Unexpected homology between inducible cell wall protein QID74 of filamentous fungi and BR3 salivary protein of the insect Chironomus

A gene, qid74, of mycoparasitic filamentous fungus Trichoderma harzianum and its allies encodes a cell wall protein that is induced by replacing glucose in the culture medium with chitin (simulated mycoparasitism conditions). Because no trace of this gene can be detected in related species such as Gibberella fujikuroi and Saccharomyces cerevisiae, the qid74 gene appears to have arisen de novo within the genus Trichoderma. Qid74 protein, 687 residues long, is now seen as highly conserved tandem repeats of the 59- residue-long unit. This unit itself, however, may have arisen as tandem repeats of the shorter 13-residue-long basic unit. Within the genus Trichoderma, the amino acid sequence of Qid74 proteins has been conserved in toto. The most striking is the fact that Qid74 shares 25.3% sequence identity with the carboxyl-terminal half of the 1,572-residue-long BR3 protein of the dipteran insect Chironomus tentans. BR3 protein is secreted by the salivary gland of each aquatic larva of Chironomus to form a tube to house itself. Furthermore, the consensus sequence derived from these 59-residue-long repeating units resembles those of epidermal growth factor-like domains found in divergent invertebrate and vertebrate proteins as to the positions of critical cysteine residues and homology of residues surrounding these cysteines.Comisión Interministerial de la Ciencia y la Tecnología BIO 94-0289European Commission TS3-CT92-014

Ciliochoroidal detachment following pure sulfur hexafluoride injection in Descemet stripping automated endothelial keratoplasty

Received 29 October 2016, Accepted 6 March 2017, Available online 20 April 2017Unidad Docente de Inmunología, Oftalmología y ORLFac. de Óptica y OptometríaTRUEinpres

Purification and characterization of an endo-beta-1,6-glucanase from Trichoderma harzianum that is related to its mycoparasitism

he enzymes from Trichoderma species that degrade fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal plant pathogens. The mycoparasite Trichoderma harzianum produces at least two extracellular beta-1,6-glucanases, among other hydrolases, when it is grown on chitin as the sole carbon source. One of these extracellular enzymes was purified to homogeneity after adsorption to its substrate, pustulan, chromatofocusing, and, finally, gel filtration. The apparent molecular mass was 43,000, and the isoelectric point was 5.8. The first 15 amino acids from the N terminus of the purified protein have been sequenced. The enzyme was specific for beta-1,6 linkages and showed an endolytic mode of action on pustulan. Further characterization indicated that the enzyme by itself releases soluble sugars and produces hydrolytic halli on yeast cell walls. When combined with other T. harzianum cell wall-degrading enzymes such as beta-1,3-glucanases and chitinases, it hydrolyzes filamentous fungal cell walls. The enzyme acts cooperatively with the latter enzymes, inhibiting the growth of the fungi tested. Antibodies against the purified protein also indicated that the two identified beta-1,6-glucanases are not immunologically related and are probably encoded by two different genesEspaña CICYT BIO91-1078Comunidad Europea TS3-CT92-014

A novel endo-β-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum

The mycoparasitic fungus Trichoderma harzianum CECT 2413 produces at least three extracellular β-1,3-glucanases. The most basic of these extracellular enzymes, named BGN13.1, was expressed when either fungal cell wall polymers or autoclaved mycelia from different fungi were used as the carbon source. BGN13.1 was purified to electrophoretic homogeneity and was biochemically characterized. The enzyme was specific for β-1,3 linkages and has an endolytic mode of action. A synthetic oligonucleotide primer based on the sequence of an internal peptide was designed to clone the cDNA corresponding to BGN13.1. The deduced amino acid sequence predicted a molecular mass of 78 kDa for the mature protein. Analysis of the amino acid sequence indicates that the enzyme contains three regions, one N-terminal leader sequence; another, nondefined sequence; and one cysteine-rich C-terminal sequence. Sequence comparison shows that this β-1,3-glucanase, first described for filamentous fungi, belongs to a family different from that of its previously described bacterial, yeast, and plant counterparts. Enzymatic-activity, protein, and mRNA data indicated that bgn13.1 is repressed by glucose and induced by either fungal cell wall polymers or autoclaved yeast cells and mycelia. Finally, experimental evidence showed that the enzyme hydrolyzes yeast and fungal cell wall

A detailed chemistry model for transient hydrogen and carbon monoxide catalytic recombination on parallel flat Pt surfaces implemented in an integral code

A detailed chemistry model has been adapted and developed for surface chemistry, heat and mass transfer between H2/CO/air/steam/CO2 mixtures and vertical parallel Pt-coated surfaces. This model is based onto a simplified Deutschmann reaction scheme for methane surface combustion and the analysis by Elenbaas for buoyancy-induced heat transfer between parallel plates. Mass transfer is treated by the heat and mass transfer analogy. The proposed model is able to simulate the H2/CO recombination phenomena characteristic of parallel-plate Passive Autocatalytic Recombiners (PARs), which have been proposed and implemented as a promising hydrogen-control strategy in the safety of nuclear power stations or other industries. The transient model is able to approach the warm-up phase of the PAR and its shut-down as well as the dynamic changes within the surrounding atmosphere. The model has been implemented within the MELCOR code and assessed against results of the Battelle Model Containment tests of the Zx series. Results show accurate predictions and a better performance than traditional methods in integral codes, i.e., empirical correlations, which are also much case-specific. Influence of CO present in the mixture on the PAR performance is also addressed in this paper

Producing live TV programmes on multi-mobile: an academic experience

An Academic Research Group has studied the last developments in Mobile Television and Live TV Production with conventional technologies in order to explore the possibilities of producing audiovisual formats which use several mobile telephone units as if they were on-line studio cameras. The aim is to apply these new possibilities and conditions to live team work. This plan, which has turned out to be successful, has contributed to an emerging technology-based company, which is able to provide their clients with the production of institutional and business events. These mobile telephones cameras and microphones obtain images and sounds that are transmitted via the 3G network, and are subsequently combined live and sent to a streaming server, where they are re-addressed to the client’s suggested URL, thus creating live multi-mobile programmes

Interaction of 8 He with 208Pb at near-barrier energies: 4 He and 6 He production

Spanish Ministry of Economy and Competitiveness-FPA-2010-22131-CO2-01 (FINURA) y FPA2013-47327-C2-1-RMinistry of Science and Higher Education of Poland-N202 033637National Science Centre of Poland-2013/08/M/ST2/00257 (LEA-COPIGAL) y 2014/14/M/ST2/00738 (COPIN-INFN Collaboration)European Science Foundation-EUI2009-04163432 (EUROGENESIS

Portal corporatiu : intranet per a la relació treballadors - empresa

Aplicació web per a empreses mitjanes amb un nombre considerable de treballadors per tal que puguin mantenir un control sobre les seves activitats i poder automatitzar unes altres. També serà per als treballadors ja que es fomentarà la comunicació entre iguals. Tindrem diversos tipus d'usuaris segons el seu càrrec i responsabilitat dins l'empresa, amb interfícies d'usuari diferents.Aplicación web para empresas medianas con un considerable número de trabajadores, para mantener un control y poder automatizar algunas de sus actividades. Servirá también para fomentar la comunicación entre los trabajadores. Habrá diversos tipos de usuario, según su cargo y responsabilidad en la empresa. a los que corresponderán interficies de usuario diferentes

Letter in response to article in journal of infection: “High SARS-CoV-2 antibody prevalence among healthcare workers exposed to COVID-19 patients”

Letter to editorUnidad Docente de Inmunología, Oftalmología y ORLFac. de Óptica y OptometríaTRUEpu

Metabolómica de la lágrima

Unidad Docente de Inmunología, Oftalmología y ORLFac. de Óptica y OptometríaTRUEpu