Development of digital computer program for thermal network correction. Phase 2 - Program development. Phase 3 - Demonstration/application Final report

Developing digital computer program for correcting soft parameters of thermal network by Kalman filtering metho

Aerodynamic data on a large semispan tilting wing with 0.5-diameter chord, double-slotted flap, and both left-hand and right-hand rotation of a single propeller

Longitudinal aerodynamic data on large-scale semispan V/STOL tilt-wing configuration having single propeller with left and right hand rotatio

Photon State Tomography for Two-Mode Correlated Itinerant Microwave Fields

Continuous variable entanglement between two modes of a radiation field is usually studied at optical frequencies. As an important step towards the observation of entanglement between propagating microwave photons we demonstrate the experimental state reconstruction of two field modes in the microwave domain. In particular, we generate two-mode correlated states with a Josephson parametric amplifier and detect all four quadrature components simultaneously in a two-channel heterodyne setup using amplitude detectors. Analyzing two-dimensional phase space histograms for all possible pairs of quadratures allows us to determine the full covariance matrix and reconstruct the four-dimensional Wigner function. We demonstrate strong correlations between the quadrature amplitude noise in the two modes. Under ideal conditions two-mode squeezing below the standard quantum limit should be observable in future experiments.Comment: 4 pages, 4 figure

SP-Sephadex equilibrium chromatography of bradykinin and related peptides: Application to trypsin-treated human plasma

An analytical method is deseribed for the separation of bradykinin, Lys-bradykinin, and Met-Lys-bradykinin by equilibrium chromatography on SP-Sephadex C-25 eluted in 0.02 Tris-HCl buffer, pH 8.10, 0.12 NaCl. A second elution buffer, 0.02 Tris-HCl buffer, pH 7.70, 0.06 NaCl, serves as a second parameter for the identification of bradykinin and also separates the hormone from plasma bradykinin-potentiating peptides. Ten to one-hundred nanomoles of each peptide can be recovered in high yields, identified by elution position, and measured by bioassay with the isolated guinea pig ileum. The identification of bradykinin as the peptide released by trypsin acting on acid-denatured plasma is documented as an illustration of the method

Universal Tutte characters via combinatorial coalgebras

The Tutte polynomial is the most general invariant of matroids and graphs that can be computed recursively by deleting and contracting edges. We generalize this invariant to any class of combinatorial objects with deletion and contraction operations, associating to each such class a universal Tutte character by a functorial procedure. We show that these invariants satisfy a universal property and convolution formulae similar to the Tutte polynomial. With this machinery we recover classical invariants for delta-matroids, matroid perspectives, relative and colored matroids, generalized permutohedra, and arithmetic matroids, and produce some new convolution formulae. Our principal tools are combinatorial coalgebras and their convolution algebras. Our results generalize in an intrinsic way the recent results of Krajewski--Moffatt--Tanasa.Comment: Accepted version, 51p

Eluate derived by extracorporal antibody-based immunoadsorption elevates the cytosolic Ca2+ concentration in podocytes via B-2 kinin receptors

Background/Aim: Patients with idiopathic focal segmental glomerulosclerosis (FSGS) often develop a recurrence of the disease after kidney transplantation. In a number of FSGS patients, plasmapheresis and immunoadsorption procedures have been shown to transiently reduce proteinuria and are thought to do this by eliminating a circulating factor. Direct cellular effects of eluates from immunoadsorption procedures on podocytes, the primary target of injury in FSGS, have not yet been reported. Methods: Eluates were derived from antibody-based immunoadsorption of a patient suffering from primary FSGS, a patient with systemic lupus erythematosus, and a healthy volunteer. The cytosolic free Ca2+ concentration ({[}Ca2+](i)) of differentiated podocytes was measured by single-cell fura-2 microfluorescence measurements. Free and total immunoreactive kinin levels were measured by radioimmunoassay. Results: FSGS eluates increased the {[}Ca2+](i) levels concentration dependently (EC50 0.14 mg/ml; n = 3-19). 1 mg/ml eluate increased the {[}Ca2+](i) values reversibly from 82 +/- 12 to 1,462 +/- 370 nmol/l, and then they returned back to 100 16 nmol/l (n = 19). The eluate-induced increase of {[}Ca2+](i) consisted of an initial Ca2+ peak followed by a Ca2+ plateau which depended on the extracellular Ca2+ concentration. The eluate-induced increase of {[}Ca2+](i) was inhibited by the specific B-2 kinin receptor antagonist Hoe 140 in a concentration-dependent manner (IC50 2.47 nmol/l). In addition, prior repetitive application of bradykinin desensitized the effect of eluate on {[}Ca2+](i). A colonic epithelial cell line not reacting to bradykinin did not respond to eluate either (n = 6). Similar to FSGS eluates, the eluate preparations of both the systemic lupus patient and the healthy volunteer led to a biphasic, concentration-dependent {[}Ca2+](i) increase in poclocytes which again was inhibited by Hoe 140. Free kinins were detected in all eluate preparations. Conclusion: The procedure of antibody-based immunoadsorption leads to kinin in the eluate which elevates the {[}Ca2+](i) level of podocytes via B-2 kinin receptors. Copyright (C) 2002 S. Karger AG, Basel