Equisetum arvense: avaliação das possibilidades de aplicação na regeneração óssea

A procura de produtos naturais para o tratamento de doenças específicas tem aumentado muitos nos últimos anos, nomeadamente aquelas direccionadas para doenças do tecido ósseo como por exemplo a osteoporose. A utilização de fitoestrogéneos, dehydroepiandroesterona (DHEA) e vitamina K2 não só é segura como tem havido resultados clínicos preliminares estatisticamente controlados que suportam a vantagem da utilização de produtos naturais no tratamento da osteoporose. Há vários produtos de medicina tradicional Chinesa utilizados em ortopedia alegadamente com resultados satisfatórios. Neste trabalho extractos de Equisetum arvense, uma planta que cresce em solo português, foram estudados de forma a avaliar a possibilidade de serem usados como factores indutores de crescimento ósseo. Consumers are increasingly looking to natural health products to manage specific diseases like bone tissue diseases such as osteoporosis. The safety of phytoestrogens, dehydroepiandrosterone (DHEA) and vitamin K2 and the preliminary positive results from randomized controlled trials, provides some support for the use of Natural Health Products (NHP) in the prevention of osteoporosis. There are several traditional Chinese medicines commonly used to manage disorders of orthopaedics and were alleged to have obtained satisfactory results. Equisetum arvense a plant present in Potuguese soil was studied in order to evaluate the possibility of being used as an osteogenesis stimulator factor

Efeito da doxiciclina e da minociclina em células osteoblásticas humanas: estudos in vitro

A doxiciclina e a minociclina são antibióticos utilizados para eliminar infecções que surgem após o processo cirúrgico e que parecem exercer uma acção benéfica sobre o metabolismo ósseo. Os resultados obtidos neste estudo mostraram que baixas concentrações destes fármacos(1-5 μg.ml-1) estimularam a proliferação das células de medula óssea humana. A exposição das células a 10 μg.ml-1 de minociclina pareceu estimular o aparecimento de uma população celular mais proliferativa mas menos diferenciada. As concentrações de 10 μg.ml-1 de doxiciclina e 25 μg.ml-1 de minociclina atrasaram a mineralização e concentrações mais elevadas destes antibióticos (25 μg.ml-1 de doxiciclina e 50 μg.ml-1 de minociclina) foram citotóxicas. Doxycycline and minocycline are antibiotics used to eliminate infections that arise after the surgical procedure that seem to have a beneficial action in bone metabolism. Results showed that low concentrations of these drugs (1-5 μg.ml-1) stimulated human bone marrow cell proliferation. The exposure of cells to 10 μg.ml-1 minocycline stimulated the emergence of a more proliferative population but less differentiated. Concentrations of 10 μg.ml-1 doxycycline and 25 μg.ml-1 minocycline delayed the mineralization and higher concentrations of these antibiotics (25 μg.ml-1 doxycycline and 50 μg.ml-1 minocycline) were cytotoxic

The effects of tigecycline on human osteoblasts in vitro

A osteomielite, infecção no tecido ósseo, é uma doença que afecta gravemente os pacientes, uma vez que destrói grandes quantidades de tecido ósseo. A tigeciclina é um antibiótico recentemente disponível, que pode proporcionar melhores resultados no combate a infecções ósseas por organismos resistentes a outros antibióticos. Não existem na bibliografia dados disponíveis sobre a interacção da tigeciclina com as células ósseas. Neste trabalho foi demonstrado que a tigeciclina em elevadas concentrações tem efeitos nocivos para o tecido ósseo nomeadamente na proliferação dos osteoblastos. Esta informação deve ser ponderada na aplicação de dispositivos de aplicação local do antibiótico. Em baixas concentrações este fenómeno não foi verificado. Osteomyelitis (bone tissue infection), is a very serious disease affecting many patients by destroying large portions of bone tissue. Tigecycline is a recently available antibiotic to overcome bacteria resistance phenomena in bone tissue infections. No information is available regarding its direct effects on bone cells. We have demonstrated deleterious effects of high tigecycline concentrations on viability and proliferation of osteoblasts in vitro. This finding should be carefully taken into account when a local antibiotic application is used. However, at lower concentrations tigecycline this effect was not observed

Efeito da doxiciclina e da minociclina em células osteoblásticas humanas: estudos in vitro

A doxiciclina e a minociclina são antibióticos utilizados para eliminar infecções que surgem após o processo cirúrgico e que parecem exercer uma acção benéfica sobre o metabolismo ósseo. Os resultados obtidos neste estudo mostraram que baixas concentrações destes fármacos(1-5 μg.ml-1) estimularam a proliferação das células de medula óssea humana. A exposição das células a 10 μg.ml-1 de minociclina pareceu estimular o aparecimento de uma população celular mais proliferativa mas menos diferenciada. As concentrações de 10 μg.ml-1 de doxiciclina e 25 μg.ml-1 de minociclina atrasaram a mineralização e concentrações mais elevadas destes antibióticos (25 μg.ml-1 de doxiciclina e 50 μg.ml-1 de minociclina) foram citotóxicas. Doxycycline and minocycline are antibiotics used to eliminate infections that arise after the surgical procedure that seem to have a beneficial action in bone metabolism. Results showed that low concentrations of these drugs (1-5 μg.ml-1) stimulated human bone marrow cell proliferation. The exposure of cells to 10 μg.ml-1 minocycline stimulated the emergence of a more proliferative population but less differentiated. Concentrations of 10 μg.ml-1 doxycycline and 25 μg.ml-1 minocycline delayed the mineralization and higher concentrations of these antibiotics (25 μg.ml-1 doxycycline and 50 μg.ml-1 minocycline) were cytotoxic

Nasal carriage of Staphylococcus aureus among nursing students during curricular internship

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Prevalence of antibiotic (ß-lactams, tetracycline, metronidazole, erythromycin) resistance genes in periodontic infections

Objective: Porphyromonas gingivalis and Prevotella intermedia are thought to be pathogens in adult periodontitis. Antibiotherapy is usually needed in the treatment of periodontitis being often prescribed empirically. To allow prescription of a specific antibiotic treatment, identification of resistance genes should be performed. The aim of this study was the identification of the presence of TetM, TetQ, TEM, cfxA, MefA, ErmB and Nim resistance genes in previously identified P. intermedia and P. gingivalis isolated from samples collected from periodontal infections. Method: PCR was used for the identification of TetM, TetQ, TEM, cfxA, MefA, ErmB and Nim resistance genes in strains isolated from samples collected from periodontal infections. Results: It was seen that 8% of isolates had one of the tested tetracycline resistance genes. A total of 32% of β-lactamases resistance genes was observed in isolated strains. It was also observed that 2% of isolates had one of the analysed erythromycin resistance genes. None of the isolates showed the presence of the metronidazole resistance gene. Conclusions: Most strains harboring β-lactamase resistance genes had been previously identified as P. intermedia. No tetracycline resistance gene and a very low percentage of β-lactamase resistance genes were observed in P. gingivalis strains.info:eu-repo/semantics/publishedVersio

Prevalence of antibiotic (ß-lactams, tetracycline, metronidazole, erythromycin) resistance genes in periodontic infections

Objective: Porphyromonas gingivalis and Prevotella intermedia are thought to be pathogens in adult periodontitis. Antibiotherapy is usually needed in the treatment of periodontitis being often prescribed empirically. To allow prescription of a specific antibiotic treatment, identification of resistance genes should be performed. The aim of this study was the identification of the presence of TetM, TetQ, TEM, cfxA, MefA, ErmB and Nim resistance genes in previously identified P. intermedia and P. gingivalis isolated from samples collected from periodontal infections. Method: PCR was used for the identification of TetM, TetQ, TEM, cfxA, MefA, ErmB and Nim resistance genes in strains isolated from samples collected from periodontal infections. Results: It was seen that 8% of isolates had one of the tested tetracycline resistance genes. A total of 32% of β-lactamases resistance genes was observed in isolated strains. It was also observed that 2% of isolates had one of the analysed erythromycin resistance genes. None of the isolates showed the presence of the metronidazole resistance gene. Conclusions: Most strains harboring β-lactamase resistance genes had been previously identified as P. intermedia. No tetracycline resistance gene and a very low percentage of β-lactamase resistance genes were observed in P. gingivalis strains.info:eu-repo/semantics/publishedVersio

Putting people first: a multidimensional approach to health socioeconomic determinants

Person Centered Healthcare aims for the involvement of patients to provide quality healthcare services based on committed healthcare professionals. This paradigm implies shared decision-making between healthcare professionals and patients involving various aspects of the patient-healthcare relation. The present work focuses on the importance of analysing healthcare services distribution considering communities’ specificities. To develop appropriate healthcare solutions that fit people needs, public health policies should be designed in a manner that involves health stakeholders, experts and the civil society. The United Nations 2030 Agenda for Sustainable Development proposes the Sustainable Development Goal 3 in order to “ensure healthy lives and promote well-being for all at all ages” which is in alignment with the Person Centered Healthcare aims. The present work proposes a tailored made index SEHVI – Socioeconomic Health Vulnerability Index – applied to Portuguese mainland population. In the scope of the principle of patient-centered healthcare services, SEHVI aggregates seven health outcomes indicators – mortality variables – and twenty-eight health determinants indicators: healthcare resources, social protection, education, water and sanitation, employment and income, air pollution, waste, land use, housing, social participation and safety variables. Data was collected from official statistical databases – INE, PORDATA and APA – and disaggregated at the municipal scale, allowing a diagnose of people’s needs and specificities at a local level. Years 2009, 2015 and 2018 were chosen to evaluate population health status. The country national score was used as the benchmark enabling the identification of vulnerable communities. The majority (72 %) of the mainland population experiences more vulnerable health conditions than the country’s average. SEHVI scores reveal a deterioration of health determinants in the period of study. Populations’ socioeconomic and environmental conditions play an important role in health outcomes, stressing the need to provide adequate healthcare services in the context of a centered healthcare approach.info:eu-repo/semantics/publishedVersio

Measuring health vulnerability: an interdisciplinary indicator applied to Mainland Portugal

Health promotion and inequality reduction are specific goals of the United Nations 2030 Agenda, which are interconnected with several dimensions of life. This work proposes a composite index SEHVI—socioeconomic health vulnerability index—to address Portuguese population socioeconomic determinants that affect health outcomes. Variables composing SEHVI are aligned with the sustainable development goals considering data and times series availability to enable progress monitoring, and variables adequacy to translate populations’ life conditions affecting health outcomes. Data for 35 variables and three periods were collected from official national databases. All variables are part of one of the groups: Health determinants (social, economic, cultural, and environmental factors) and health outcomes (mortality indicators). Variables were standardized and normalized by “Distance to a reference” method and then aggregated into the SEHVI formula. Several statistical procedures for validation of SEHVI revealed the internal consistency of the index. For all municipalities, SEHVI was calculated and cartographically represented. Results were analyzed by statistical tests and compared for three years and territory typologies. SEHVI differences were found as a function of population density, suggesting inequalities of communities’ life conditions and in vulnerability to health.info:eu-repo/semantics/publishedVersio

Identification of TEM, CfxA, TetM and TetQ genes in periodontic infections

info:eu-repo/semantics/publishedVersio