Pengaruh Tripton Dan Arang Aktif Pada Pembesaran Bibit Anggrek Phalaenopsis in Vitro

The research was aimed to discover the influence of tripton and active carbon on faster orchid Phalaenopsis in vitro seedling enlargement and to obtain the best media that will spur the enlargement of orchid Phalaenopsis in vitro seedling. The research was using completely randomized design with 8 treatments and 4 replications, and then analyzing the data by using analyze of variant that continued with 5% LSD test. The treatments of this research were : ToAo: Tripton 0 g.l-1 + without active carbon, ToA2: Tripton 0 g.l-1 + active carbon 2 g.l-1 , T1Ao: Tripton 1 g.l-1 + without active carbon, T1A2 : Tripton 1 g.l-1 + active carbon 2 g.l-1 , T2Ao: Tripton 2 g.l-1 + without active carbon, T2A2 : Tripton 2 g.l-1 + active carbon 2 g.l-1 , T3Ao: Tripton 3 g.l-1 + without active carbon, T3 A2: Tripton 3 g.l-1 + active carbon 2 g.l-1 . Basic media that used in this research was foliar fertilizer vitabloom media 2 g.l-1 given vitamin B-1 technique 1 cc.l-1, sugar 20 g.l-1 , tomatoes 60 g.l-1, agar 7 g.l-1, coconut water 150 cc.l-1. The result of this research can be concluded that (1) the influence of tripton and active carbon on orchid phaleonopsis seedling show significant effect on seedling length and the width of leaf, whereas the number of leaf , the number of root, root length and seedling wet weight were not showing significant difference, (2) Media treatment with tripton and active carbon (T1A2) is better in accelerating growth of seedling length and leaf width

Pengaruh Pupuk Daun Dan Arang Aktif Pada Media Subkultur II Terhadap Pertumbuhan Bibit Anggrek Phalaenopsis

The purpose of this study is to obtain the best media as media subculture II in accelerating the growth of Phalaenopsis orchid seedlings with foliar fertilizers as well as the addition of activated charcoal. Research using completely randomized design with 10 treatments and 3 replication. Data were analyzed with analysis of variance and followed by LSD test at 5% level. Treatment reseach is: KH2A0: Kristalon green 2 g.l-1+ Without activated charcoal, KH2A1/2 : Kristalon green 2 g.l-1+ Activated charcoal ½ g.l-1,KH2A1: Kristalon green 2 g.l-1+ Activated charcoal g.l-1 , KH 2A11/2 : Kristalon green 2 g.l-1 + Activated charcoal 1,5 g.l-1, KH 2A2 : Kristalon green 2 g.l-1 + Activated charcoal 2 g.l-1 , Vb2A0 : Vitabloom 2g.l-1 + Without activated charcoal, Vb2A1/2 : Vitabloom 2 g.l-1 + Activated charcoal ½ g.l-1, Vb2A1: Vitabloom 2 g.l-1 + Activated charcoal 1 g.l-1, Vb2A11/2: Vitabloom 2 g.l-1 + Activated charcoal 1,5 g.l-1, Vb2A2: Vitabloom 2 g.l-1 + Activated charcoal 2 g.l-1. The results obtained; (1)) The use of foliar fertilizers vitabloom 2 g.l-1 with the addition of activated charcoal 1,5 g.l-1 showed the best response by producing the highest plant height althought not substantially different with the addition of activated charcoal ½ g.l-1and 1 g.l-1 and the biggest wet crop weight. (2) Media vitabloom fertilizers and without activated charcoal shows a poor response

Pengaruh Pupuk Daun Terhadap Pertumbuhan Bibit Kompot Anggrek Dendrobium Hasil Silangan

The objective of this second year research was to find foliar fertilizer in stimulating the growth of community pot seedling of Dendrobium which was cross pollinated on the first year. The research was conducted in a Completely Randomaized Design with 11 treatmets and 4 replication. The treatments were: vitabloom (P1), gandasil D (P2), plant catalyst (P3), dekastar (P4), red hyponex (P5), vitabloom+dekastar (P6), vitabloom+red hyponex (P7), gandasil D+dekastar(P8), gandasil D+red hyponex P(9), plant catalyst+dekastar (P10), plant catalyst+red hyponex (P11). The results showed that (1) Vitabloom (VB) application gave a better growth of dendrobium seedling (fresh weight, colour and vigor), followed by vitabloom combined with dekastar (VB DS), and dekastar (DS); (2) Plant catalys (PC) gave the lowest growth of seedling, otherwise, combined with red hyponex (PC HM); and (3) Applicaton of higher N content fertilizers (vitabloom, and dekastar or its combination) showed a better and faster growth of seedling compared with applicaton of lower N content fertilizers (plant catalyst)

In vitro regeneration of porang (Amorphophallus muelleri Blume) at several concentrations of BAP (benzyl amino purine)

There is problem about propagating porang by seed that caused it is not always available. Planting by bulbs, bulbil, or leaf cuttings need longer times caused by dormancy problems. Micropropagation by tissue culture is an alternative method that can be choose to fix the problem. This research is aim to obtain the best concentration of BAP to regenerate ‘Madiun 1’ variety of porang plants by shoots (organogenesis) formation. The research was carried out using complete randomized design with 7 treatments concentrations of BAP, that was: P1 (0 mg l-1 BAP), P2 (1 mg l-1 BAP), P3 (2 mg l-1 BAP), P4 (3 mg l-1 BAP), P5 (4 mg l-1 BAP), P6 (5 mg l-1 BAP), and P7 (6 mg l-1 BAP). Each treatment was consisted of 5 replications. Observation were made on number of budding, number of shoots, and shoot height. The data were analyzed using ANOVA, and the differences between treatments were tested using the LSD test at the 5% level. The results revealed that 2 mg l-1 (P3) was the best concentration of BAP to be added to the media to regenerate katak porang tubers of Porang Madiun 1 by in vitro. &nbsp