Effect of particle size and extrusion processing parameters on in vitro starch fractions, in vivo starch digestibility and glycemic index of field pea in dogs

A meta-analysis was performed to determine the effect of hydrothermal processing of peas on starch digestibility in monogastric species. From a total of 415 studies on hydrothermal processing of peas, nine studies were identified for inclusion in the meta-analysis. Effect sizes were standardized by converting them to Cohen’s d (CD). The combined nine studies showed a significant increase in pea starch digestibility by CD = 6.94 (95% CI; 4.50-9.37; P < 0.001) after hydrothermal treatment. A regression of processing temperature on the effect size showed a nearly significant quadratic response (CD = -0.009(temp)2 + 2.345(temp) – 146.103, r² = 0.303; P = 0.096). This suggests that the rate of pea starch digestion can be manipulated by controlling processing temperature. The hypothesis of this research was that processing parameters, namely particle size and extrusion, would alter pea starch in vitro degradability, and in vivo digestibility and glycemic response in laboratory beagles. A preliminary experiment found that, although not significant (P = 0.07), pea starch had a lower total tract apparent digestibility coefficient (TTADC) than rice starch (81% vs. 100% respectively) (n = 6). A second experiment found no significant effect of pea particle sizes 195, 309, and 427μm on glycemic index (GI) in laboratory beagles (n = 6). A third experiment was performed to determine the effect of extrusion processing on pea starch. The experiment used a completely randomized 2 x 2 x 2 x 2 factorial design with 2 levels of temperature (110 vs. 150°C), moisture (20 vs. 28%), particle size (288 vs. 407 μm) and cooling method (freezing vs. drying). Extrudates were analyzed for their rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS) contents. Particle size was the only significant effect; large particle size increased RS and decreased RDS (P < 0.05). There was also significant negative correlation between particle size and RDS and SDS fractions (P < 0.05) and a trend toward particle size being positively correlated with RS content (P = 0.059). Subsequently, four of the 16 extruded treatments were selected for the measurement of GI in beagles (n = 6): 3) 150°C, 288 μm, 20% H20, dried; 7) 110°C, 288 μm, 20% H20, dried; 10) 150°C, 407 μm, 28% H20, frozen; 14) 110°C, 407 μm, 28% H20, frozen. There was no relationship between GI and particle size, but GI was negatively and RDS was positively correlated with temperature (P < 0.05). These results suggest that in vitro starch fractions are not good predictors of GI in dogs. However, the rate of pea starch digestion may be manipulated by controlling processing temperature. Further studies are needed to determine the effect of multiple temperatures on the GI of starch

Dietary Fiber and Intestinal Health of Monogastric Animals

Animal performance, feed efficiency, and overall health are heavily dependent on gut health. Changes in animal production systems and feed regulations away from the use of antibiotic growth promoters (AGP) have necessitated the identification of strategies to optimize gut health in novel and effective ways. Among alternatives to AGP, the inclusion of dietary fibers (DF) in monogastric diets has been attempted with some success. Alternative feedstuffs and coproducts are typically rich in fiber and can be used in the diets to reduce feed costs and optimize gut health. DF are naturally occurring compounds with a diverse composition and are present in all plant-based feedstuffs. DF stimulate the growth of health-promoting gut bacteria, are fermented in the distal small intestine and large intestine to short-chain fatty acids and have beneficial effects on the immune system. Maternal DF supplementation is one novel strategy suggested to have a beneficial programming effect on the microbial and immune development of their offspring. One mechanism by which DF improves gut health is through maintenance of an anaerobic intestinal environment that subsequently prevents facultative anaerobic pathogens from flourishing. Studies with pigs and poultry have shown that fermentation characteristics and their beneficial effects on gut health vary widely based on type, form, and the physico-chemical properties of the DF. Therefore, it is important to have information on the different types of DF and their role in optimizing gut health. This review will provide information and updates on different types of DF used in monogastric nutrition and its contribution to gut health including microbiology, fermentation characteristics, and innate and adaptive immune responses

Dietary Pea Fiber Supplementation Improves Glycemia and Induces Changes in the Composition of Gut Microbiota, Serum Short Chain Fatty Acid Profile and Expression of Mucins in Glucose Intolerant Rats

Several studies have demonstrated the beneficial impact of dried peas and their components on glucose tolerance; however, the role of gut microbiota as a potential mediator is not fully examined. In this study, we investigated the effect of dietary supplementation with raw and cooked pea seed coats (PSC) on glucose tolerance, microbial composition of the gut, select markers of intestinal barrier function, and short chain fatty acid profile in glucose intolerant rats. Male Sprague Dawley rats were fed high fat diet (HFD) for six weeks to induce glucose intolerance, followed by four weeks of feeding PSC-supplemented diets. Cooked PSC improved glucose tolerance by approximately 30% (p &lt; 0.05), and raw and cooked PSC diets reduced insulin response by 53% and 56% respectively (p &lt; 0.05 and p &lt; 0.01), compared to HFD (containing cellulose as the source of dietary fiber). 16S rRNA gene sequencing on fecal samples showed a significant shift in the overall microbial composition of PSC groups when compared to HFD and low fat diet (LFD) controls. At the family level, PSC increased the abundance of Lachnospiraceae and Prevotellaceae (p &lt; 0.001), and decreased Porphyromonadaceae (p &lt; 0.01) compared with HFD. This was accompanied by increased mRNA expression of mucin genes Muc1, Muc2, and Muc4 in ileal epithelium (p &lt; 0.05). Serum levels of acetate and propionate increased with raw PSC diet (p &lt; 0.01). These results indicate that supplementation of HFD with PSC fractions can improve glycemia and may have a protective role against HFD-induced alterations in gut microbiota and mucus layer

Host Immune Selection of Rumen Bacteria through Salivary Secretory IgA

The rumen microbiome is integral to efficient production in cattle and shows strong host specificity, yet little is known about what host factors shape rumen microbial composition. Secretory immunoglobulin A (SIgA) is produced in large amounts in the saliva, can coat both commensal and pathogenic microbes within the gut, and presents a plausible mechanism of host specificity. However, the role salivary SIgA plays in commensal bacteria selection in ruminants remains elusive. The main objectives of this study were to develop an immuno-affinity benchtop method to isolate SIgA-tagged microbiota and to determine if salivary SIgA preferentially binds selected bacteria. We hypothesized that SIgA-tagged bacteria would differ from total bacteria, thus supporting a potential host-derived mechanism in commensal bacterial selection. Whole rumen (n = 9) and oral secretion samples (n = 10) were incubated with magnetic beads conjugated with anti-secretory IgA antibodies to enrich SIgA-tagged microbiota. Microbial DNA from the oral secretion, whole rumen, SIgA-tagged oral secretion, and SIgA-tagged rumen was isolated for amplicon sequencing of V1–V3 region of 16S rDNA genes. Whole rumen and oral secretion had distinctive (P &lt; 0.05) bacterial compositions indicated by the non-parametric multidimensional scaling plot using Euclidean distance metrics. The SIgA-tagged microbiota from rumen and oral secretion had similar abundance of Bacteroidetes, Actinobacteria, Fibrobacter, candidate phyla TM7, and Tenericutes and are clustered tightly. Composition of SIgA-tagged oral secretion microbiota was more similar to whole rumen microbiota than whole oral secretion due to enrichment of rumen bacteria (Lachnospiraceae) and depletion of oral taxa (Streptococcus, Rothia, Neisseriaceae, and Lactobacillales). In conclusion, SIgA-tagged oral secretion microbiota had an increased resemblance to whole rumen microbiota, suggesting salivary SIgA-coating may be one host-derived mechanism impacting commensal colonization. Further studies, to explore the variations in antibody affinity between different animals as a driver of microbial composition are warranted

Putting the microbiota to work: Epigenetic effects of early life antibiotic treatment are associated with immune-related pathways and reduced epithelial necrosis following Salmonella Typhimurium challenge in vitro.

Salmonella enterica serovar Typhimurium is an animal welfare and public health concern due to its ability to parasite livestock and potentially contaminate pork products. To reduce Salmonella shedding and the risk of pork contamination, antibiotic therapy is used and can contribute to antimicrobial resistance. Here we hypothesized that immune system education by the microbiota can play a role in intestinal resilience to infection. We used amoxicillin (15mg/Kg) to modulate the intestinal microbiome of 10 piglets, paired with same age pigs that received a placebo (n = 10) from 0 to 14 days of age. Animals were euthanized at 4-weeks old. Each pig donated colon sections for ex vivo culture (n = 20 explants/pig). Explants were inoculated with S. Typhimurium, PBS or LPS (n = 6 explants/pig/group, plus technical controls). The gut bacteriome was characterized by sequencing of the 16S rRNA at 7, 21 days of age, and upon in vitro culture. Explants response to infection was profiled through high-throughput mRNA sequencing. In vivo antibiotic treatment led to β-diversity differences between groups at all times (P<0.05), while α-diversity did not differ between amoxicillin and placebo groups on day 21 and at euthanasia (P<0.03 on day 7). Explant microbiomes were not different from in vivo. In vitro challenge with S. Typhimurium led to lower necrosis scores in explants from amoxicillin-treated pigs, when compared to explants placebo-treated pigs (P<0.05). This was coupled with the activation of immune-related pathways in explants from amoxicillin-treated pigs (IL-2 production, NO production, BCR activation), when compared to placebo-treated pigs. In addition, several DNA repair and intestinal wound healing pathways were also only activated in explants from amoxicillin-treated pigs. Taken together, these findings suggest that immune education by the amoxicillin-disturbed microbiota may have contributed to mitigate intestinal lesions following pathogen exposure

Outcomes of a low birth weight phenotype on piglet gut microbial composition and intestinal transcriptomic profile

Decades of selection for increased litter size has caused a proportion of sows to consistently produce low birth weight (LBW) litters resulting in economic loss for producers due to reduced piglet survivability and growth. We hypothesized that piglets from LBW litters would have altered gut microbial composition, intestinal architecture, and intestinal transcriptomic profiles compared with piglets from high birth weight (HBW) litters. Sows were designated LBW (n = 45) or HBW (n = 46) based on litter birth weights of three successive parities. LBW piglets were 22% lighter (P  0.05) in weight at weaning compared with HBW piglets. LBW piglets had reduced (P < 0.05) fecal microbial diversity with a 114% increase in fecal Enterobacteriaceae (P < 0.05), as well as reduced (P < 0.05) abundance of cecal Roseburia and Faecalibacterium, fiber-degrading butyrate producers. Several genes associated with metabolic (PER2, CES1, KLHL38, and HK2) and immune pathways (IL-1B, IRF8, and TNIP3) were differentially expressed, suggesting altered metabolic and immune function in LBW piglets. In conclusion, LBW piglets had potentially unfavorable shifts in microbial structure in comparison to HBW piglets accompanied with alterations in metabolic and immune gene expression. Results indicate some biological consequences linking LBW phenotype to changes in production efficiency later in life.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

High Vaccenic Acid Content in Beef Fat Attenuates High Fat and High Carbohydrate Western Diet Induced Changes in Lipid Metabolism and Gut Microbiota in Pigs

High-fat diets (HFD) have been shown to induce substantial shifts in intestinal microbial community composition and activity which are associated with adverse metabolic outcomes. Furthermore, changes in microbial composition are affected by fatty acid composition; saturated, monounsaturated (MUFA), and industrial trans fats (iTFA) adversely affect microbial diversity while polyunsaturated fats (PUFA) have been shown to have neutral effects. The effects of naturally occurring trans fats on gut microbial composition are unknown. Vaccenic acid (VA) is the most abundant naturally occurring trans fat (abundant in meat and dairy), can be elevated by altering a cow’s diet, and has been shown to have hypolipidemic effects. The aim of this study was to determine how variations of VA content in beef fat affect gut microbial composition, insulin resistance, and lipid metabolism in pigs. Low birth weight (LBW) and control pigs were fed a control or high-fat, high-carbohydrate (HFHC) diet supplemented with beef fat containing either high or low VA levels for 7 weeks. An adapted modified oral glucose tolerance test and fat challenge test were performed at 9 weeks of age following implantation of jugular catheters. Impacts on microbial composition were assessed using 16S rRNA gene amplicon sequencing. The HFHC diet containing beef fat rich in VA had a mild insulin sensitizing effect (p p p < 0.05), and showed protection from HFHC-induced changes to gut microbial composition in LBW pigs as compared to HFHC diet containing standard beef fat. This is the first study to show effects of natural trans fats on gut dysbiosis; further studies are needed to elucidate mechanisms