24 research outputs found
Modelagem da Lagoa da Pampulha: uma ferramenta para avaliar o impacto da bacia hidrográfica na dinâmica do fitoplâncton
Long-range Angular Correlations On The Near And Away Side In P-pb Collisions At √snn=5.02 Tev
7191/Mar294
Impacts of different management systems on the physical quality of an Amazonian Oxisol
Response of phytoplankton to an experimental fish culture in net cages in a subtropical reservoir
Detection of glycidic receptors in microalgae using glycodendrons as probes: a new tool for studies on cell surface interactions
Cell recognition, adhesion, and internalization are involved in infectious, reproductive, and inflammatory processes and are generally mediated by interactions between molecules located in the cell membrane and the extracellular matrix. These processes can decrease proliferation rates and they are well known for bacteria, fungi, and animals, but there is a lack of knowledge regarding autotrophic cells. Carbohydrates and proteins (e.g., lectins) are important molecules for cell interactions and information about these molecules is essential to better understand many biological phenomena in uni- or multicellular organisms. Most studies focus on the identification of the carbohydrates present on the cell surface by using labeled lectins. Alternatively, here we present a pioneer research performed by using three different labeled carbohydrates in a multivalent presentation (glycodendrons) to detect the presence of carbohydrate receptors (e.g., lectins) on cell surfaces of 12 algal species. The goal of this study was to detect some specificity in these molecular interactions, but in a reverse way in comparison to that commonly described in the literature. We tested trivalent molecules containing residuals of D-mannose, L-fucose, or N-acetyl-galactosamine to identify their bindings with the corresponding lectins expressed on cell surfaces. We envisage that our new approach could be an alternative tool for taxonomic and physiological studies on microalgae or even on other groups of organisms. Based on our results, the receptors found in the cell surface of the algal species tend to differ in composition, quantity, and distribution. The differences were mainly species-specific, since no patterns were identified at higher taxonomic level. Moreover, like lectins, labeled carbohydrates were proved to be a reliable tool for the study of cell surface composition