pantry Stigma Scale, these nonprobability results indicated that users’ perception of stigma was significantly higher than the non-using public (Cohen’s d = 1.56). These findings suggest that public support for need-based use of local food pantries in the Upper Midwest is substantially higher than those facing food insecurity anticipate

Comparing synthetic refocusing to deconvolution for the extraction of neuronal calcium transients from light fields

Significance: Light-field microscopy (LFM) enables fast, light-efficient, volumetric imaging of neuronal activity with calcium indicators. Calcium transients differ in temporal signal-to-noise ratio (tSNR) and spatial confinement when extracted from volumes reconstructed by different algorithms. Aim: We evaluated the capabilities and limitations of two light-field reconstruction algorithms for calcium fluorescence imaging. Approach: We acquired light-field image series from neurons either bulk-labeled or filled intracellularly with the red-emitting calcium dye CaSiR-1 in acute mouse brain slices. We compared the tSNR and spatial onfinement of calcium signals extracted from volumes reconstructed with synthetic refocusing and Richardson-Lucy 3D deconvolution with and without total variation regularization. Results: Both synthetic refocusing and Richardson-Lucy deconvolution resolved calcium signals from single cells and neuronal dendrites in three dimensions. Increasing deconvolution iteration number improved spatial confinement but reduced tSNR compared to synthetic refocusing. Volumetric light-field imaging did not decrease calcium signal tSNR compared to interleaved, widefield image series acquired in matched planes. Conclusions: LFM enables high-volume rate, volumetric imaging of calcium transients in single cells (bulk-labeled), somata and dendrites (intracellular loaded). The trade-offs identified for tSNR, spatial confinement, and computational cost indicate which of synthetic refocusing or deconvolution can better realize the scientific requirements of future LFM calcium imaging applications

Adherence of internet-based cancer risk assessment tools to best practices in risk communication: Content analysis

BACKGROUND: Internet-based risk assessment tools offer a potential avenue for people to learn about their cancer risk and adopt risk-reducing behaviors. However, little is known about whether internet-based risk assessment tools adhere to scientific evidence for what constitutes good risk communication strategies. Furthermore, their quality may vary from a user experience perspective. OBJECTIVE: This study aims to understand the extent to which current best practices in risk communication have been applied to internet-based cancer risk assessment tools. METHODS: We conducted a search on August 6, 2019, to identify websites that provided personalized assessments of cancer risk or the likelihood of developing cancer. Each website (N=39) was coded according to standardized criteria and focused on 3 categories: general website characteristics, accessibility and credibility, and risk communication formats and strategies. RESULTS: Some best practices in risk communication were more frequently adhered to by websites. First, we found that undefined medical terminology was widespread, impeding comprehension for those with limited health literacy. For example, 90% (35/39) of websites included technical language that the general public may find difficult to understand, yet only 23% (9/39) indicated that medical professionals were their intended audience. Second, websites lacked sufficient information for users to determine the credibility of the risk assessment, making it difficult to judge the scientific validity of their risk. For instance, only 59% (23/39) of websites referenced the scientific model used to calculate the user\u27s cancer risk. Third, practices known to foster unbiased risk comprehension, such as adding qualitative labels to quantitative numbers, were used by only 15% (6/39) of websites. CONCLUSIONS: Limitations in risk communication strategies used by internet-based cancer risk assessment tools were common. By observing best practices, these tools could limit confusion and cultivate understanding to help people make informed decisions and motivate people to engage in risk-reducing behaviors

Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells

Cancer cells feature a resting membrane potential (Vm) that is depolarized compared to normal cells, and express active ionic conductances, which factor directly in their pathophysiological behavior. Despite similarities to ‘excitable’ tissues, relatively little is known about cancer cell Vm dynamics. Here high-throughput, cellular-resolution Vm imaging reveals that Vm fluctuates dynamically in several breast cancer cell lines compared to non-cancerous MCF-10A cells. We characterize Vm fluctuations of hundreds of human triple-negative breast cancer MDA-MB-231 cells. By quantifying their Dynamic Electrical Signatures (DESs) through an unsupervised machine-learning protocol, we identify four classes ranging from "noisy” to “blinking/waving“. The Vm of MDA-MB-231 cells exhibits spontaneous, transient hyperpolarizations inhibited by the voltage-gated sodium channel blocker tetrodotoxin, and by calcium-activated potassium channel inhibitors apamin and iberiotoxin. The Vm of MCF-10A cells is comparatively static, but fluctuations increase following treatment with transforming growth factor-β1, a canonical inducer of the epithelial-to-mesenchymal transition. These data suggest that the ability to generate Vm fluctuations may be a property of hybrid epithelial-mesenchymal cells or those originated from luminal progenitors