Conformations of the Single-Stranded DNA of Bacteriophage M13

At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100°C in 1.0 M NaCl. The ability to assume either conformation appears to be a function of the nucleic acid alone. Whether or not these different conformations are of biological significance is still unknown

Panel III: Cybersecurity for the Practitioner: Client Security, Discovery, and Ethical Considerations

* 1.5 MCLE- Ethics credits Moderator: Professor Ronald Rotunda, Chapman University Dale E. Fowler School of Law Eli Wald – University of Denver, Sturm College of Law Drew Simshaw – Attorney & Teaching Fellow, Institute for Public Representation, Georgetown Law Tanya L. Forsheit – Partner, Baker & Hostetler LLP, Los Angeles Scott B. Garner – Advisor and former Chair, Committee on Professional Responsibility and Conduct, State Bar of California; and Attorney, Umberg Zipser LL