Distribution of annual rainfall in homogeneous regions through data mining techniques in southern Brazil.

This study aimed to transform time series of annual data of precipitation of Rio Grande do Sul in homogeneous precipitation regions through clustering techniques (data mining).Resumo em português: Distribuição da precipitação pluvial anual em regiões homogêneas por meio de mineração de dados, no sul do Brasil. SIC 2015

ESTAÇÕES agrometeorológicas automáticas.

As estações agrometeorologicas automaticas tem por finalidade monitorar as condições climáticas, permitindo quantificar a evapotranspiração de referência (ETo) e a precipitação pluviométrica, elementos climáticos indispensáveis para o manejo da irrigação das culturas. Equipe técnica: Aderson Soares de Andrade Júnior, Edson Alves Bastos, Valber Mendes Ferreira e Antonio Guaraná Mendes.bitstream/CPAMN-2009-09/22247/1/estacoes_agromet_automatic.pd

Cyclobenzaprine raises ROS levels in Leishmania infantum and reduces parasite burden in Infected mice

11 p.-5 fig. Ferreira Cunha-Júnior, Edézio et al.Background The leishmanicidal action of tricyclic antidepressants has been studied and evidences have pointed that their action is linked to inhibition of trypanothione reductase, a key enzyme in the redox metabolism of pathogenic trypanosomes. Cyclobenzaprine (CBP) is a tricyclic structurally related to the antidepressant amitriptyline, differing only by the presence of a double bond in the central ring. This paper describes the effect of CBP in experimental visceral leishmaniasis, its inhibitory effect in trypanothione reductase and the potential immunomodulatory activity.Methodology/Principal Findings In vitro antileishmanial activity was determined in promastigotes and in L. infantum-infected macrophages. For in vivo studies, L. infantum-infected BALB/c mice were treated with CBP by oral gavage for five days and the parasite load was estimated. Trypanothione reductase activity was assessed in the soluble fraction of promastigotes of L. infantum. For evaluation of cytokines, L. infantum-infected macrophages were co-cultured with BALB/c splenocytes and treated with CBP for 48 h. The supernatant was analyzed for IL-6, IL-10, MCP-1, IFN-γ and TNF-α. CBP demonstrated an IC50 of 14.5±1.1μM and an IC90 of 74.5±1.2 μM in promastigotes and an IC50 of 12.6±1.05 μM and an IC90 of 28.7±1.3 μM in intracellular amastigotes. CBP also reduced the parasite load in L. infantum-infected mice by 40.4±10.3% and 66.7±10.5% in spleen at 24.64 and 49.28 mg/kg, respectively and by 85.6±5.0 and 89.3±4.8% in liver at 24.64 and 49.28mg/kg, after a short-term treatment. CBP inhibited the trypanothione reductase activity with a Ki of 86 ± 7.7 μM and increased the ROS production in promastigotes. CBP inhibited in 53% the production of IL-6 in infected macrophages coculture.Conclusion/Significance To the best of our knowledge, this study is the first report of the in vivo antileishmanial activity of the FDA-approved drug CBP. Modulation of immune response and induction of oxidative stress in parasite seem to contribute to this efficacy.This work was supported by Programa Estratégico de Apoio à Pesquisa em Saúde,FIOCRUZ/Conselho Nacional de Desenvolvimento Científico e Tecnológico - www.cnpq.br, (PAPES/ CNPq 407680/2012-8 to ECTS and 407590/2012-9 to EEAA), Fundação de Apoio a Pesquisa do Estado do Rio de Janeiro ± www.faperj.br (Fellow and grant E-26/010.001828/2016 to EFCJ), Conselho Nacional de Desenvolvimento CientõÂfico e TecnoloÂgico (CNPq/Universal grant 470627/2013-1 to EEAA), São Paulo Research Foundation - www.fapesp.br (FAPESP 2015/23403-9 to AGT),Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad FEDER - www.idi.mineco.gob.es/ (SAF2015-65740-R) and Subdirección General de Redes y Centros de Investigación Cooperativa-FEDER -www.isciii.es/ (RD12/0018/0007) (to LR).Peer reviewe

Inoculação de genótipos de feijão carioca com bactérias diazotróficas.

O objetivo deste trabalho foi avaliar o comportamento de linhagens e cultivares de feijoeiro comum, tipo carioca, inoculadas com estirpes de Rizhobium tropici.CONAFE