DNA Interaction with Palladium Chelates of Biogenic Polyamines Using Atomic Force Microscopy and Voltammetric Characterization

The interaction of double-stranded DNA with two polynuclear Pd(II) chelates with the biogenic polyamines spermidine (Spd) and spermine (Spm), Pd(II)-Spd and Pd(II)-Spm, as well as with the free ligands Spd and Spm, was studied using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite (HOPG) surface, voltammetry at a glassy carbon (GC) electrode, and gel electrophoresis. The AFM and voltammetric results showed that the interaction of Spd and Spm with DNA occurred even for a low concentration of polyamines and caused no oxidative damage to DNA. The Pd(II)-Spd and Pd(II)-Spm complexes were found to induce greater morphological changes in the dsDNA conformation, when compared with their ligands. The interaction was specific, inducing distortion and local denaturation of the B-DNA structure with release of some guanine bases. The DNA strands partially opened give rise to palladium intra- and interstrand cross-links, leading to the formation of DNA adducts and aggregates, particularly in the case of the Pd(II)-Spd complex

One-to-one full scale simulations of laser wakefield acceleration using QuickPIC

We use the quasi-static particle-in-cell code QuickPIC to perform full-scale, one-to-one LWFA numerical experiments, with parameters that closely follow current experimental conditions. The propagation of state-of-the-art laser pulses in both preformed and uniform plasma channels is examined. We show that the presence of the channel is important whenever the laser self-modulations do not dominate the propagation. We examine the acceleration of an externally injected electron beam in the wake generated by 10 J laser pulses, showing that by using ten-centimeter-scale plasma channels it is possible to accelerate electrons to more than 4 GeV. A comparison between QuickPIC and 2D OSIRIS is provided. Good qualitative agreement between the two codes is found, but the 2D full PIC simulations fail to predict the correct laser and wakefield amplitudes.Comment: 5 pages, 5 figures, accepted for publication IEEE TPS, Special Issue - Laser & Plasma Accelerators - 8/200

Collisionless shock acceleration of narrow energy spread ion beams from mixed species plasmas using 1 μ\mum lasers

Collisionless shock acceleration of protons and C$^{6+}$ ions has been achieved by the interaction of a 10$^{20}$ W/cm$^2$, 1 $\mu$m laser with a near-critical density plasma. Ablation of the initially solid density target by a secondary laser allowed for systematic control of the plasma profile. This enabled the production of beams with peaked spectra with energies of 10-18 MeV/a.m.u. and energy spreads of 10-20$\%$ with up to 3x10$^9$ particles within these narrow spectral features. The narrow energy spread and similar velocity of ion species with different charge-to-mass ratio are consistent with acceleration by the moving potential of a shock wave. Particle-in-cell simulations show shock accelerated beams of protons and C$^{6+}$ ions with energy distributions consistent with the experiments. Simulations further indicate the plasma profile determines the trade-off between the beam charge and energy and that with additional target optimization narrow energy spread beams exceeding 100 MeV/a.m.u. can be produced using the same laser conditions.Comment: Accepted for publication in Physical Review Accelerators and Beam

Development of an Interpretive Simulation Tool for the Proton Radiography Technique

Proton radiography is a useful diagnostic of high energy density (HED) plasmas under active theoretical and experimental development. In this paper we describe a new simulation tool that interacts realistic laser-driven point-like proton sources with three dimensional electromagnetic fields of arbitrary strength and structure and synthesizes the associated high resolution proton radiograph. The present tool's numerical approach captures all relevant physics effects, including effects related to the formation of caustics. Electromagnetic fields can be imported from PIC or hydrodynamic codes in a streamlined fashion, and a library of electromagnetic field `primitives' is also provided. This latter capability allows users to add a primitive, modify the field strength, rotate a primitive, and so on, while quickly generating a high resolution radiograph at each step. In this way, our tool enables the user to deconstruct features in a radiograph and interpret them in connection to specific underlying electromagnetic field elements. We show an example application of the tool in connection to experimental observations of the Weibel instability in counterstreaming plasmas, using $\sim 10^8$ particles generated from a realistic laser-driven point-like proton source, imaging fields which cover volumes of $\sim10$ mm$^3$. Insights derived from this application show that the tool can support understanding of HED plasmas.Comment: Figures and tables related to the Appendix are included in the published journal articl

Extreme Plasma Astrophysics

This is a science white paper submitted to the Astro-2020 and Plasma-2020 Decadal Surveys. The paper describes the present status and emerging opportunities in Extreme Plasma Astrophysics -- a study of astrophysically-relevant plasma processes taking place under extreme conditions that necessitate taking into account relativistic, radiation, and QED effects.Comment: A science white paper submitted to the Astro-2020 and Plasma-2020 Decadal Surveys. 7 pages including cover page and references. Paper updated in late March 2019 to include a several additional co-authors and references, and a few small change

Composição de raízes de mandioca em três épocas de colheita.

A cultura da mandioca é de grande relevância socioeconômica para o Brasil, apresentando as características de facilidade de cultivo e resistência a doenças e a variações climáticas. Com a raiz da mandioca pode-se preparar vários tipos de alimentos, sendo o teor de compostos cianogênicos um dos fatores que definem a finalidade de uso, classificando as variedades em ?mansas? e ?bravas?

Evaluation of carotenoid and cyanogen content of cassava chip flour.

Large genetic variation in carotenoid content has been reported to roots of cassava genotypes.Moreover, these pigments have to withstand different processing methods before cassava is consumed

Selection of sweet cassava accessions with high content of carotenoids.

Cassava is a drought-tolerant, staple food crop grown in tropical and subtropical areas where many people are afflicted with undernutrition, making it a potentially valuable food source for developing countries. Efforts to add nutritional value to cassava by increasing the contents of protein and carotenoid are underway