¿Cual es la función de las nucleoporinas AtSAR1 y AtSAR3 en la meiosis de " Arabidopsis thaliana"

El complejo del poro nuclear regula el transporte núcleo-citoplasmático. Existe una relación con la supresión de la resistencia auxinas cuando se alteran algunas nucleoporinas. Concretamente, mutaciones en AtSAR1/AtNUP160 (Suppressor of Auxin Resistance1) y AtSAR3/AtNUP96 suprimen el fenotipo característico de los mutantes Ataxr1 (Auxin Resistant1) como: pérdida de la dominancia apical y del gravitropismo de la raíz, reducción de la formación de raíces laterales, aumento de la longitud de la raíz primaria, reducción de la altura de la planta y de la fertilidad. AtAXR1 es una proteína implicada en la ruta de rubilación. Esta proteína es determinante en la degradación de la proteína Aux/IAA, represora de la expresión de genes de respuesta a auxinas. Además se ha demostrado fundamental durante el proceso de localización de los sobrecruzamientos en la meiosis de Arabidopsis. Los mutantes para las proteínas AtSAR presentaron un fenotipo pleiotrópico, manifestando diversos defectos en el desarrollo vegetativo y la fertilidad. El objetivo del presente trabajo fue caracterizar los mutantes Atsar1-4 y Atsar3-3 para determinar si las alteraciones en fertilidad son debidas a anomalías durante la división meiótica y conocer más sobre las funciones del complejo del poro nuclear

Synthetically induced Arabidopsis thaliana autotetraploids provide insights into the analysis of meiotic mutants with altered crossover frequency

Mutations affecting crossover (CO) frequency and distribution lead to the presence of univalents during meiosis, giving rise to aneuploid gametes and sterility. These mutations may have a different effect after chromosome doubling. The combination of altered ploidy and mutations could be potentially useful to gain new insights into the mechanisms and regulation of meiotic recombination; however, studies using autopolyploid meiotic mutants are scarce. Here, we have analyzed the cytogenetic consequences in colchicine-induced autotetraploids (colchiploids) from different Arabidopsis mutants with an altered CO frequency. We have found that there are three types of mutants: mutants in which chiasma frequency is doubled after chromosome duplication (zip4, mus81), as in the control; mutants in which polyploidy leads to a higher-than-expected increase in chiasma frequency (asy1, mer3, hei10, and mlh3); and mutants in which the rise in chiasma frequency produced by the presence of two extrachromosomal sets is less than doubled (msh5, fancm). In addition, the proportion of class I/class II COs varies after chromosome duplication in the control. The results obtained reveal the potential of colchiploid meiotic mutants for better understanding of the function of key proteins during plant meiosis. This is especially relevant considering that most crops are polyploids.Depto. de Genética, Fisiología y MicrobiologíaFac. de Ciencias BiológicasTRUEpu

Augmented Reality in the Formation of Minimal Incision Podiatric Surgery

BACKGROUND: This project shows that the use of augmented reality (AR) technology in the study of podiatric surgery has a positive impact on student outcomes. AR technology can help to enrich the information that provides elements such as X-rays or other diagnostic tools. METHODS : A didactic material with augmented reality was created through the use of markers for the course subjects of Surgical Techniques II, e.g., the topic of hallux valgus surgery, and was compared with the didactic material for 5th metatarsal surgery using PowerPoint and video. The comparison was assessed by a validated questionnaire after providing 2 hours of teaching for each of the subjects to 80 students in a master of surgery program during the 2013-2017 academic years. RESULTS: Analysis of the components of the questionnaire showed that component 1. training, attention and motivation; component 2. freelance work; and component 4. 3D compression were statistically significant at p < 0.05. However, component 3, which compared the technologies used with cadaveric material, was not statistically significant regarding any of its items. CONCLUSION: The current study shows that using augmented reality technology for the study of minimally invasive surgery of the foot increases the attention, the motivation and therefore the learning of the students, in addition to providing three-dimensional images of the surgical movements that are more accurate regarding reality.PreprintPodologí

Structural Maintenance of Chromosomes 5/6 Complex Is Necessary for Tetraploid Genome Stability in Arabidopsis thaliana

Polyploidization is a common phenomenon in the evolution of flowering plants. However, only a few genes controlling polyploid genome stability, fitness, and reproductive success are known. Here, we studied the effects of loss-of-function mutations in NSE2 and NSE4A subunits of the Structural Maintenance of Chromosomes 5/6 (SMC5/6) complex in autotetraploid Arabidopsis thaliana plants. The diploid nse2 and nse4a plants show partially reduced fertility and produce about 10% triploid offspring with two paternal and one maternal genome copies. In contrast, the autotetraploid nse2 and nse4a plants were almost sterile and produced hexaploid and aneuploid progeny with the extra genome copies or chromosomes coming from both parents. In addition, tetraploid mutants had more severe meiotic defects, possibly due to the presence of four homologous chromosomes instead of two. Overall, our study suggests that the SMC5/6 complex is an important player in the maintenance of tetraploid genome stability and that autotetraploid Arabidopsis plants have a generally higher frequency of but also higher tolerance for aneuploidy compared to diploids

Distance learning ects and flipped classroom in the anatomy learning: comparative study of the use of augmented reality, video and notes

Background: The establishment of the ECTS (European Credit Transfer System) is one of the pillars of the European Space of Higher Education. This way of accounting for the time spent in training has two essential parts, classroom teaching (work with the professor) and distance learning (work without the professor, whether in an individual or collective way). Much has been published on the distance learning part, but less on the classroom teaching section. In this work, the authors investigate didactic strategies and associated aids for distance learning work in a concept based on flipped classroom where transmitting information is carried out with aids that the professor prepares, so that the student works in an independent way before the classes, thus being able to dedicate the classroom teaching time to more complex learning and being able to count on the professor’s help. Methods: Three teaching aids applied to the study of anatomy have been compared: Notes with images, videos, and augmented reality. Four dimensions have been compared: the time spent, the acquired learnings, the metacognitive perception, and the prospects of the use of augmented reality for study. Results: The results show the effectiveness, in all aspects, of augmented reality when compared with the rest of aids. The questionnaire assessed the acquired knowledge through a course exam, where 5.60 points were obtained for the notes group, 6.54 for the video group, and 7.19 for the augmented reality group. That is 0.94 more points for the video group compared with the notes and 1.59 more points for the augmented reality group compared with the notes group. Conclusions: This research demonstrates that, although technology has not been sufficiently developed for education, it is expected that it can be improved in both the autonomous work of the student and the academic training of health science students and that we can teach how to learn. Moreover, one can see how the grades of the students who studied with augmented reality are more grouped and that there is less dispersion in the marks compared with other materials.Financial support of the Foundation “Prevere Bernat Beny”.Pedagogí

Duplication and divergence: new insights into AXR1 and AXL functions in DNA repair and meiosis

Rubylation is a conserved regulatory pathway similar to ubiquitination and essential in the response to the plant hormone auxin. In Arabidopsis thaliana, AUXIN RESISTANT1 (AXR1) functions as the E1-ligase in the rubylation pathway. The gene AXR1-LIKE (AXL), generated by a relatively recent duplication event, can partially replace AXR1 in this pathway. We have analysed mutants defcient for both proteins and complementation lines (with the AXR1 promoter and either AXR1 or AXL coding sequences) to further study the extent of functional redundancy between both genes regarding two processes: meiosis and DNA repair. Here we report that whereas AXR1 is essential to ensure the obligatory chiasma, AXL seems to be dispensable during meiosis, although its absence slightly alters chiasma distribution. In addition, expression of key DNA repair and meiotic genes is altered when either AXR1 or AXL are absent. Furthermore, our results support a signifcant role for both genes in DNA repair that was not previously described. These fndings highlight that AXR1 and AXL show a functional divergence in relation to their involvement in homologous recombination, exemplifying a duplicate retention model in which one copy tends to have more sub-functions than its paralog

Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis.

The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1 The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants

Managing by processes system to evaluate the environmental performance from the implementation of PDCA diagnosis

RESUMEN: Este texto evidencia el diseño de un sistema de gestión por procesos para la evaluación del desempeño ambiental. Objetivo. Diagnosticar el estado actual ambiental de los procesos que hacen parte de las organizaciones, identificando sus aspectos y valorando los impactos ambientales que se puedan generar teniendo en cuenta las actividades que se realizan en la empresa. Materiales y métodos. Identificar los procesos más importantes mediante visitas realizadas por los investigadores del proyecto a la empresa en estudio. Resultados. Se determinaron los procesos más importantes de la organización sobre los cuales se desarrolló la investigación. Conclusión. La práctica de la gestión ambiental de la empresa estudiada es insuficiente.ABSTARCT: This paper introduces a managing by processes system to evaluate the environmental performance. Objective. To diagnose the current environmental status of the processes that comprise organizations, identifying their aspects and assessing the environmental impacts that can be generated, taking into account the activities developed in the

mHealth intervention to improve quality of life in patients with chronic diseases during the COVID-19 crisis in Paraguay: A study protocol for a randomized controlled trial

Background Patients with chronic disease represent an at-risk group in the face of the COVID-19 crisis as they need to regularly monitor their lifestyle and emotional management. Coping with the illness becomes a challenge due to supply problems and lack of access to health care facilities. It is expected these limitations, along with lockdown and social distancing measures, have affected the routine disease management of these patients, being more pronounced in low- and middle-income countries with a flawed health care system. Objectives The purpose of this study is to describe a protocol for a randomized controlled trial to test the efficacy of the Adhera® MejoraCare Digital Program, an mHealth intervention aimed at improving the quality of life of patients with chronic diseases during the COVID-19 outbreak in Paraguay. Method A two-arm randomized controlled trial will be carried out, with repeated measures (baseline, 1-month, 3-month, 6-month, and 12-month) under two conditions: Adhera® MejoraCare Digital Program or waiting list. The primary outcome is a change in the quality of life on the EuroQol 5-Dimensions 3-Levels Questionnaire (EQ-5D-3L). Other secondary outcomes, as the effect on anxiety and health empowerment, will be considered. All participants must be 18 years of age or older and meet the criteria for chronic disease. A total of 96 participants will be recruited (48 per arm). Conclusions It is expected that the Adhera® MejoraCare Digital Program will show significant improvements in quality of life and emotional distress compared to the waiting list condition. Additionally, it is hypothesized that this intervention will be positively evaluated by the participants in terms of usability and satisfaction. The findings will provide new insights into the viability and efficacy of mHealth solutions for chronic disease management in developing countries and in times of pandemic