29,891 research outputs found
COMMODITY POLICY, PRICE INCENTIVES, AND THE GROWTH IN PER-ACRE YIELDS
We estimate the influence of policy-induced price changes and of technology supply on North Carolina flue-cured tobacco yields. The decline in land rent and effective output price that accompanied a 1965 policy change from acreage allotments to poundage quotas caused a 12 percent decrease in yields. Farmer yields were more responsive to yield-increasing technologies under acreage allotments than under poundage quotas. Annual yield growth was 0.5 percent under poundage quotas and 4.32 percent under acreage allotments. The growth rate decline is attributable to changes in relative prices and to a slowdown in the supply of available technologies.Commodity policy, Endogenous yield growth, Flue-cured tobacco, Technical change, Agricultural and Food Policy, Demand and Price Analysis, Research and Development/Tech Change/Emerging Technologies,
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Congenital imperforate hymen with hydrocolpos and hydronephrosis associated with severe hydramnios and increase of maternal ovarian steroidogenic enzymes
This is a clinical research paperStudy Objective: To study clinical features of patient presented with severe hydramnios, associated with hydronephrosis, that was antenatally diagnosed and has
been successfully treated immediately after birth. At a molecular level, we investigated the gene expression of key steroidogenic enzymes from the maternal ovary.
Design: Ultrasound scan,MRI, semi-quantitativeRT-PCR
Setting: The patient was admitted to the University Hospital,
University of Crete, Medical School, Greece, where
all clinical data has been obtained. Gene expression studies
took place at Biosciences, Brunel University, UK.
Results: Semi-quantitative RT-PCR analyses revealed
that there is upregulation of key steroidogenic genes in
the maternal ovary, including steroidogenic acute regulatory
protein, and the cytochrome P450 heme-containing
proteins CYP11A, CYP17 and CYP19. From a clinical perspective,
the prenatal ultrasound scan and MRI findings
showed a multicystic pelvic mass, bilateral hydronephrosis
and prior to delivery severe polyhydramnios.
Conclusion: This clinical case is the only one that we
have found in the current literature where congenital imperforate
hymen accompanied with hematocolpos is associated
with renal obstruction in combination with polyhydramnios
and increase in maternal steroidogenic enzymes
Expression of mTOR and downstream signalling components in the JEG-3 and BeWo human placental choriocarcinoma cell lines
This article has been made available through the Brunel Open Access Publishing Fund.Emerging data suggest that nutritional status and body weight are related to reproductive function, and nutrient imbalances during pregnancy lead to changes in the expression of fetal genes. Recent studies show that the mTOR acts as a placental growth signalling sensor and its expression is down-regulated in intrauterine growth restriction. To date, very little is known about the expression of this signalling pathway in choriocarcinoma, one of the most lethal germ cell cancers. In this study, cultures of fusigenic (BeWo) and non-fusigenic (JEG-3) human choriocarcinoma cell lines were used to investigate the expression of mTOR and its downstream signalling components. The effects of an inducer of syncytialisation (forskolin) on mTOR, eIF4E binding proteins (4EBPs) and ribosomal protein S6 kinases (S6Ks) in BeWo cells were also assessed. RT-PCR studies revealed that mTOR, 4EBP and S6Ks are expressed at mRNA level in both JEG-3 and BeWo cells. Semi-quantitative RT-PCR analysis revealed that in early stages of syncytialisation (50 µM forskolin for 48 h), the expression of mTOR and 4EBP was down-regulated when compared to unstimulated cells. In fully syncytialised cells (50 µM forskolin for 72 h) the expression of both genes was similar to basal levels. Interestingly, the phosphorylation (Ser371, Thr389) status of p70S6K remained unaltered upon forskolin treatment. These data validate BeWo cells as an experimental model to study the effects of forskolin-induced syncytialisation on mTOR signalling.This article is available through the Brunel Open Access Publishing Fun
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Expression of membrane and nuclear progesterone receptors in two human placental choriocarcinoma cell lines (JEG-3 and BeWo): Effects of syncytialization
This article has been made available through the Brunel Open Access Publishing Fund and is available from the specified link -
Copyright @ 2011 Spandidos Publications Ltd.A vital function of the human placenta is to produce steroid hormones such as progesterone, which are essential for the maintenance of pregnancy and the onset of parturition. Although choriocarcinoma cell lines are valuable placental models for investigations of steroid hormone actions, little is known about the expression of progesterone receptors (PRs) in these cell lines. Therefore, in this study, the expression of membrane and nuclear PRs was investigated in cultures of fusigenic (BeWo) and non-fusigenic (JEG-3) human choriocarcinoma cell lines. In addition, the effects of an inducer of syncytialization (forskolin) on the PR expression in BeWo cells were assessed. Quantitative RT-PCR revealed that in fully syncytialized BeWo cells (treated with 50 mu M forskolin for 72 h) there was a significant down-regulation of mPR alpha and up-regulation of mPR beta and of the progesterone membrane component-1 (PGRMC1) when compared with non-syncytialized BeWo cells. Expression of all the mPR and PGRMC1 mRNAs was significantly lower in JEG-3 cells compared to non-syncytialized BeWo cells. Interestingly, expression of PR-B was unaltered between the two BeWo states but was significantly higher in JEG-3 cells. Immunofluorescence analysis revealed that mPR proteins are differentially expressed in these choriocarcinoma cell lines as well as in the human placenta. The data demonstrate that human choriocarcinoma cell lines have a complex system of progesterone signalling involving multiple classes of PRs. The finding that syncytialization is accompanied by changes in the expression of these receptors may suggest that this process influences progesterone signalling
Applied Remote Sensing Program (ARSP)
Descriptions of projects engaged by the Applied Remote Sensors Program in the state of Arizona are contained in an annual report for the fiscal year 1976-1977. Remote sensing techniques included thermal infrared imagery in analog and digital form and conversion of data into thermograms. Delineation of geologic areas, surveys of vegetation and inventory of resources were also presented
The Y-Band at 1.035 um: Photometric Calibration and the Dwarf Stellar/Sub-Stellar Color Sequence
We define and characterize a photometric bandpass (called "Y") that is
centered at 1.035 um, in between the traditionally classified ``optical'' and
``infrared'' spectral regimes. We present Y magnitudes and Y-H and Y-K colors
for a sample consisting mostly of photometric and spectral standards, spanning
the spectral type range sdO to T5V. Deep molecular absorption features in the
near-infrared spectra of extremely cool objects are such that the Y-H and Y-K
colors grow rapidly with advancing spectral type especially from late M through
mid L, substantially more rapidly than J-H or H-K which span a smaller total
dynamic range. Consistent with other near-infrared colors, however, Y-H and Y-K
colors turn blueward in the L6-L8 temperature range with later T-type objects
having colors similar to those of warmer M and L stars. Use of the Y-band
filter is nonetheless promising for easy identification of low-mass stars and
brown dwarfs, especially at young ages. The slope of the interstellar reddening
vector within this filter is A_Y = 0.38 x A_V. Reddening moves stars nearly
along the YHK dwarf color sequence making it more difficult to distinguish
unambiguously very low mass candidate brown dwarf objects from higher mass
stars seen, e.g. through the galactic plane or towards star-forming regions.
Other diagrams involving the Y-band may be somewhat more discriminating.Comment: accepted at PAS
Modelling energy consumption in supermarkets to reduce energy use and greenhouse gas emissions using EnergyPlus
New refrigeration system configurations and other innovating technologies in retail supermarkets need to be considered to reduce energy use and greenhouse gas emissions. In supermarkets, there is a strong interaction between the refrigerated display cases, supermarket structure, internal machinery, customers, and the store’s HVAC system. The impact of these interactions on the energy and carbon emissions of a medium sized supermarket in Paris was modelled using EnergyPlus™. The results were calibrated against a typical UK store and validated against the Paris store. The effects of applying the technologies identified to have the greatest potential to reduce carbon emissions (changing the refrigerant to R744, switching from gas to electrical heating and adding doors to chilled cabinets) were modelled. The impact of climate change on ambient temperature and the impact of changes to the grid conversion factor were predicted for the store in Paris from 2020 to 2050
Systematic derivation of a surface polarization model for planar perovskite solar cells
Increasing evidence suggests that the presence of mobile ions in perovskite
solar cells can cause a current-voltage curve hysteresis. Steady state and
transient current-voltage characteristics of a planar metal halide
CHNHPbI perovskite solar cell are analysed with a drift-diffusion
model that accounts for both charge transport and ion vacancy motion. The high
ion vacancy density within the perovskite layer gives rise to narrow Debye
layers (typical width 2nm), adjacent to the interfaces with the transport
layers, over which large drops in the electric potential occur and in which
significant charge is stored. Large disparities between (I) the width of the
Debye layers and that of the perovskite layer (600nm) and (II) the ion
vacancy density and the charge carrier densities motivate an asymptotic
approach to solving the model, while the stiffness of the equations renders
standard solution methods unreliable. We derive a simplified surface
polarisation model in which the slow ion dynamic are replaced by interfacial
(nonlinear) capacitances at the perovskite interfaces. Favourable comparison is
made between the results of the asymptotic approach and numerical solutions for
a realistic cell over a wide range of operating conditions of practical
interest.Comment: 32 pages, 7 figure
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Internalisation of membrane progesterone receptor-α after treatment with progesterone: Potential involvement of a clathrin-dependent pathway
This article has been made available through the Brunel Open Access Publishing Fund.Internalisation and recycling of seven trans-membrane domain receptors is a critical regulatory event for their signalling. The mechanism(s) by which membrane progesterone receptor-α (mPRα) number is regulated on the cell surface is unclear. In this study, we investigated the cellular distribution of mPRα and mechanisms of mPRα trafficking using a cell line derived from a primary culture of human myometrial cells (M11) as an experimental model. RT-PCR and immunofluorescent analysis demonstrated expression of mPRα in M11 cells with mPRα primarily distributed on the cell surface under basal conditions. For the first time, plasma membrane localisation of mPRα was confirmed using immuno-gold transmission electron microscopy. Stimulation of M11 cells with progesterone (P4, 100 nM) resulted in internalisation of mPRα from the plasma membrane to the cytoplasm (10 min) and subsequent partial translocation back to the cell surface (20 min). We investigated potential endocytotic pathways involved in trafficking of mPRα after its internalisation. Partial co-localisation of clathrin with mPRα was obvious after 10 min of P4 treatment. Of note, chlorpromazine (inhibitor of clathrin-mediated pathway) inhibited the endocytosis of mPRα, whereas treatment with nystatin (inhibitor of caveolae-mediated pathway) did not affect internalisation. Collectively, these data suggest that mPRα is expressed on the cell surface of M11 cells and undergoes endocytosis after P4 stimulation primarily via a clathrin-mediated pathway.This article is available through the Brunel Open Access Publishing Fun
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Differential expression of mTOR signalling components in drug resistance in ovarian cancer
This article has been made available through the Brunel Open Access Publishing Fund and is available from the specified link - Copyright @ 2010 The International Institute of Anticancer Research.Background/Aim: A limitation to successful cancer chemotherapy treatments is the acquisition of drug resistance. In advanced-stage ovarian cancer, the mammalian target of rapamycin (mTOR) pathway is upregulated, and inhibition of this pathway increases chemosensitivity in ovarian carcinoma cell lines. In this study, the expression of DEPTOR, mTOR, RICTOR, RAPTOR and S6 kinases were investigated in SKOV-3 and PEO1 parental and the paclitaxel-resistant (TaxR) SKOV-3TaxR and PEO1TaxR cell lines. Materials and Methods: RT-PCR, immunofluorescent analysis and Western blotting were carried out. Results: Quantitative RT-PCR revealed significant up-regulation of DEPTOR in both paclitaxel-resistant cell lines. SKOV-3TaxR exhibited down-regulation of RICTOR, RAPTOR and mTOR, whereas PEO1-TaxR showed down-regulation of RAPTOR and up-regulation of RICTOR and mTOR. Semi-quantitative RT-PCR analysis revealed marked changes in the expression of p70S6K splice variants mRNA in PEO1TaxR. Moreover, the phosphorylation status of p70S6K at Ser371 appears to be cell-type specific. Conclusion: We hypothesize that mTOR signalling may play a role in mediating paclitaxel resistance in ovarian cancer
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