Conferring Antioxidant Activity to an Antibacterial and Bioactive Titanium Surface through the Grafting of a Natural Extract

The main unmet medical need of bone implants is multifunctional activity, including their ability to induce rapid and physiological osseointegration, counteract bacterial biofilm formation, and prevent in situ chronic inflammation at the same time. This research starts from an already developed c.p. titanium surface with proven bioactive (in vitro hydroxyl apatite precipitation) and antibacterial activities, due to a calcium titanate layer with nano- and micro-scale roughness and loaded with iodine ions. Here, antioxidant ability was added to prevent chronic inflammation by grafting polyphenols of a green tea extract onto the surface, without compromising the other functionalities of the surface. The surface was characterized before and after functionalization through XPS analysis, zeta potential titrations, ion release measurements, in vitro bioactivity tests, SEM and fluorescence microscopy, and Folin–Ciocalteu and biological tests. The presence of grafted polyphenols as a homogeneous layer was proven. The grafted polyphenols maintained their antioxidant ability and were anchored to the surface through the linking action of Ca2+ ions added to the functionalizing solution. Iodine ion release, cytocompatibility towards human mesenchymal stem cells (hMSC), and antibacterial activity were maintained even after functionalization. The antioxidant ability of the functionalized surface was effective in preserving hMSC viability in a chemically induced pro-inflammatory environment, thus showing a scavenger activity towards toxic active species responsible for inflammation

New Magnetic Graphitized Carbon Black TiO2 Composite for Phosphopeptide Selective Enrichment in Shotgun Phosphoproteomics

Graphitized carbon black (GCB) has been employed for extraction of several classes of analytes, due to the large surface area and the unique chemistry of its surface groups that allows for extracting a wide range of analytes, including polar, acidic compounds. Despite the fact that structurally related materials, such as graphene, found application as hybrid-components in phosphoproteomics, surprisingly, GCB has never been used for the selective enrichment of phosphopeptides. For this purpose, in the present work we used GCB to prepare a magnetic composite with TiO2 (mGCB@TiO2) that was then applied to yeast total extracts. We exploited the high surface area provided by nanostructures, the presence of nano-TiO2 for selective binding of phosphopeptides, and the magnetic responsiveness of magnetite for solid-phase separation. The material was extensively characterized at each modification step by transmission electron microscopy, Fourier-transformed infrared spectroscopy, thermogravimetric analysis, Raman spectroscopy, and porosimetry. Next, the new system was applied for the enrichment of casein phosphopeptides from a simulated tryptic digest with bovine serum albumin (BSA:casein, 100:1). Finally, after assessing the potential applicability, the composite was employed for enriching phosphopeptides from yeast protein digests. This allowed us not only to optimize the enrichment protocol but also to fully compare its performance to commercial TiO2 spin columns. To achieve this aim, the optimized enrichment protocol was included in a typical shotgun proteomics analytical workflow based on nanoHPLC-MS/MS analysis

Discovery of the first potent and selective Mycobacterium tuberculosis Zmp1 inhibitor

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Semaphorin 4D regulates gonadotropin hormone–releasing hormone-1 neuronal migration through PlexinB1–Met complex

In mammals, reproduction is dependent on specific neurons secreting the neuropeptide gonadotropin hormone–releasing hormone-1 (GnRH-1). These cells originate during embryonic development in the olfactory placode and migrate into the forebrain, where they become integral members of the hypothalamic–pituitary–gonadal axis. This migratory process is regulated by a wide range of guidance cues, which allow GnRH-1 cells to travel over long distances to reach their appropriate destinations. The Semaphorin4D (Sema4D) receptor, PlexinB1, is highly expressed in the developing olfactory placode, but its function in this context is still unknown. Here, we demonstrate that PlexinB1-deficient mice exhibit a migratory defect of GnRH-1 neurons, resulting in reduction of this cell population in the adult brain. Moreover, Sema4D promotes directional migration in GnRH-1 cells by coupling PlexinB1 with activation of the Met tyrosine kinase (hepatocyte growth factor receptor). This work identifies a function for PlexinB1 during brain development and provides evidence that Sema4D controls migration of GnRH-1 neurons

Surface functionalization of Ti6Al4V-ELI alloy with antimicrobial peptide nisin

Implant-associated infections are a severe global concern, especially in the case of orthopedic implants intended for long-term or permanent use. The traditional treatment through systemic antibiotic administration is often inefficient due to biofilm formation, and concerns regarding the development of highly resistant bacteria. Therefore, there is an unfulfilled need for antibiotic-free alternatives that could simultaneously support bone regeneration and prevent bacterial infection. This study aimed to perform, optimize, and characterize the surface functionalization of Ti6Al4V-ELI discs by an FDA-approved antimicrobial peptide, nisin, known to hold a broad antibacterial spectrum. Accordingly, nisin bioactivity was also evaluated by in vitro release tests both in physiological and inflammatory pH conditions. Several methods, such as X-ray photoelectron spectroscopy (XPS), and Kelvin Probe atomic force microscopy confirmed the presence of a physisorbed nisin layer on the alloy surface. The functionalization performed at pH 6–7 was found to be especially effective due to the nisin configuration exposing its hydrophobic tail outwards, which is also responsible for its antimicrobial action. In addition, the first evidence of gradual nisin release both in physiological and inflammatory conditions was obtained: the static contact angle becomes half of the starting one after 7 days of soaking on the functionalized sample, while it becomes 0◦ on the control samples. Finally, the evaluation of the antibacterial performance toward the pathogen Staphylococcus aureus after 24 h of inoculation showed the ability of nisin adsorbed at pH 6 to prevent bacterial microfouling into biofilm-like aggregates in comparison with the uncoated specimens: viable bacterial colonies showed a reduction of about 40% with respect to the un-functionalized surface and the formation of microcolonies (biofilm-like aggregates) is strongly affected

Wappfruit: a project for the optimisation of water use in agriculture

The WAPPFRUIT project is related to the optimisation of irrigation techniques in the Piemonte Region, Northwest Italy. The main goal is to control irrigation to understand if it is possible to reduce the volume of water used for irrigation and also save energy. The project involves several stakeholders, among which Politecnico and the University of Torino, Piemonte Region, Agrion Foundation for research in agriculture, and three farms (two apple orchards and one Actinidia orchard). The optimisation relies on soil matric potential measurements at several soil depths. The irrigation will be triggered using a particular algorithm which is based on a system of matric potential thresholds at the depths of 20 and 40 cm. These thresholds are based on soil texture, and vegetation species (including root depth). Each orchard is divided into two parts: an “experimental area” where the irrigation algorithm will be tested, and an area that will be irrigated as usual by farmers. Each orchard is equipped with four to six measurement nodes, with soil water content and soil matric potential profile having measures at 20, 40, and 60 cm of depth. The retention curves, as well as the spatial and temporal variability of soil water content and soil matric potential, can be inferred from measures, which reveal high volumes of water used for irrigation (frequently the soil was near saturation conditions). In addition, all the soils show, in the retention curves, a hysteresis due to wetting/drying cycles. The farmers continued to irrigate as usual in the two parts of the fields up to October 2022. Hence, to investigate the matric potential behavior and identify good estimates of thresholds, modeling approaches are important for the simulation of soil without irrigation, to understand when water stress conditions could occur. To this purpose, two models are used to simulate the water fluxes in the atmosphere and the soil (and, particularly, the matric potential). The two models adopted are the hydrological model Hydrus 1D and the land-surface model CLM5. Forcing the models with the precipitation summed to irrigation of the fields, Hydrus, in its 1D formulation did not yield reliable results, although more studies are needed to fully understand the causes for the misrepresentation. The CLM model yields instead more reliable outcomes. The CLM model is then used to simulate the behavior of the soil matric potential under the hypothesis of no irrigation. The results illustrate that the matric potential threshold for triggering irrigation could be around -50 kPa at 20 cm, whereas the threshold at 40 cm for the deactivation of irrigation could be around -40 kPa for the sites with apple orchards. The site with Actinidia could have the aforementioned thresholds equal to -40 kPa at 20 cm and -30 kPa at 40 cm

Surface modified Ti6Al4V for enhanced bone bonding ability - effects of silver and corrosivity at simulated physiological conditions from a corrosion and metal release perspective

Abstract Different surface treatments, with and without silver (Ag), of a Ti6Al4V alloy for increased bone bonding ability were investigated and compared with non-treated surfaces. Studies were conducted at 37 °C in phosphate buffered saline (PBS, pH 7.4) of varying hydrogen peroxide (H2O2) and bovine serum albumin (BSA) concentrations. Increased levels of metal release and corrosion were observed in the presence of both H2O2 and BSA due complexation with Ti and Al in the surface oxide, respectively. Ag release was enhanced by the presence of BSA. Galvanic effects by Ag were minor, but possibly observed in the most corrosive environment