Identification of novel genetic variants for KAP1.1, KAP1.3 and K33 genes in some of indigenous goat breeds of Turkey

The animal fibres such as mohair, cashmere and cashgora have a complex structure and affected by genetic variation of keratin associated protein genes as KAP 1.1 (Keratin Associated Protein 1.1, formerly known as B2A), KAP1.3 (Keratin Associated Protein 1.3, formerly known as B2C) and K33 (Keratin Intermediate Filaments Type I, formerly known as KRT1.2). Keratin-associated proteins play a significant role in identifying structural and mechanical properties of the hair and wool fibres. This study was conducted to detect genetic variation at the KAP1.1, KAP1.3 and K33 genes in indigenous Turkish goat populations using DNA sequencing method. The DNA of 100 individuals selected from 5 different native goat breeds (Hair, Honamli, Kilis, Norduz, and Angora) that reared different regions of Turkey were used as materials. A total of 59 nucleotide variations and indels (insertion/deletion) of KAP1.1 gene, 15 nucleotide variations and indels of KAP1.3 gene, 16 nucleotide variations of K33 gene were determined in the studied samples. These nucleotide variations and indels have been causing changes in the number and sequence of amino acids. It is necessary to determine the relationships with mohair yield, quality and polymorphisms that are determined in KAP1.1, KAP1.3 and KRT1.2 genes

An investigation of polymorphism kap 1.1 and kap 1.3 genes in some indigenous goat breeds

Bu çalışmada, Türkiye yerli keçi popülasyonlarında KAP1.1 ve KAP1.3 gen bölgelerinde bulunan genetik varyasyon DNA dizileme yöntemleri kullanılarak belirlenmiştir. Çalışmada, Türkiye'nin farklı bölgelerinde yetiştiriciliği yapılan 5 farklı yerli keçi ırkına ait popülasyonlardan seçilen 100 bireyin DNA'sı materyal olarak kullanılmıştır. Tez kapsamında incelenen örneklerde KAP1.1 geni için 59, KAP1.3 geni için 15 noktada seçilen popülasyonlar arasında tek nükleotid değişimi ve insersiyon/delesyon bölgesi belirlenmiştir. Bu nükleotid değişimlerinin ve insersiyon/delesyonların aminoasit sırasında ve sayısında değişime neden olduğu saptanmıştır. Kıl-Tiftik verim ve kalitesini etkilediği düşünülen KAP1.1 ve KAP1.3 genlerinde belirlenen polimorfizlerin marker destekli seleksiyon çalışmalarında kullanılabilmesi için verim özellikleri ile ilişkilerinin tanımlanması gerekmektedir. Ancak bu konuda yapılmış çalışma sayısının azlığı nedeniyle, yerli keçi ırklarımızda hem genetik varyasyonun tanımlanmasına yönelik hem de verim özellikleri ile ilişkilerinin belirlenmesine yönelik çalışmaların yapılması önerilmektedir.In this study, KAP1.1 and KAP1.3 genes were identified the genetic variation of Turkish indigenous goat populations by using DNA sequence method. In the study, DNA of 100 individuals selected from populations belonging to 5 different native goat breeds that reared different regions of Turkey was used as material. In the samples examined within the scope of the thesis, a single nucleotide exchange and insertion / deletion region were determined between the populations selected at 59 points for the KAP1.1 gene and 15 points for the KAP1.3 gene. These nucleotide variations and insertions/deletions have been causing the changes in the number and sequence of amino acids. It is necessary to define the relationship with yield characteristics and polymorphisms that determined in KAP1.1 and KAP1.3 genes which are thought to affect mohair quality in order to use in marker assisted selection studies. However, due to the limited number of studies on this subject, it is recommended to conduct studies both for the identification of genetic variation in Turkish indigenous breeds and for determining their relationship with yield characteristics