A Framework for Building Technological Learning: Evidence from the New Zealand Dairy Industry

One aspect of the process of technology adoption is technological learning (TL), the way farmers gather information and turn it into knowledge. In a study of the New Zealand dairy industry, researchers examined the factors that affect TL. Findings suggest that the speed with which farmers engage in TL is influenced by the efficiency of the innovation system, the maturity of the farm system, and the individual characteristics of the farmer. The article presents a model demonstrating how these three sets of factors may affect TL that can be used by Extension agents to help them develop a strategy for engaging farmers in TL

New pleiotropic effects of eliminating a rare tRNA from Streptomyces coelicolor, revealed by combined proteomic and transcriptomic analysis of liquid cultures

Background: In Streptomyces coelicolor, bldA encodes the only tRNA for a rare leucine codon, UUA. This tRNA is unnecessary for growth, but is required for some aspects of secondary metabolism and morphological development. We describe a transcriptomic and proteomic analysis of the effects of deleting bldA on cellular processes during submerged culture: conditions relevant to the industrial production of antibiotics. Results: At the end of rapid growth, a co-ordinated transient up-regulation of about 100 genes, including many for ribosomal proteins, was seen in the parent strain but not the ΔbldA mutant. Increased basal levels of the signal molecule ppGpp in the mutant strain may be responsible for this difference. Transcripts or proteins from a further 147 genes classified as bldA-influenced were mostly expressed late in culture in the wild-type, though others were significantly transcribed during exponential growth. Some were involved in the biosynthesis of seven secondary metabolites; and some have probable roles in reorganising metabolism after rapid growth. Many of the 147 genes were "function unknown", and may represent unknown aspects of Streptomyces biology. Only two of the 147 genes contain a TTA codon, but some effects of bldA could be traced to TTA codons in regulatory genes or polycistronic operons. Several proteins were affected posttranslationally by the bldA deletion. There was a statistically significant but weak positive global correlation between transcript and corresponding protein levels. Different technical limitations of the two approaches were a major cause of discrepancies in the results obtained with them. Conclusion: Although deletion of bldA has very conspicuous effects on the gross phenotype, the bldA molecular phenotype revealed by the "dualomic" approach has shown that only about 2% of the genome is affected; but this includes many previously unknown effects at a variety of different levels, including post translational changes in proteins and global cellular physiology

Differential Enzymatic ¹⁶O/¹⁸O Labeling for the Detection of Cross-Linked Nucleic Acid-Protein Heteroconjugates

Cross-linking of nucleic acids to proteins in combination with mass spectrometry permits the precise identification of interacting residues between nucleic acid-protein complexes. However, the mass spectrometric identification and characterization of cross-linked nucleic acid-protein heteroconjugates within a complex sample is challenging. Here we establish a novel enzymatic differential O-16/O-18-labeling approach, which uniquely labels heteroconjugates. We have developed an automated data analysis workflow based on OpenMS for the identification of differentially isotopically labeled heteroconjugates against a complex background. We validated our method using synthetic model DNA oligonucleotide-peptide heteroconjugates, which were subjected to the labeling reaction and analyzed by high-resolution FTICR mass spectrometry

Psychological dimensions of retirement.

The chapters that follow examine the character of, and issues relating to, western retirement experiences. As our populations age, issues relating to the nature of retirement are of growing importance. Population ageing is a global issue. For instance, Jacobsen, Kent, Lee, & Mather (2011) report that currently one-fifth of the Japanese population is aged over 65 and estimated to increase to one-third of the population by 2040. Based on Bogomolny’s (2004) calculations, by 2025, there will 2 workers in Japan for every person over 65. By 2030 to 2040, 20% of the United States population (i.e., 70 million people), will be aged over 65 (Conrad Glass & Flynn, 2000; Jacobsen, Kent, Lee & Mather, 2011). A drop in the number of workers per government funded beneficiaries from 3.3. to 2.2 has also been predicted (Social Security Board of Trustees, 2008). Many European countries will have similarly high proportions of their population aged over 65 (Heyma, 2004) with concomitant dependency ratios, as will Australia and New Zealand (Kippen, 2002; Statistics New Zealand, 2012). In the 1970s and 1980s there was a trend toward early retirement, however this began to be reversed in many countries in the 1990s. Participation rates in most OECD countries for older workers (50-64 years) have increased to an average of 63% in 2008. Some countries have seen considerable increases in participation rates for these workers (e.g. New Zealand, Netherlands) and in even older workers (65-69 years) (OECD, 2011). Along with the increasing expansion of working lives has come an evolution of the pathways to retirement. Retirement is no longer necessarily a “clean break” from the workforce, with many researchers arguing that the transition from work to retirement is now “blurred”. Retirement is not a single discrete event but can be viewed as an individual process, where for many paid employment still plays a significant role well into the “third age”. The changing nature of retirement over the past few decades highlights the need to continually reassess how we conceptualise it in the literature and how it impacts on the individual, organisations and society. This book seeks to address some of the psychological dimensions of retirement prominent in the literature. The initial chapter of this book outlines a number of definitions pertinent to the topic of retirement. This is followed by an examination of issues that affect retirement decisions. Next, psychological wellbeing and physical health issues are examined in relation to retirement. The final chapters examine the interplay between work and retirement, the role of leisure in retirement, the experiences of women, and the sources and role of social support in retirement

Mapping protein-DNA interactions using UV cross-linking and mass spectrometry

Protein-nucleic acid interactions play essential roles in all living cells in various cellular functions. The study of these interactions can reveal important structural and functional information. UV cross-linking of nucleic acids to proteins in combination with mass spectrometry is a powerful technique to identify proteins, peptides and the amino acids involved in intermolecular interactions within nucleic acid-protein complexes. However, the mass spectrometric identification of cross-linked nucleic acid-protein heteroconjugates in complex mixtures and MS/MS characterisation of the specific sites of cross-linking is a challenging task. In this investigation, novel tools and methods have been developed for the investigation of DNA-protein interactions using UV cross-linking and mass spectrometry. These tools were developed towards their application for the characterisation of the complex between the eukaryotic DNA repair protein Tyrosyl-DNA phosphodiesterase 1 (Tdp1) and its DNA substrates. DNA-Tdp1 UV cross-linking was optimised using purified recombinant human Tdp1 and radioactively labelled DNA oligonucleotides containing UV photoactivatable 4- thio-thymidine or 5-iodouracil. Tdp1-DNA heteroconjugates were detected by SDS PAGE and Phosphorimaging. In order to analyse the DNA-Tdp1 heteroconjugates by mass spectrometry, they must first be enriched and hydrolysed by a protease and a nuclease. Here, a novel sample preparation protocol was developed for the enrichment of Tdp1 oligonucleotide-peptide heteroconjugates. Detection and analysis of oligonucleotide-peptide heteroconjugates using mass spectrometry is a challenging task. As a tool to optimise the various parameters involved, a synthetic DNA oligonucleotide-peptide heteroconjugate was constructed using click chemistry. RP-HPLC/ESI-FT-ICR-MS on a Bruker 12T SolariX in conjunction with CID fragmentation was used to unambiguously identify the site of the cross-link. Lastly, a novel 18O labeling approach was introduced to facilitate the identification of DNA-protein cross-links. This approach was shown to be suitable for the labeling of heteroconjugate species by testing it with the click heteroconjugate

The effects of professional training on occupational stress and personal affect in rehabilitation practitioners

An exploratory analysis was conducted to determine whether a professional training program for rehabilitation practitioners that focused on core skills and competencies, might have some beneficial effects on perceived levels of job related stress and tension, and feelings of positive and negative affect. Results of the analysis indicated that training participants showed significant improvements in perceptions of job stress, tension, and positive affect compared with a group of control respondents who did not take part in the training. There were no changes in negative affect. Problems and limitations of the study are addressed and the importance of further understanding of the complex dynamics of job stress in rehabilitation professionals is emphasized

Job satisfaction and distress in rehabilitation professionals: The role of organizational commitment and conflict

Examined relations between organizational perceptions (organizational commitment, organizational conflict) and outcomes (job satisfaction, psychological distress) among 82 New Zealand rehabilitation service providers. Analyses indicated that organizational commitment made a significant contribution to the prediction of both job satisfaction and feelings of distress while organizational conflict was a significant predictor of job satisfaction. Age and length of service in the organization was significantly related to job satisfaction while level of education was significantly related to distress