Estudio de la formación de cristales por bacterias en medios tamponados

Se investiga la precipitación bacteriana de carbonato cálcico en el medio B-4 tamponado con tres sistemas bufer diferentes a los pH 7 Y 8, por 96 cepas bacterianas aisladas del suelo. A pH 8 se observa precipitación de C03Ca en todas las cepas ensayadas, mientras que el pH 7 solo ocurre excepcionalmente. Los tampones tris-hidroxmetil amino metano-HCl y clorhidrato de trietanol amina-OHNa se revelaron idóneos para estas experiencias, mientras que el tampón 5:5 dietil barbiturato sódico-HCl no resultó apropiado.Calcium carbonate precipitation by, 96 strains isolated from soil, was studied in B-4 buffered medium using three different systems at pH 7 and 8. CaC03 precipitation at pH 8 is observed in aH the strains investigated, whereas it occurs only exceptionally at pH 7. The tris-hydroximethyl amino methane-CIH and hydrochloride triethanol amine-NaOH were adequated for these experiments, but the 5:5 diethyl sodium barbiturate was not considered suitable

Estudio de la formación de cristales por bacterias en medios tamponados

Calcium carbonate precipitation by, 96 strains isolated from soil, was studied in B-4 buffered medium using three different systems at pH 7 and 8. CaC03 precipitation at pH 8 is observed in aH the strains investigated, whereas it occurs only exceptionally at pH 7. The tris-hydroximethyl amino methane-CIH and hydrochloride triethanol amine-NaOH were adequated for these experiments, but the 5:5 diethyl sodium barbiturate was not considered suitable.Se investiga la precipitación bacteriana de carbonato cálcico en el medio B-4 tamponado con tres sistemas bufer diferentes a los pH 7 Y 8, por 96 cepas bacterianas aisladas del suelo. A pH 8 se observa precipitación de C03Ca en todas las cepas ensayadas, mientras que el pH 7 solo ocurre excepcionalmente. Los tampones tris-hidroxmetil amino metano-HCl y clorhidrato de trietanol amina-OHNa se revelaron idóneos para estas experiencias, mientras que el tampón 5:5 dietil barbiturato sódico-HCl no resultó apropiado

Influencia el pH en la formación del cristales de carbonato cálcico por bacterias del suelo

The effect of pH on the calcium carbonate precipitation (crystal formations) was studied with 96 strains isolated from the soil. Crystal formations in culture media were detected with initial pH between 5.5 to 9. These data show that the optimum pH to crystal formations may be well stablished during bacterial growth, and that is not necessary the previous alkalinization of the culture media to calcium carbonate precipitation.Se estudia la influencia del pH en la precipitación de carbonato cálcico por 96 cepas bacterianas aisladas del suelo. Se obtienen cristales en medios con pH iniciales comprendidos entre 5,5 y 9. No es necesaria la alcalinización previa de los medios, ya que el pH óptimo para la precipitación puede alcanzarse durante el desarrollo bacteriano

Influencia el pH en la formación del cristales de carbonato cálcico por bacterias del suelo

Se estudia la influencia del pH en la precipitación de carbonato cálcico por 96 cepas bacterianas aisladas del suelo. Se obtienen cristales en medios con pH iniciales comprendidos entre 5,5 y 9. No es necesaria la alcalinización previa de los medios, ya que el pH óptimo para la precipitación puede alcanzarse durante el desarrollo bacteriano.The eHect of pH on the calcium carbonate precipitation (crystal formations) was studied with 96 strains isolated from the soíl. Crystal formations in culture media were detected with initial pH between 5.5 to 9. These data show that the optimum pH to crystal formations may be well stablished during bacterial growth, and that is not necessary the previous alkalinization of the culture media to calcium carbonate precipitation

Patient preferences and treatment safety for uncomplicated vulvovaginal candidiasis in primary health care

<p>Abstract</p> <p>Background</p> <p>Vaginitis is a common complaint in primary care. In uncomplicated candidal vaginitis, there are no differences in effectiveness between oral or vaginal treatment. Some studies describe that the preferred treatment is the oral one, but a Cochrane's review points out inconsistencies associated with the report of the preferred way that limit the use of such data. Risk factors associated with recurrent vulvovaginal candidiasis still remain controversial.</p> <p>Methods/Design</p> <p>This work describes a protocol of a multicentric prospective observational study with one year follow up, to describe the women's reasons and preferences to choose the way of administration (oral vs topical) in the treatment of not complicated candidal vaginitis. The number of women required is 765, they are chosen by consecutive sampling. All of whom are aged 16 and over with vaginal discharge and/or vaginal pruritus, diagnosed with not complicated vulvovaginitis in Primary Care in Madrid.</p> <p>The main outcome variable is the preferences of the patients in treatment choice; secondary outcome variables are time to symptoms relief and adverse reactions and the frequency of recurrent vulvovaginitis and the risk factors. In the statistical analysis, for the main objective will be descriptive for each of the variables, bivariant analysis and multivariate analysis (logistic regression).. The dependent variable being the type of treatment chosen (oral or topical) and the independent, the variables that after bivariant analysis, have been associated to the treatment preference.</p> <p>Discussion</p> <p>Clinical decisions, recommendations, and practice guidelines must not only attend to the best available evidence, but also to the values and preferences of the informed patient.</p

Phenolic and volatile compounds in Quercus humboldtii Bonpl. wood: effect of toasting with respect to oaks traditionally used in cooperage

[Background] The most frequent renewal of barrels of different origins and species is a current trend, but this demand does not match the current availability of wood. An alternative could be the use of Quercus humboldtii wood. However, there is little information about its composition. Because of this, the aim was to study low‐molecular‐weight phenols (LMWP), ellagitannins and volatile compounds in untoasted and toasted Q. humboldtii oak, and compare these with the species traditionally used in cooperage: Q. petraea (French and Romanian) and Q. alba (American). [Results] The LMWPs in Q. humboldtii were comparable to those in Q. petraea and Q. alba. Ellagitannin composition in Q. humboldtii was similar to that in Q. alba. The toasting process improved volatile composition, mainly in Q. humboldtii, presenting the highest concentration of several volatile compounds. [Conclusion] The results indicate that Q. humboldtii could be considered suitable for aging wine, although it is necessary to continue the study of this wood species to confirm its potential use in oenologyThis work was financed by the European Regional Development Fund FEDER, Interreg Spain–Portugal Programme, under the framework of the project Iberphenol, POCTEP—Programa Operativo Cooperación Transfron, CONICYT, Fondecyt de Iniciación Grant No. 11150462, Chile, Ministerio de Economía y Competitividad, AGL2014‐ 54602P, Consejería de Educación, Junta de Castilla y León, VA028U16 and with the collaboration of Tonelería Nacional Ltda, Santiago de Chile. G.G.‐G. would like to acknowledge the financial support given by CONICYT through BCH/Doctorado (72170532)Peer reviewe

Structured intermittent interruption of chronic HIV infection treatment with highly active antiretroviral therapy: Effects on leptin and TNF-alpha.

This is a copy of an article published in the AIDS Research and Human Retroviruses © 2011 [copyright Mary Ann Liebert, Inc.]; AIDS Research and Human Retroviruses is available online at: http://online.liebertpub.com. Comparative Study; Journal Article;The changes in nutritional parameters and adipocytokines after structured intermittent interruption of highly active antiretroviral treatment of patients with chronic HIV infection are analyzed. Twenty-seven patients with chronic HIV infection (median CD4+ T cell count/microl: nadir, 394; at the beginning of structured interruptions, 1041; HIV viral load: nadir, 41,521 copies/ml; at the beginning of structured interruptions <50 copies/ml; median time of previous treatment: 60 months) were evaluated during three cycles of intermittent interruptions of therapy (8 weeks on/4 weeks off). CD4+ T cell count, HIV viral load, anthropometric measures, and serum concentrations of triglycerides, cholesterol, leptin, and tumor necrosis factor and its soluble receptors I and II were determined. After the three cycles of intermittent interruptions of therapy, no significant differences in CD4+ T cell count/microl, viral load, or serum concentrations of cholesterol or triglycerides with reference to baseline values were found. A near-significant higher fatty mass (skinfold thicknesses, at the end, 121 mm, at the beginning, 100 mm, p = 0.100), combined with a significant increase of concentration of leptin (1.5 vs. 4.7 ng/ml, p = 0,044), as well as a decrease in serum concentrations of soluble receptors of tumor necrosis factor (TNFRI, 104 vs. 73 pg/ml, p = 0.022; TNFRII 253 vs. 195 pg/ml, p = 0.098) were detected. Structured intermittent interruption of highly active antiretroviral treatment of patients with chronic HIV infection induces a valuable positive modification in markers of lipid turnover and adipose tissue mass.Ye