Recent Decisions

Comments on recent decisions by William Fleming, Edward L. Burke, William J. Priebe, Anthony V. Amodio, Richard G. Dytrych, James Kalo, and John A. Pietrykowski

Diversity and recombination in Wolbachia and Cardinium from Bryobia spider mites

Background Wolbachia and Cardinium are endosymbiotic bacteria infecting many arthropods and manipulating host reproduction. Although these bacteria are maternally transmitted, incongruencies between phylogenies of host and parasite suggest an additional role for occasional horizontal transmission. Consistent with this view is the strong evidence for recombination in Wolbachia, although it is less clear to what extent recombination drives diversification within single host species and genera. Furthermore, little is known concerning the population structures of other insect endosymbionts which co-infect with Wolbachia, such as Cardinium. Here, we explore Wolbachia and Cardinium strain diversity within nine spider mite species (Tetranychidae) from 38 populations, and quantify the contribution of recombination compared to point mutation in generating Wolbachia diversity. Results We found a high level of genetic diversity for Wolbachia, with 36 unique strains detected (64 investigated mite individuals). Sequence data from four Wolbachia genes suggest that new alleles are 7.5 to 11 times more likely to be generated by recombination than point mutation. Consistent with previous reports on more diverse host samples, our data did not reveal evidence for co-evolution of Wolbachia with its host. Cardinium was less frequently found in the mites, but also showed a high level of diversity, with eight unique strains detected in 15 individuals on the basis of only two genes. A lack of congruence among host and Cardinium phylogenies was observed. Conclusions We found a high rate of recombination for Wolbachia strains obtained from host species of the spider mite family Tetranychidae, comparable to rates found for horizontally transmitted bacteria. This suggests frequent horizontal transmission of Wolbachia and/or frequent horizontal transfer of single genes. Our findings strengthens earlier reports of recombination for Wolbachia, and shows that high recombination rates are also present on strains from a restrictive host range. Cardinium was found co-infecting several spider mite species, and phylogenetic comparisons suggest also horizontal transmission of Cardinium among host

Genome sequence of the parainfluenza virus 5 strain that persistently infects AGS cells

This work, including the efforts of Richard E Randall, was funded by Wellcome Trust (101788/Z/13/Z). This work, including the efforts of Steve Goodbourn, was funded by Wellcome Trust (101792/Z/13/Z).We have sequenced the parainfluenza virus 5 strain that persistently infects the commonly used AGS human cell line without causing cytopathology. This virus is most closely related to human strains, indicating that it may have originated from biopsy material or from laboratory contamination during generation of the cell line.Publisher PDFPeer reviewe

Former dynamic behaviour of a cold-based valley glacier on Svalbard revealed by basal ice and structural glaciology investigations

H.L. was funded by a UK Natural Environment Research Council (NERC) PhD studentship (NE/I528050/1), the Queen Mary Postgraduate Research Fund, and an Arctic Field Grant from the Research Council of Norway. E.J.F. was funded by a NERC PhD studentship as part of the GAINS (Glacial Activity in Neoproterozoic Svalbard) grant (NE/H004963/1). K.N. was funded by an Arctic Field Grant, the Swiss Society for Speleology, and the travel grant commission of the Swiss Academy of Science.Large numbers of small valley glaciers on Svalbard were thicker and more extensive during the Little Ice Age (LIA), demonstrated by prominent ice-cored moraines up to several kilometres beyond present-day margins. The majority of these glaciers have since experienced a long period of strongly negative mass balance during the 20th century and are now largely frozen to their beds, indicating they are likely to have undergone a thermal transition from a polythermal to a cold-based regime. We present evidence for such a switch by reconstructing the former flow dynamics and thermal regime of Tellbreen, a small cold-based valley glacier in central Spitsbergen, based on its basal sequence and glaciological structures. Within the basal sequence, the underlying matrix-supported diamict is interpreted as saturated subglacial traction till which has frozen at the bed, indicating that the thermal switch has resulted in a cessation of subglacial sediment deformation due to freezing of the former deforming layer. This is overlain by debris-poor dispersed facies ice, interpreted to have formed through strain-induced metamorphism of englacial ice. The sequential development of structures includes arcuate fracture traces, interpreted as shear planes formed in a compressive/transpressive stress regime; and fracture traces, interpreted as healed extensional crevasses. The formation of these sediment/ice facies and structures is indicative of dynamic, warm-based flow, most likely during the LIA when the glacier was significantly thicker.Publisher PDFPeer reviewe

The Importance of Eurekan Mountains on Cenozoic Sediment Routing on the Western Barents She

The importance of topography generated by Eocene Eurekan deformation as a sediment source for sandstones deposited on the western Barents Shelf margin is evaluated through a sediment provenance study conducted on wellbore materials retrieved from Spitsbergen and from the Vestbakken Volcanic Province and the Sørvestsnaget Basin in the southwest Barents Sea. A variety of complementary techniques record a provenance change across the Paleocene-Eocene boundary in wellbore BH 10-2008, which samples Paleogene strata of the Central Tertiary Basin in Spitsbergen. Sandstones containing K-feldspar with radiogenic Pb isotopic compositions, chrome spinel in the heavy mineral assemblage, and detrital zircons and rutiles with prominent Palaeoproterozoic and Late Palaeozoic—Early Mesozoic U-Pb age populations are up-section replaced by sandstone containing albitic plagioclase feldspar, metasedimentary schist rock fragments, a heavy mineral assemblage with abundant chloritoid, metamorphic apatite with low REE contents, metapelitic rutile with Silurian U-Pb ages and zircons with predominantly Archaean and Palaeoproterozoic U-Pb age populations. Our results clearly demonstrate the well-known regional change in source area from an exposed Barents Shelf terrain east of the Central Tertiary Basin during the Paleocene to the emerging Eurekan mountains west and north of the Central Tertiary Basin during the Eocene. Eocene sandstones deposited in the marginal basins of the southwestern Barents Shelf, which were sampled in wellbores 7316/5-1 and 7216/11-1S, contain elements of both the Eurekan and the eastern Barents Shelf provenance signatures. The mixing of the two sand types and delivery to the southwest margin of the Barents Shelf is consistent with a fill and spill model for the Central Teritary Basin, with transport of Eurekan-derived sediment east then south hundreds of kilometres across the Shelf

Debris entrainment and landform genesis during tidewater glacier surges

Funded by: NERC. Grant Number: NE/I528050/1 GAINS (Glacial Activity in Neoproterozoic Svalbard). Grant Number: NE/H004963/1Peer reviewedPublisher PD