Antibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile mice

Antibiotic use during adolescence may result in dysbiosis-induced neuronal vulnerability both in the enteric nervous system (ENS) and central nervous system (CNS) contributing to the onset of chronic gastrointestinal disorders, such as irritable bowel syndrome (IBS), showing significant psychiatric comorbidity. Intestinal microbiota alterations during adolescence influence the expression of molecular factors involved in neuronal development in both the ENS and CNS. In this study, we have evaluated the expression of brain-derived neurotrophic factor (BDNF) and its high-affinity receptor tropomyosin-related kinase B (TrkB) in juvenile mice ENS and CNS, after a 2-week antibiotic (ABX) treatment. In both mucosa and mucosa-deprived whole-wall small intestine segments of ABX-treated animals, BDNF and TrKB mRNA and protein levels significantly increased. In longitudinal muscle-myenteric plexus preparations of ABX-treated mice the percentage of myenteric neurons staining for BDNF and TrkB was significantly higher than in controls. After ABX treatment, a consistent population of BDNF-and TrkB-immunoreactive neurons costained with SP and CGRP, suggesting up-regulation of BDNF signaling in both motor and sensory myenteric neurons. BDNF and TrkB protein levels were downregulated in the hippocampus and remained unchanged in the prefrontal cortex of ABX-treated animals. Immunostaining for BDNF and TrkB decreased in the hippocampus CA3 and dentate gyrus subregions, respectively, and remained unchanged in the prefrontal cortex. These data suggest that dysbiosis differentially influences the expression of BDNF-TrkB in the juvenile mice ENS and CNS. Such changes may potentially contribute later to the development of functional gut disorders, such as IBS, showing psychiatric comorbidity

La forma del piano e le sue componenti

iL TESTO CONTIENE RIFLESSIONI IN MERITO AI REQUISITI POSTI ALLA BASE DEL PS DI SIENA, ED IN PARTICOLARE ALLA CHIAREZZA DEL LINGUAGGIO, ALLA CAPACITà DI INTERAGIRE CON ALTRI STRUMENTI DI PIANIFICAZIONE, ALLA CAPACITà DI CONTROLLARE I PROCESSI DI TRASFORMAZIONE EDILIZI ED URBANI, ALLA COERENZA CON LE SCELTE DI AREA VASTAREFLECTION ABOUT THE CONTENTS OF THE SP OF SIENA, NAMELY THE CLARITY OF THE LANGUAGE, THE CAPACITY TO INTERACT WITH OTHER PLANNING INSTRUMENTS, THE CAPACITY TO CONTROL THE QUALITY OF THE TOWN PLANNING AND ARCHITECTURAL TRANSFORMATINS, ITS COHERENCY WITH THE CHOICES OF THE VAST AREA GOVERNANC

UNA PROCEDURA PER LA VALUTAZIONE TERRITORIALE DEI PROGETTI: IL MODELLO VATE

Vengono illustrate le caratteristiche e le prestazioni del D.D.S. (Decision Support System) "Vate", software implementato nel corso della ricerca "La valutazione territoriale dei progetti" ed utilizzabile per ordinare in termini di priorità (in funzione di un "set" di obiettivi di programmazione) un determinato parco-progetti da realizzare