Biodegradation of dodecylbenzene solfonate sodium by Stenotrophomonas maltophilia Biofilm

Immobilization for microbial cultures has proved to be advantageous in municipal and industrial sewage treatment because of high degradation efficiency and good operational stability. In this survey, a bacterial strain was isolated from activated sludge that utilized branched anionic surfactants (BAS) as a sole carbon source. Identification of isolated strain was from 16S rRNA sequencing method. The immobilized cells on silanized glass beads as support and unmodified surfaces were used for removal of BAS; both types showed effective biodegrading of BAS. The removal rate in silanized surface was about 2 fold of unmodified surface. The result of biodegradation was studied by HPLC method and scanning electron microscope

Restoration of heart function using transplantation of human umbilical cord matrix-derived cardiomyocytes and vascular endothelial growth factor

Objectives: In the previous study, although it has been shown that intramyocardial injection of human umbilical cord matrix stem cell (hUCM) improved cardiac function 4 weeks post MI, but angiogenesis has not been observed. Angiogenesis and replacing lost cardiomyocytes with new, live cardiomyocytes are considered as two key agents in cardiac repair. To achieve the above two factors we examined the effects of combination of stem cell and angiogenic therapy approaches by simultaneously injection of hUCM-derived cardiomyocytes with vascular endothelial growth factor (VEGF) in cardiac repair. Methods: MI-induced animals(by ligation of LAD) received 50 μl PBS, 5 � 106 differentiated hUCM cells (dhUCM), 5μg VEGF in normal saline and 5 � 106 dhUCM cells combined with 5μg VEGF in normal saline, intramyocardialy. MI group, with no other intervention, served as a control group. We were assessed survival, migration and integration of dhUCM cells, as well as angiogenesis eight weeks post MI induction. Results: Eight weeks post MI, although dhUCM and VEGF groups have shown that LVEF and LVFS improved significantly, but animals in dhUCM+VEGF group have the highest rise in LVEF and LVFS in comparison to the other MI-induced groups (p<0.05). Histological and morphological analysis have revealed that myocardium of animals in dhUCM+VEGF group have the highest vascular density and the lowest fibrosis tissue in comparison to the other MI-induced groups (p<0.05). Immunohistological assessments revealed that transplanted dhUCM cells have survived, migrated to infarcted area and integrated with recipient cardiac tissue. Conclusion: we have found that intramyocardial administration of dhUCM cells combined with VEGF improved cardiac function, enhanced angiogenesis and reduced fibrosis tissue formation after MI, eight weeks post MI. © Kaveh et al

Biodegradation of dodecylbenzene solfonate sodium by Stenotrophomonas maltophilia biofilm

Immobilization for microbial cultures has proved to be advantageous in municipal and industrial sewage treatment because of high degradation efficiency and good operational stability. In this survey, a bacterial strain was isolated from activated sludge that utilized branched anionic surfactants (BAS) as a sole carbon source. Identification of isolated strain was from 16S rRNA sequencing method. The immobilized cells on silanized glass beads as support and unmodified surfaces were used for removal of BAS; both types showed effective biodegrading of BAS. The removal rate in silanized surface was about 2 fold of unmodified surface. The result of biodegradation was studied by HPLC method and scanning electron microscope. © 2010 Academic Journals