Excited State Specific Multi-Slater Jastrow Wave Functions

We combine recent advances in excited state variational principles, fast multi-Slater Jastrow methods, and selective configuration interaction to create multi-Slater Jastrow wave function approximations that are optimized for individual excited states. In addition to the Jastrow variables and linear expansion coefficients, this optimization includes state-specific orbital relaxations in order to avoid the compromises necessary in state-averaged approaches. We demonstrate that, when combined with variance matching to help balance the quality of the approximation across different states, this approach delivers accurate excitation energies even when using very modest multi-Slater expansions. Intriguingly, this accuracy is maintained even when studying a difficult chlorine-anion-to-$\pi^{*}$ charge transfer in which traditional state-averaged multi-reference methods must contend with different states that require drastically different orbital relaxations.Comment: 16 pages, 6 figures, 2 table

Adenoviral delivery of angiotensin-(1-7) or angiotensin-(1-9) inhibits cardiomyocyte hypertrophy via the mas or angiotensin Type 2 receptor

The counter-regulatory axis of the renin angiotensin system peptide angiotensin-(1-7) [Ang-(1-7)] has been identified as a potential therapeutic target in cardiac remodelling, acting via the mas receptor. Furthermore, we recently reported that an alternative peptide, Ang-(1-9) also counteracts cardiac remodelling via the angiotensin type 2 receptor (AT(2)R). Here, we have engineered adenoviral vectors expressing fusion proteins which release Ang-(1-7) [RAdAng-(1-7)] or Ang-(1-9) [RAdAng-(1-9)] and compared their effects on cardiomyocyte hypertrophy in rat H9c2 cardiomyocytes or primary adult rabbit cardiomyocytes, stimulated with angiotensin II, isoproterenol or arg-vasopressin. RAdAng-(1-7) and RAdAng-(1-9) efficiently transduced cardiomyocytes, expressed fusion proteins and secreted peptides, as demonstrated by western immunoblotting and conditioned media assays. Furthermore, secreted Ang-(1-7) and Ang-(1-9) inhibited cardiomyocyte hypertrophy (Control = 168.7±8.4 µm; AngII = 232.1±10.7 µm; AngII+RAdAng-(1-7) = 186±9.1 µm, RAdAng-(1-9) = 180.5±9 µm; P<0.05) and these effects were selectively reversed by inhibitors of their cognate receptors, the mas antagonist A779 for RAdAng-(1-7) and the AT(2)R antagonist PD123,319 for RAdAng-(1-9). Thus gene transfer of Ang-(1-7) and Ang-(1-9) produces receptor-specific effects equivalent to those observed with addition of exogenous peptides. These data highlight that Ang-(1-7) and Ang-(1-9) can be expressed via gene transfer and inhibit cardiomyocyte hypertrophy via their respective receptors. This supports applications for this approach for sustained peptide delivery to study molecular effects and potential gene therapeutic actions

Instantaneous tunneling of relativistic massive spin-0 particles

The tunneling time problem earlier studied in Phys. Rev. Lett. 108, 170402 (2012) https://link.aps.org/doi/10.1103/PhysRevLett.108.170402 using a non-relativistic time-of-arrival (TOA) operator predicted that tunneling time is instantaneous implying that the wavepacket becomes superluminal below the barrier. The non-relativistic treatment raises the question whether the superluminal behavior is a mere non-relativistic phenomenon or an an inherent quantum effect in all energy scales. Here we extend the analysis by constructing a relativistic TOA-operator for spin-0 particles across a square potential barrier by quantizing the corresponding classical quantity, and imposing that the barrier height $V_o$ is less than the rest mass energy. We show that only the above barrier energy components of the incident wavepacket's momentum distribution contribute to the barrier traversal time while the below barrier components are transmitted instantaneously

Efecto oxitócico in vitro del extracto acuoso de las hojas de passiflora peduncularis (poro poro) en utero estrogenizado de cavia porcellus. laboratorio de farmacología de la universidad privada Antenor Orrego año 2014

Con el objetivo de determinar el efecto oxitócico del extracto acuoso de las hojas de la Passiflora peduncularis (poro poro) en el útero estrogenizado de Cavia porcellus; la muestra estuvo constituida por 12 cuyes: 24 cuernos. Se realizó un estudio farmacológico de estímulo creciente mediante el método de Perry W.L.M (The Rat Uterus Preparation) con musculo uterino estrogenizado, en relación con la acción de la Oxitócina. Se determinó la Dosis efectiva 50 (DE50) de la extracto acuoso de las hojas de Passiflora peduncularis en útero estrogenizado de cavia porcellus in vitro, la cual fue de 0.8 ml. Para investigar la actividad farmacológica en su aspecto útero contráctil en cavia porcellus in vitro, se utiliza la Dosis efectiva 50 (DE50) de la extracto acuoso de las hojas de Passiflora peduncularis (grupo problema). De esta investigación se concluyó que de la extracto acuoso de las hojas de Passiflora peduncularis en su eficacia oxitócica in vitro en útero estrogenizado de Cavia porcellus tiene menos amplitud y es menos activo en duración, durante la contracción en relación a la oxitocina, sin embargo este presenta mayor frecuencia.In order to determine the effect of aqueous extract oxytocic leaves peduncularis Passiflora (pore pore) in the uterus estrogenizado Cavys; The sample consisted of 12 guinea pigs: 24 horns. A pharmacological study was performed by increased stimulation method WLM Perry (The Rat Uterus Preparation) with estrogenizado uterine muscle in relation to the action of oxytocin. The effective dose 50 (ED50) of the aqueous extract of leaves in estrogenizado Passiflora uterus peduncularis Cavia porcellus determined in vitro, which was 0.8 ml. To investigate the pharmacological activity in appearance Cavia porcellus contractile uterus in vitro, the effective dose 50 (ED50) infusion Passiflora peduncularis leaves (problem group) is used. From this investigation it was concluded that the infusion of the leaves of Passiflora peduncularis in its oxytocin in vitro efficacy in estrogenizado uterus Cavia porcellus has less amplitude and is less active in duration, during contraction related to oxytocin, however this pre111sents more frequently.Tesi

The double-edged sword of learning from disasters: Mortality in the Tohoku tsunami

Learning from natural disasters is predominantly regarded as beneficial: Individuals and governments learn to cope and thereby reduce damage and loss of life in future disasters. We argue against this standard narrative and point to two principal ways in which learning from past disasters can have detrimental consequences: First, investment in protective infrastructures may not only stimulate settlement in hazard-prone areas but also foster a false impression of security, which can prevent individuals from fleeing to safe places when hazard strikes. Second, if disaster events in the past did not have catastrophic consequences, affected individuals do not take future events sufficiently seriously. As a consequence, learning from disasters is a double-edged sword that can prevent large scale damage and human loss most of the time but results in the worst case scenario when a disaster occurs at an unexpected scale and public preparedness measures fail. We demonstrate the devastating impact of misplaced trust in public preparedness measures and misleading lessons drawn from past experience for the case of the 2011 Tohoku tsunami. Our paper contributes to the literatures on ‘negative learning’ and ‘hazard maladaptation’ by demonstrating that a lack of past experience with tsunami mortality in a municipality substantively increases mortality in the Tohoku tsunami