Seismicity and geometry of the south Chilean subduction zone (41.5°S-43.5°S): Implications for controlling parameters

In 2005 an amphibious seismic network was deployed on the Chilean forearc between 41.75°S and 43.25°S. 364 local events were observed in a 11-month period. A subset of the P and S arrival times were inverted for hypocentral coordinates, 1-D velocity structure and station delays. Main seismic activity occurred predominantly in a belt parallel to the coast of Chiloé Island in a depth range of 12–30 km presumably related to the plate interface. The 30° inclination of the shallow part of the Wadati-Benioff zone is similar to observations further north indicating that oceanic plate age is not controlling the subduction angle of the shallower part for the Chilean subduction zone. The down-dip termination of abundant intermediate depth seismicity at approximately 70 km depth seems to be related to the young age (and high temperature) of the oceanic plate. Crustal seismicity is associated with the Liquiñe-Ofqui fault zone and active volcanoes

let-7 microRNAs regulate microglial function and suppress glioma growth through Toll-like receptor 7

Microglia express Toll-like receptors (TLRs) that sense pathogen- and host-derived factors, including single-stranded RNA. In the brain, let-7 microRNA (miRNA) family members are abundantly expressed, and some have recently been shown to serve as TLR7 ligands. We investigated whether let-7 miRNA family members differentially control microglia biology in health and disease. We found that a subset of let-7 miRNA family members function as signaling molecules to induce microglial release of inflammatory cytokines, modulate antigen presentation, and attenuate cell migration in a TLR7-dependent manner. The capability of the let-7 miRNAs to control microglial function is sequence specific, mapping to a let-7 UUGU motif. In human and murine glioblastoma/glioma, let-7 miRNAs are differentially expressed and reduce murine GL261 glioma growth in the same sequence-specific fashion through microglial TLR7. Taken together, these data establish let-7 miRNAs as key TLR7 signaling activators that serve to regulate the diverse functions of microglia in health and glioma

Microglia/macrophage derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model

Factors released from glioma-associated microglia/macrophages (GAMs) play a crucial role in glioblastoma multiforme (GBM) progression. Here, we study the importance of CCL18, a cytokine expressed in human but not in rodent GAMs, as a modulator of glioma growth. Since CCL18 signaling could not be studied in classical mouse glioma models, we developed an approach by transplanting induced pluripotent stem cell-derived human microglia and human glioma cells into mouse brain slices depleted of their intrinsic microglia. We observe that CCL18 promotes glioma cell growth and invasion. Chemokine (C-C motif) receptor 8 (CCR8) is identified as a functional receptor for CCL18 on glioma cells, and ACP5 (acid phosphatase 5) is revealed as an important part of the downstream signaling cascade for mediating glioma growth. We conclude, based on the results from an in vitro, ex vivo humanized glioma model and an in vivo GBM model that microglia/macrophage-derived CCL18 promotes glioma growth

The ocean-bottom seismometer of the German instrument pool for amphibian seismology (DEPAS)

In this contribution we present the the ocean-bottom seismometer of the German instrument pool for amphibian seismology (DEPAS). Technical specifications of the instruments are introduced and illustrated by data examples