TreeML_EF

ML tree from the analysis of EF sequence

Tables S1-S3 : Ficus genotypes

Table S1 Genotypes of parents and offspring in controlled crosses. Table S2 Parent and offspring probability of assignation to genetic cluster using Structure. Table S3 Genotypes at the 7 retained loci of 355 individuals used for the global analysis

TreeML_Cytb

ML tree from the analysis of Cytb sequence

Ficus plastid genome alignment

MAFFT alignment of 59 near-complete Ficus plastid genomes and 6 Castilleae plastid genomes as outgroup produced by high throughput sequencing

Dataset_Cytb

Cytb sequences for phylogenetic inference (Agaonidae

Bayesian majority-rule consensus tree reconstructed from near-complete plastome sequences for 59 species of Ficus and outgroup.

Bayesian majority-rule consensus tree depicted in Figure 1 and described in publication

Daily Rhythm of Mutualistic Pollinator Activity and Scent Emission in <i>Ficus septica</i>: Ecological Differentiation between Co-Occurring Pollinators and Potential Consequences for Chemical Communication and Facilitation of Host Speciation

<div><p>The mutualistic interaction between <i>Ficus</i> and their pollinating agaonid wasps constitutes an extreme example of plant-insect co-diversification. Most <i>Ficus</i> species are locally associated with a single specific agaonid wasp species. Specificity is ensured by each fig species emitting a distinctive attractive scent. However, cases of widespread coexistence of two agaonid wasp species on the same <i>Ficus</i> species are documented. Here we document the coexistence of two agaonid wasp species in <i>Ficus septica</i>: one yellow-colored and one black-colored. Our results suggest that their coexistence is facilitated by divergent ecological traits. The black species is longer-lived (a few more hours) and is hence active until later in the afternoon. Some traits of the yellow species must compensate for this advantage for their coexistence to be stable. In addition, we show that the composition of the scent emitted by receptive figs changes between sunrise and noon. The two species may therefore be exposed to somewhat different ranges of receptive fig scent composition and may consequently diverge in the way they perceive and/or respond to scents. Whether such situations may lead to host plant speciation is an open question.</p></div

Refined PERMANOVA analysis on the relative composition of scents emitted by figs of <i>Ficus septica</i> and of <i>Ficus nota</i> at sunrise and at noon.

<p>Refined PERMANOVA analysis on the relative composition of scents emitted by figs of <i>Ficus septica</i> and of <i>Ficus nota</i> at sunrise and at noon.</p

Colonization by pollinators of <i>Ficus septica</i> receptive figs whose accessibility has been manipulated.

<p>Number of yellow (light grey) and black (dark grey) foundresses found inside figs that have been left accessible to pollination for the whole day or in the afternoon only. Raw data provided as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103581#pone.0103581.s002" target="_blank">Table S2</a>.</p

Daily variation of scent emission rates in <i>Ficus septica</i> and <i>Ficus nota</i>.

<p>Total scent emission rates from (A) figs (µg/fig*hour) and (B) leaves (µg/cm²*hour) of both species at sunrise and at noon.</p