Functionalized Conjugated PolyelectrolytesDesign and Biomedical Applications /

VIII, 87 p. 62 illus., 20 illus. in color.online

Spatio-Temporal Evolution and Development Path of Industry–University–Research Cooperation and Economic Vulnerability: Evidence from China’s Yangtze River Economic Belt

This study explores the impact of industry–university–research (IUR) cooperation on the economic system. The study constructs a vulnerability evaluation index comprising 42 indicators in five dimensions. The Yangtze River Economic Belt (YREB) panel data from 2006–2018 are used to assess economic vulnerability in China. The research results show that, firstly, from 2006–2018, the index values indicated a good development trend, with fluctuations in the values of the sensitivity and economic vulnerability indices. The response ability index values gradually increased, especially later in this period. This showed a promising trend of low sensitivity, high response ability, and low vulnerability. Secondly, the spatial distribution pattern demonstrated certain laws and continuity, but the development process was unstable, while the eastern < the central < the western showed a “ladder” evolution pattern, “extremely poor” characteristics, and an “agglomeration” evolutionary path. Thirdly, the impact of IUR cooperation on regional economic development can be divided into three phases: knowledge interaction, creation, and application. The interaction and synergy between universities, research institutions, and enterprises play a role in regional economic development. The study recommends strengthening the coupling coordination of the production, research and development (R&D), cooperation, and innovation, with the dual functions of government guidance and market decision making

Spatio-Temporal Evolution and Development Path of Industry–University–Research Cooperation and Economic Vulnerability: Evidence from China’s Yangtze River Economic Belt

This study explores the impact of industry–university–research (IUR) cooperation on the economic system. The study constructs a vulnerability evaluation index comprising 42 indicators in five dimensions. The Yangtze River Economic Belt (YREB) panel data from 2006–2018 are used to assess economic vulnerability in China. The research results show that, firstly, from 2006–2018, the index values indicated a good development trend, with fluctuations in the values of the sensitivity and economic vulnerability indices. The response ability index values gradually increased, especially later in this period. This showed a promising trend of low sensitivity, high response ability, and low vulnerability. Secondly, the spatial distribution pattern demonstrated certain laws and continuity, but the development process was unstable, while the eastern < the central < the western showed a “ladder” evolution pattern, “extremely poor” characteristics, and an “agglomeration” evolutionary path. Thirdly, the impact of IUR cooperation on regional economic development can be divided into three phases: knowledge interaction, creation, and application. The interaction and synergy between universities, research institutions, and enterprises play a role in regional economic development. The study recommends strengthening the coupling coordination of the production, research and development (R&D), cooperation, and innovation, with the dual functions of government guidance and market decision making

K-Functionalized Carbon Quantum Dots-Induced Interface Assembly of Carbon Nanocages for Ultrastable Potassium Storage Performance

Carbon nanocages (CNCs), with unique merits of morphology and structure, have attracted increasing attention for energy storage and conversion. However, the synthesis of CNCs reported so far suffers from relatively harsh conditions and expensive raw materials. Herein, porous CNCs are intelligently designed using low-cost glucose as the carbon precursor via a facile K-functionalized carbon quantum dots (K-CQDs)-induced assembly route under hydrothermal process. The resulting CNCs have a unique cage-like structure, large surface area, and rich carboxyl groups. With these elegant structural merits, the as-made CNCs anode shows a high reversible capacity of 270 mAh g−1 at 100 mA g−1 after 200 cycles and a long-term cycling stability of 206 mAh g−1 at 2000 mA g−1 after 4000 cycles. An intercalation reaction mechanism with the K+ intercalation compound is further identified through an in-situ Raman technique. Density functional theory simulations reveal that abundant carboxyl groups inherited from K-CQDs can significantly promote the potassium storage capacities of the CNCs electrode.</p

Polarity Conversion of Conjugated Polymer for Lysosome Escaping

Polymers are mostly trapped in lysosomes when they enter cells and are then expelled, otherwise they were designed to be degradable to small molecules or to sabotage lysosomes. Therefore, they have reached the limit of the unique functionalities as a whole. Different from other escaping strategies, we introduced the polarity exchanging approach to rigid-backboned conjugated polymer for controlled penetrating through endosome or lysosome membranes. With the aid of pH-sensitive cleavage of water-soluble side chain, the rigid conjugated polymer turns highly hydrophobic after it is internalized into lysosomes and then accomplishes escaping. Thus, polarity exchange of CPs could become a new strategy for their application on chemotherapeutics

Artificial regulation of state transition for augmenting plant photosynthesis using synthetic light-harvesting polymer materials

Artificial regulation of state transition between photosystem I (PSI) and PSII will be a smart and promising way to improve efficiency of natural photosynthesis. In this work, we found that a synthetic light-harvesting polymer [poly(boron-dipyrromethene-co-fluorene) (PBF)] with green light absorption and far-red emission could improve PSI activity of algae Chlorella pyrenoidosa, followed by further upgrading PSII activity to augment natural photosynthesis. For light-dependent reactions, PBF accelerated photosynthetic electron transfer, and the productions of oxygen, ATP and NADPH were increased by 120, 97, and 76%, respectively. For light-independent reactions, the RuBisCO activity was enhanced by 1.5-fold, while the expression levels of rbcL encoding RuBisCO and prk encoding phosphoribulokinase were up-regulated by 2.6 and 1.5-fold, respectively. Furthermore, PBF could be absorbed by the Arabidopsis thaliana to speed up cell mitosis and enhance photosynthesis. By improving the efficiency of natural photosynthesis, synthetic light-harvesting polymer materials show promising potential applications for biofuel production

Cross-Linking of Thiolated Paclitaxel-Oligo(p-phenylene vinylene) Conjugates Aggregates inside Tumor Cells Leads to "Chemical Locks" That Increase Drug Efficacy

How to reduce the resistance of certain tumor cells to paclitaxel (PTX) and related taxoid anticancer drugs is a major challenge for improving cure rates. An oligo(p-phenylenevinylene) unit with thiol groups and a PTX unit (OPV-S-PTX), which enhances drug efficacy and reverses resistance is thus designed. The mechanism involves diffusion of OPV-S-PTX into the cell, where pi-pi interactions lead to aggregation. Cross-linking of the aggregates via oxidation of thiol groups is favored in tumor cells because of the higher reactive oxygen species (ROS) concentration. Cross-linked aggregates &quot;chemically lock&quot; the multichromophore particle for a more persistent effect. The IC50 of OPV-S-PTX for tumor cell line A549 is reduced down to 0.33 x 10(-9) M from that observed for PTX itself (41 x 10(-9) M). Enhanced efficacy by OPV-S-PTX is proposed to proceed via acceleration of microtubule bundle formation. A549/T-inoculated xenograft mice experiments reveal suppression of tumor growth upon OPV-S-PTX treatment. Altogether, these results show that the internal cross-linking of OPV-S-PTX through ROS provides a means to discriminate between tumor and healthy cells and the formation of the chemically locked particles enhances drug efficacy and helps in reducing resistance.</p

Solar-Driven Producing of Value-Added Chemicals with Organic Semiconductor-Bacteria Biohybrid System

Photosynthetic biohybrid systems exhibit promising performance in biosynthesis; however, these systems can only produce a single metabolite and cannot further transform carbon sources into highly valuable chemical production. Herein, a photosynthetic biohybrid system integrating biological and chemical cascade synthesis was developed for solar-driven conversion of glucose to value-added chemicals. A new ternary cooperative biohybrid system, namely bacterial factory, was constructed by self-assembling of enzyme-modified light-harvesting donor-acceptor conjugated polymer nanoparticles (D-A CPNs) and genetically engineered Escherichia coli (E. coli). The D-A CPNs coating on E. coli could effectively generate electrons under light irradiation, which were transferred into E. coli to promote the 37% increment of threonine production by increasing the ratio of nicotinamide adenine dinucleotide phosphate (NADPH). Subsequently, the metabolized threonine was catalyzed by threonine deaminase covalently linking with D-A CPNs to obtain 2-oxobutyrate, which is an important precursor of drugs and chemicals. The 2-oxobutyrate yield under light irradiation is increased by 58% in comparison to that in dark. This work provides a new organic semiconductor-microorganism photosynthetic biohybrid system for biological and chemical cascade synthesis of highly valuable chemicals by taking advantage of renewable carbon sources and solar energy

Photoactive conjugated polymer-based strategy to effectively inactivate RNA viruses

In this work, a photodynamic therapy system based on conjugated polymers (CPs) is developed to inhibit the infection of RNA viruses. Three cationic CPs with different backbone structures fluorene-co-phenylene (PFP), thiophene (PMNT), and phenylene vinylene (PPV) exhibit different photoinactivation effects. PPV and PMNT cause effective inactivation of viruses under light irradiation, while SARS-CoV-2 pseudotyped viruses keep infectious after treated by PFP, which is determined by the interactions between CPs with the proteins and gene of viruses. This work preliminarily reveals the effect of CP-virus interactions on their photoinactivation activity and would be beneficial to develop high-efficient antiviral PDT agents