4 research outputs found
Dendrimersomes: a new vesicular nano-platform for MR-molecular imaging applications
A new class of nanovesicles formed by the self-assembly of amphiphilic Janus dendrimers, dendrimersomes, loaded with hydrophilic or amphiphilic magnetic resonance imaging chelates shows promising properties as a novel, efficient and versatile nano platform for biomedical imaging
Π’Π΅ΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΡΠ΅Π½ΠΊΠ° ΡΠ°Π·ΡΠ΅ΡΠ°ΡΡΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΡΠ°ΡΡΠΌΠ°Π½Π° Π² ΡΠΈΡΡΠ΅ΠΌΠ°Ρ ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ²Π΅ΡΠ°, ΠΏΡΠΈΠΌΠ΅Π½ΡΠ΅ΠΌΡΡ Π² Π°Π²ΠΈΠΎΠ½ΠΈΠΊΠ΅
Π Π°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°Π΅ΡΡΡ Π·Π°Π΄Π°ΡΠ° ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠ°Π·ΡΠ΅ΡΠ°ΡΡΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΌΠ°ΡΠ΅ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΡΠ°ΡΡΠΌΠ°Π½Π°, ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠ΅Π³ΠΎ ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΡ ΡΠ²Π΅ΡΠ½ΠΎΡΡΠΈ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΈΡΡΠ΅ΠΌΠ°Ρ
ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½ΠΈΡ: RGB ΠΈ XY. ΠΡΠΈΠ²ΠΎΠ΄ΡΡΡΡ ΠΏΡΠΈΠΌΠ΅ΡΡ Π³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΈΠΉ ΡΠ²Π΅ΡΠΎΠ² ΠΈ ΠΎΡΡΠ΅Π½ΠΊΠΎΠ² Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΈΡΡΠ΅ΠΌΠ°Ρ
ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ Π²ΡΠ²ΠΎΠ΄ ΡΠΎΡΠΌΡΠ»Ρ Π΄Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠ°Π·ΡΠ΅ΡΠ°ΡΡΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΡΠ°ΡΡΠΌΠ°Π½Π°, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠ΅ΠΉ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ³ΠΎ (Π½Π΅ Π½ΡΠ»Π΅Π²ΠΎΠ³ΠΎ) ΠΌΠΎΠ΄ΡΠ»Ρ Π΄ΠΈΡΠΊΡΠ΅ΡΠ° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ (x,y)-ΠΊΠΎΠΎΡΠ΄ΠΈΠ½Π°Ρ ΡΠ²Π΅ΡΠ½ΠΎΡΡΠΈ ΠΏΠΎ ΠΎΡΡΠΌ XY ΠΏΡΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΊΠΎΠ΄Π° RGB Π½Π° ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ»ΡΠ½ΡΡ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΏΠΎ Π»ΡΠ±ΠΎΠΌΡ ΠΈΠ· ΡΡΠ΅Ρ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² R, G, B. Π Π°ΡΡΡΠΈΡΠ°Π½Ρ ΡΠΈΡΠ»ΠΎΠ²ΡΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΡ ΡΠ°Π·ΡΠ΅ΡΠ°ΡΡΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ Π·Π½Π°ΡΠ΅Π½ΠΈΡ Π½Π°ΡΠ°Π»ΡΠ½ΡΡ
ΠΈ ΠΊΠΎΠ½Π΅ΡΠ½ΡΡ
ΠΊΠΎΠ΄ΠΎΠ² RGB, ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΡΡ
Π΄ΠΎΡΡΠΈΠ³Π°Π΅ΡΡΡ ΠΌΠΈΠ½ΠΈΠΌΡΠΌ ΠΏΡΠΈΡΠ°ΡΠ΅Π½ΠΈΠΉ DΡ
, Dy Π΄Π»Ρ Π΅Π΄ΠΈΠ½ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΡΠ°ΡΠ΅Π½ΠΈΡ ΠΊΠΎΠ΄Π° RGB ΠΈ Π²ΠΎΡΡΠΌΠΈΠ±ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ΄ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° R, G, B
Aerobic exercise training enhances the in vivo cholesterol trafficking from macrophages to the liver independently of changes in the expression of genes involved in lipid flux in macrophages and aorta
Abstract\ud
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Background\ud
Regular exercise prevents and regresses atherosclerosis by improving lipid metabolism and antioxidant defenses. Exercise ameliorates the reverse cholesterol transport (RCT), an antiatherogenic system that drives cholesterol from arterial macrophages to the liver for excretion into bile and feces. In this study we analyzed the role of aerobic exercise on the in vivo RCT and expression of genes and proteins involved in lipid flux and inflammation in peritoneal macrophages, aortic arch and liver from wild type mice.\ud
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Methods\ud
Twelve-week-old male mice were divided into sedentary and trained groups. Exercise training was performed in a treadmill (15Β m/min, 30Β min/day, 5Β days/week). Plasma lipids were determined by enzymatic methods and lipoprotein profile by fast protein liquid chromatography. After intraperitoneal injection of J774-macrophages the RCT was assessed by measuring the recovery of 3H-cholesterol in plasma, feces and liver. The expression of liver receptors was determined by immunoblot, macrophages and aortic mRNAs by qRT-PCR. 14C-cholesterol efflux mediated by apo A-I and HDL2 and the uptake of 3H-cholesteryl oleoyl ether (3H-COE)-acetylated-LDL were determined in macrophages isolated from sedentary and trained animals 48Β h after the last exercise session.\ud
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Results\ud
Body weight, plasma lipids, lipoprotein profile, glucose and blood pressure were not modified by exercise training. A greater amount of 3H-cholesterol was recovered in plasma (24Β h and 48Β h) and liver (48Β h) from trained animals in comparison to sedentary. No difference was found in 3H-cholesterol excreted in feces between trained and sedentary mice. The hepatic expression of scavenger receptor class B type I (SR-BI) and LDL receptor (B-E) was enhanced by exercise. We observed 2.8 and 1.7 fold rise, respectively, in LXR and Cyp7a mRNA in the liver of trained as compared to sedentary mice. Macrophage and aortic expression of genes involved in lipid efflux was not systematically changed by physical exercise. In agreement, 14C-cholestrol efflux and uptake of 3H-COE-acetylated-LDL by macrophages was similar between sedentary and trained animals.\ud
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Conclusion\ud
Aerobic exercise in vivo accelerates the traffic of cholesterol from macrophages to the liver contributing to prevention and regression of atherosclerosis, independently of changes in macrophage and aorta gene expression.Fundação de Amparo à Pesquisa do Estado de Sao Paulo - FAPESP 12/04831-1 to MP, UFM and MLCCG; FAPESP 07/50387-8 to MP, 2011/15153-1 to PR, 06/52702-5 to DDFMRocco, 13/02854-7 to LS\ud
Okuda, 12/19112-0 to AML, 10/50108-4 to GC, 12/18724-2 to KS, 11/04631-0 to DJG, 09/53412-9 to RS Pinto; 12/12088-7 to RTI, 14/07155 to GFConselho Nacional de Desenvolvimento CientΓfico e TecnolΓ³gico (158314/\ud
2014-0 to DJG