Proton therapy for adults with mediastinal lymphomas: The international lymphoma radiation oncology group guidelines

Among adult lymphoma survivors, radiation treatment techniques that increase the excess radiation dose to organs at risk (OARs) put patients at risk for increased side effects, especially late toxicities. Minimizing radiation to OARs in adults patients with Hodgkin and non-Hodgkin lymphomas involving the mediastinum is the deciding factor for the choice of treatment modality. Proton therapy may help to reduce the radiation dose to the OARs and reduce toxicities, especially the risks for cardiac morbidity and second cancers. Becauseproton therapymay have some disadvantages, identifying the patients and the circumstances that may benefit the most from proton therapy is important. We present modern guidelines to identify adult lymphoma patients who may derive the greatest benefit from proton therapy, along with an analysis of the advantages and disadvantages of proton treatment. (Blood. 2018;132(16):1635-1646)

Evidence-based Review on the Use of Proton Therapy in Lymphoma From the Particle Therapy Cooperative Group (PTCOG) Lymphoma Subcommittee.

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Biochemical effects of comlex III Qo site respiratory chain inhibitor kresoxim-methyl on mammalian cells

The ultimate concern over pesticides in the environment is their toxic impact on nontarget organisms, including humans. For over the last two decades, the toxicological research has focused on pesticide-induced oxidative stress in terms of monitoring alterations in various biochemical and molecular compositions. A relative new group of agricultural fungicides, strobilurins, represent a major class of plant protection products and world's largest selling fungicides. They act as complex III Qo site inhibitors and thus disrupting electron transport in the respiratory chain. This electron leakage generates superoxide and potentially results in increased oxidative stress. The present study was aimed at investigating the biochemical mechanisms involved in the toxic action of kresoxim-methyl, a widely used agricultural fungicide of the stobilurins group on murine neuroblastoma cells (N2a)

Nafion and Polylysine treated PEDOT mammalian cell biosensor [poster]

The present study describes a cell-based biosensor utilizing PEDOT electrodes coated with Nafion and Polylysine for combined conductivity, cellular adhesion and proliferation. Neuroblastoma N2a cells were seeded on top of PEDOT electrodes treated with Nafion and Polylysine. Cellular attachment and viability were assayed and chronoamperometric measurements were taken to evaluate H 202 toxicity. Cells exhibited relatively hight viability compared to those seeded in tissue culture plates. Chronoamperometric responses also provided preliminary evidence of the possible use of this assembly as a toxicity biosensor

Study of a cell-based electrochemical biosensor for fungicide cytotoxicity evaluation on mammalian cell lines

The ultimate concern over pesticides in the environment is their toxic impact on nontarget organisms, including humans. For over the last two decades, the toxicological research has focused on pesticide-induced oxidative stress in terms of monitoring alterations in various biochemical and molecular compositions. A relative new group of agricultural fungicides, strobilurins, represent a major class of plant protection products and world's largest selling fungicides. They act as complex III Qo site inhibitors and thus disrupt electron transport in the respiratory chain, which generates superoxide and potentially results in oxidative stress. Cell-based biosensors arise as powerful tools for the rapid detection of xenobiotics in food industries, agriculture and the environment. The present study was aimed at developing an electrochemical biosensor for cytotoxicity assays on mammalian cells, cultured on PEDOT electrodes. The cellular mono layers formed on top of the conductive material, support cell interactions, growth and metabolism as cells keep their original characteristics of morphology and functionality. In this framework, the action of kresoxim-methyl, of the stobilurins group, was examined on murine neuroblastoma cells (N2a). Square wave voltammetry (SWV) and a three-electrode configuration (W: PEDOT, C: Carbon, R: Silver) were used for recording the electrochemical changes after drug treatment. Moreover, standard viability/cytotoxicity protocols were conducted as references for assay comparisons. Due to PEDOTs transparent composition morphological observations and adhesion tests of the seeded cells were also made. Electrochemical responses against the fungicide provided evidence of the possible use of this assembly as a toxicity biosensor